The effect of growth regulators on meristem tip development and in vitro multiplication ofSolanum tuberosum L. plants

1980 ◽  
Vol 23 (2) ◽  
pp. 155-166 ◽  
Author(s):  
F. J. Novák ◽  
J. Zadina ◽  
V. Horáĉková ◽  
I. Maŝková
Keyword(s):  
Growth Regulators ◽  
In Vitro Multiplication

Effect of growth regulators on in vitro multiplication and rooting of shoot cultures in sugarcane

Sugar Tech ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 86-88 ◽  
Author(s):  
Shweta Pathak ◽  
M. Lal ◽  
A. K. Tiwan ◽  
M. L. Sharma
Keyword(s):  
Growth Regulators ◽  
Shoot Cultures ◽  
In Vitro Multiplication

In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators

2019 ◽  
Vol 15 (1) ◽  
pp. 1-7
Author(s):  
Daniele Camargo Nascimento ◽  
Maximiliano Dini ◽  
Norton Victor Sampaio ◽  
Márcia Wulff Schuch
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
In Vitro Multiplication

scholarly journals In Vitro Growth and Regeneration of Drosera spatulata Labill. on Various Media

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1033-1034 ◽  
Author(s):  
Mirna Curkovic Perica ◽  
Jasna Berljak
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Sodium Hypochlorite ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Ms Medium ◽  
In Vitro Growth ◽  
In Vitro Multiplication

Conditions for in vitro multiplication and flowering of Drosera spatulata plants were established. Shoot tips of greenhouse-grown plants were sterilized with 1% or 0.5% sodium hypochlorite. The influence of different media concentrations, hormone supplementation, and pH was investigated. Full MS medium without growth regulators was the best for regeneration and multiplication of plants. Regenerated shoots rooted spontaneously on medium without growth regulators and without transfer to additional medium. In 3 months, 100 to 200 plants were generated per explant. Flowering was induced on media supplemented with plant growth regulators. Plants were acclimatized on sterile peat.

Influence of Different Growth Regulators on In Vitro Multiplication of Mixed Diploid Banana (Musa AB)

2014 ◽  
Vol 86 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Pooja Bohra ◽  
Ajit Arun Waman ◽  
B. N. Sathyanarayana ◽  
K. Umesha ◽  
Balakrishna Gowda
Keyword(s):  
Growth Regulators ◽  
In Vitro Multiplication ◽  
Diploid Banana

scholarly journals Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

2016 ◽  
Vol 37 (5) ◽  
pp. 2901
Author(s):  
Daniele Brandstetter Rodrigues ◽  
Michele Carla Nadal ◽  
Samila Silva Camargo ◽  
Adriane Marinho de Assis ◽  
Márcia Wullf Schuch ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Aerial Part ◽  
Culture Medium ◽  
Coconut Fiber ◽  
Indole Butyric Acid ◽  
In Vitro Multiplication ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Points Of View

An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L-1) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L-1) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

In vitro Multiplication and Microtuberization of Solanum tuberosum using different Growth Regulators

2018 ◽  
Vol 31 (2) ◽  
pp. 114
Author(s):  
Priyadarshani P Mohapatra ◽  
V K Batra ◽  
Subhash Kajla ◽  
Anil K Poonia
Keyword(s):  
Solanum Tuberosum ◽  
Growth Regulators ◽  
In Vitro Multiplication

scholarly journals Multiplicação in vitro de Caesalpinia pyramidalis (Leguminosae)

2013 ◽  
Vol 13 ◽  
Author(s):  
Tecla Dos Santos Silva ◽  
Cristina Ferreira Nepomuceno ◽  
Bárbara Paula dos Santos Borges ◽  
Bruno Freitas Matos Alvim ◽  
José Raniere Ferreira De Santana
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Medium ◽  
Dry Mass ◽  
Shoot Length ◽  
Nodal Segments ◽  
In Vitro Multiplication ◽  
Different Types

Caesalpinia pyramidalis is a species endemic to the Caatinga and known popularly as catingueira, which is widely used by local people, mainly for its timber and medicinal and fodder properties. This study investigated the effects of different types and concentrations of plant growth regulators on the in vitro multiplication of C. pyramidalis. In the first experiment, nodal segments were inoculated in media containing different combinations (0.0–8.0 µM) of BAP and NAA. In the second experiment, nodal segments wereinoculated in media containing different types (KIN, BAP and TDZ) and concentrations (0.0–16μM) of cytokinins. We used a WPM medium supplemented with 87.64 mM sucrose and solidified with 7.0 g L-1 agar. After 45 days, the highest number of shoots, leaf number, shoot length and dry mass of shoots were obtained when nodal segments were inoculated into a culture medium without plant growth regulators.

scholarly journals In Vitro Multiplication of Brazilian Ginseng (Pfaffia spp.) for in Vitro Conservation Purposes

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 753D-753
Author(s):  
Gerson Renan de Luces Fortes* ◽  
Ana Carolina Pereira e Silva
Keyword(s):  
Genetic Resources ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Shoot Growth ◽  
In Vitro Conservation ◽  
In Vitro Multiplication ◽  
Specific Protocol ◽  
Brazilian Ginseng ◽  
Anti Inflammatory

Brazilian ginseng (Pfaffia sp.) is largely known as having anabolic, analgesic, anticancerous, anti-inflammatory, antileukemic properties, and more. Embrapa Genetic Resources and Biotechnology is the major Conservation Center in Brazil. This species could only be stored under in vitro conditions. There are more than two hundred native acessions collected from different locations in the country. So far very few works are found in the literature and there is no specific protocol for in vitro conservation. The aim of this study was to test five different acessions from in vitro collection under one specific nutrient medium. The acessions were catalogued as 2205-12; 2202-04; 2209-11; 2209-10 and 2205-21. One-bud microcutting was inoculated on a MS nutrient medium without growth regulators and added with vitamins, myoinositol (100 mg·L-1), sucrose (30 mg·L-1) and agar (6 mg·L-1). The pH was adjusted to 5.7 before the agar addition. Each treatment was replicated 7 (seven) times and each plot consisted of six test tubes with 5-mL solution. The trial was replicated twice. The evaluations were performed once weekly where the shoot growth was taken along with the number of buds and roots formed. The highest shoot growth were obtained for the acessions 2205-12 and 2209-10. Acession 2209-11 had an intermediate shoot growth and number of buds, but had the highest number of roots per shoot (4.27) compared to 2.14 and 3.75 for acessions 2205-21 and 2209-10, respectively. The highest rate of multiplication was achieved for acessions 2205-12 (4.49) and 2202-04 (4.46) as compared to 3.60 for acession 2205-21. It was clearly shown that acession 2205-21 is a recalcitrant one as in vitro multiplication is concerned. All the acessions rooted in this medium.

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