The XIST locus replicates late on the active X, and earlier on the inactive X based on FISH DNA replication analysis of somatic cell hybrids

1995 ◽  
Vol 21 (5) ◽  
pp. 327-333 ◽  
Author(s):  
Beth S. Torchia ◽  
Barbara R. Migeon
Keyword(s):  
Dna Replication ◽  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Replication Analysis ◽  
Xist Locus

scholarly journals The Human β-Globin Replication Initiation Region Consists of Two Modular Independent Replicators

2004 ◽  
Vol 24 (8) ◽  
pp. 3373-3386 ◽  
Author(s):  
Lixin Wang ◽  
Chii-Mei Lin ◽  
Sarah Brooks ◽  
Dan Cimbora ◽  
Mark Groudine ◽  
...  
Keyword(s):  
Dna Replication ◽  
Somatic Cell ◽  
Detailed Analysis ◽  
Mammalian Cells ◽  
Replication Initiation ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Initiation Of Dna Replication ◽  
Specific Sequences

ABSTRACT Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation of DNA replication from the human β-globin locus. This analysis suggests that the β-globin replication initiation region contains two adjacent, redundant replicators. Each replicator was capable of initiating DNA replication independently at ectopic sites. Within each of these two replicators, we identified short, discrete, nonredundant sequences, which cooperatively determine replicator activity. Experiments with somatic cell hybrids further demonstrated that the requirements for initiation at ectopic sites were similar to the requirements for initiation within native human chromosomes. The replicator clustering and redundancy exemplified in the human β-globin locus may account for the extreme difficulty in identifying replicator sequences in mammalian cells and suggest that mammalian replication initiation sites may be determined by cooperative sequence modules.

Novel strategies for eutherian x marsupial somatic cell hybrids: mapping the genome of Monodelphisdomestica

1997 ◽  
Vol 76 (3-4) ◽  
pp. 115-122 ◽  
Author(s):  
T.B. Nesterova ◽  
A.A. Isaenko ◽  
N.M. Matveeva ◽  
A.G. Shilov ◽  
N.B. Rubtsov ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Extinction of expression of the translocatedmyc gene in somatic cell hybrids between mouse myeloma and l-cells

1989 ◽  
Vol 43 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Aviva Greenberg ◽  
Mohammad Huazzi ◽  
Hava Sharir ◽  
Lea Cohen ◽  
Yehudit Bergman ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
L Cells ◽  
Mouse Myeloma
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