Simulated counter-current moving column chromatography used in the continuous separation of carbohydrate mixtures

A new megastigmane derivative, (6 R,9 S)–6′-(4″-hydroxybenzoyl)-roseoside (1) and two known compounds, the biflavoneagathisflavone (2) and 4-hydroxy-benzoic acid (3) were isolated and purified from leaves and stems of Ouratea polyantha Engl. Agathisflavone was isolated in a single high-speed counter-current chromatography run, while the megastigmane was purified in two steps, by using a combination of high-speed countercurrent chromatography and analytical column chromatography. All structures were elucidated on the basis of spectral evidence and comparison with literature data. Compound 1 was characterized by [α]D20, UV-Vis, IR, MS, 1H NMR, 13C NMR, HMQC, HMBC, COSY and NOESY. Compounds 1 and 2 showed an inhibitory effect of 63.6 and 13.7% on the G-6-Pase intact microsomes, respectively.

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Heparin Excretion in Intact and Hepatectomized Rats

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650152 ◽

1981 ◽

Vol 45 (02) ◽

pp. 146-149

Author(s):

Ray Losito ◽

Harry Gattiker ◽

Ginette Bilodeau

Keyword(s):

Column Chromatography ◽

Normal Values ◽

Anticoagulant Activity ◽

Biliary Fistula ◽

Kinetics Of ◽

Intact Rats

SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

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Extraction of lanthanons with alkali metal amalgams. II. A counter-current process for the extraction of samarium or ytterbium

Journal of Biochemical Toxicology ◽

10.1002/jbt.2570130102 ◽

1963 ◽

Vol 13 (1) ◽

pp. 7-12

Author(s):

M. F. Barrett ◽

N. E. Topp

Keyword(s):

Alkali Metal ◽

Current Process ◽

Counter Current

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VANILLINSÄURE ALS ENDPRODUKT DES STOFFWECHSELS VON ADRENALIN UND NORADRENALIN. II.

Acta Endocrinologica ◽

10.1530/acta.0.0440101 ◽

1963 ◽

Vol 44 (1) ◽

pp. 101-106 ◽

Cited By ~ 4

Author(s):

Wilhelm Dirscherl ◽

Helmut Thomas

Keyword(s):

Rat Liver ◽

Paper Chromatography ◽

Column Chromatography ◽

Vanillic Acid ◽

Hippuric Acid ◽

Single Spot ◽

Solvent Systems ◽

Perfusion Experiment ◽

Kinetics Of

ABSTRACT Perfusion of rat liver with vanillic acid yielded only one metabolite. In paper chromatography with three different solvent systems, the substance showed the same RF-values as vanillyolglycine (3-methoxy-4-hydroxyhippuric acid) and in mixed chromatograms there was only one single spot. After separation by column chromatography, the UV- and IRspectra of the reaction product were identical with those of 3-methoxy4-hydroxy-hippuric acid. During the perfusion experiment, the kinetics of the conjugation were investigated.

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