VANILLINSÄURE ALS ENDPRODUKT DES STOFFWECHSELS VON ADRENALIN UND NORADRENALIN. II.

ABSTRACT Perfusion of rat liver with vanillic acid yielded only one metabolite. In paper chromatography with three different solvent systems, the substance showed the same RF-values as vanillyolglycine (3-methoxy-4-hydroxyhippuric acid) and in mixed chromatograms there was only one single spot. After separation by column chromatography, the UV- and IRspectra of the reaction product were identical with those of 3-methoxy4-hydroxy-hippuric acid. During the perfusion experiment, the kinetics of the conjugation were investigated.

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VANILLINSÄURE ALS ENDPRODUKT DES ABBAUES VON ADRENALIN UND NORADRENALIN

Acta Endocrinologica ◽

10.1530/acta.0.0390385 ◽

1962 ◽

Vol 39 (3) ◽

pp. 385-394 ◽

Cited By ~ 16

Author(s):

Wilhelm Dirscherl ◽

Helmut Thomas ◽

Herbert Schriefers

Keyword(s):

Melting Point ◽

Ir Spectra ◽

Paper Chromatography ◽

Column Chromatography ◽

Human Urine ◽

Vanillic Acid ◽

Mandelic Acid ◽

Uv And Ir Spectra ◽

Rat Livers

ABSTRACT In rat livers perfused with 3-methoxy-4-hydroxy-mandelic acid, formerly believed to be the endproduct of the metabolism of adrenaline and noradrenaline, three metabolites were detected by paper chromatography. After further purification by column chromatography, one of these substances was identified by melting point, shape of crystals, UV- and IR-spectra as vanillic acid. Perfusion with vanillic acid yielded only one metabolite in very small quantities. Because of its properties it is assumed to be a conjugate of vanillic acid. Hence vanillic acid, isolated in 1959 from human urine by Dirscherl & Schmidtmann, is the actual endproduct of the metabolism of adrenaline and noradrenaline.

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VANILLINSÄURE ALS ENDPRODUKT DES ABBAUES VON ADRENALIN UND NORADRENALIN

Acta Endocrinologica ◽

10.1530/acta.0.0450641 ◽

1964 ◽

Vol 45 (4) ◽

pp. 641-646 ◽

Cited By ~ 8

Author(s):

Wilhelm Dirscherl ◽

Betty Brisse

Keyword(s):

Melting Point ◽

Rat Liver ◽

Vanillic Acid ◽

Human Liver ◽

Mandelic Acid ◽

Post Mortem ◽

The Third ◽

Uv And Ir Spectra ◽

Solvent Systems ◽

Liver Homogenates

ABSTRACT Incubation of homogenates of rat liver or human liver with D,L-3-methoxy-4-hydroxy-mandelic acid yielded 3 diazopositive compounds. In addition to the starting material, vanillic acid could be isolated by means of column chromatography. It was identified by the shape of its crystals, micro melting point, mixed melting point, UV- and IR-spectra, and paper chromatography in 3 different solvent systems. The third substance has not yet been identified. Since acid hydrolysis does not result in any cleavage a conjugate can be ruled out. The yield of vanillic acid was about 12 per cent with rat liver, 3 per cent with post mortem liver and 7-8 per cent with liver obtained at operation. Vanillic acid is not further metabolized by liver homogenates and can also be considered as the final metabolite of adrenaline and noradrenaline in the human liver.

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The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers

Biochemical Journal ◽

10.1042/bj1860235 ◽

1980 ◽

Vol 186 (1) ◽

pp. 235-242 ◽

Cited By ~ 6

Author(s):

M J Connor ◽

J A Blair

Keyword(s):

Ion Exchange ◽

Rat Liver ◽

Paper Chromatography ◽

Column Chromatography ◽

Lactobacillus Casei ◽

Deae Cellulose ◽

Microbiological Assay ◽

Exchange Column ◽

Chromatographic Techniques ◽

Ion Exchange Column

About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labelled folate was incorporated into two folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, though less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0nmol/g) and 10-formylpteroyltetraglutamate (0.25nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase treatment.

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Heparin Excretion in Intact and Hepatectomized Rats

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650152 ◽

1981 ◽

Vol 45 (02) ◽

pp. 146-149

Author(s):

Ray Losito ◽

Harry Gattiker ◽

Ginette Bilodeau

Keyword(s):

Column Chromatography ◽

Normal Values ◽

Anticoagulant Activity ◽

Biliary Fistula ◽

Kinetics Of ◽

Intact Rats

SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

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