Effect of growth regulators on in vitro multiplication and rooting of shoot cultures in sugarcane

Sugar Tech ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 86-88 ◽  
Author(s):  
Shweta Pathak ◽  
M. Lal ◽  
A. K. Tiwan ◽  
M. L. Sharma
Keyword(s):  
Growth Regulators ◽  
Shoot Cultures ◽  
In Vitro Multiplication

In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators

2019 ◽  
Vol 15 (1) ◽  
pp. 1-7
Author(s):  
Daniele Camargo Nascimento ◽  
Maximiliano Dini ◽  
Norton Victor Sampaio ◽  
Márcia Wulff Schuch
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
In Vitro Multiplication

scholarly journals In Vitro Growth and Regeneration of Drosera spatulata Labill. on Various Media

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1033-1034 ◽  
Author(s):  
Mirna Curkovic Perica ◽  
Jasna Berljak
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Sodium Hypochlorite ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Ms Medium ◽  
In Vitro Growth ◽  
In Vitro Multiplication

Conditions for in vitro multiplication and flowering of Drosera spatulata plants were established. Shoot tips of greenhouse-grown plants were sterilized with 1% or 0.5% sodium hypochlorite. The influence of different media concentrations, hormone supplementation, and pH was investigated. Full MS medium without growth regulators was the best for regeneration and multiplication of plants. Regenerated shoots rooted spontaneously on medium without growth regulators and without transfer to additional medium. In 3 months, 100 to 200 plants were generated per explant. Flowering was induced on media supplemented with plant growth regulators. Plants were acclimatized on sterile peat.

Influence of Different Growth Regulators on In Vitro Multiplication of Mixed Diploid Banana (Musa AB)

2014 ◽  
Vol 86 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Pooja Bohra ◽  
Ajit Arun Waman ◽  
B. N. Sathyanarayana ◽  
K. Umesha ◽  
Balakrishna Gowda
Keyword(s):  
Growth Regulators ◽  
In Vitro Multiplication ◽  
Diploid Banana

scholarly journals The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens

2008 ◽  
Vol 20 (2) ◽  
pp. 93-102 ◽  
Author(s):  
Teresa Cybularz-Urban ◽  
Ewa Hanus-Fajerska
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Plant Material ◽  
Viral Infections ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Viral Pathogens ◽  
Morphogenesis In Vitro ◽  
Different Origin

Abstract The micropropagation efficiency of four interspecific Cattleya hybrids (clones: 69, 75, 149 and 150) infected with Cymbidium mosaic (CyMV) and Odontoglossum ringspot (ORSV) viruses was assessed. The aim of experiments was to evaluate with that model to what extent viral infection affects the morphogenesis in vitro in orchid hybrids of different origin. The effectiveness of plant material exposure to therapeutic levels of plant growth regulators supplied with media in order to suppress infection was also verified. The vitality of proliferating infected shoot cultures was limited, and the symptoms of senility were frequently observed. Regardless genotype of the studied clone, during acclimation to ex vitro conditions considerable losses become visible what indicates the necessity of testing the donor material for possible latent viral infections. Infection with CyMV and ORSV mostly persisted in every tested clone.

scholarly journals Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

2016 ◽  
Vol 37 (5) ◽  
pp. 2901
Author(s):  
Daniele Brandstetter Rodrigues ◽  
Michele Carla Nadal ◽  
Samila Silva Camargo ◽  
Adriane Marinho de Assis ◽  
Márcia Wullf Schuch ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Aerial Part ◽  
Culture Medium ◽  
Coconut Fiber ◽  
Indole Butyric Acid ◽  
In Vitro Multiplication ◽  
Survival Percentage ◽  
Number Of Leaves ◽  
Points Of View

An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated- various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L-1) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L-1) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L-1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture.

The effect of growth regulators on meristem tip development and in vitro multiplication ofSolanum tuberosum L. plants

1980 ◽  
Vol 23 (2) ◽  
pp. 155-166 ◽  
Author(s):  
F. J. Novák ◽  
J. Zadina ◽  
V. Horáĉková ◽  
I. Maŝková
Keyword(s):  
Growth Regulators ◽  
In Vitro Multiplication

In vitro Multiplication and Microtuberization of Solanum tuberosum using different Growth Regulators

2018 ◽  
Vol 31 (2) ◽  
pp. 114
Author(s):  
Priyadarshani P Mohapatra ◽  
V K Batra ◽  
Subhash Kajla ◽  
Anil K Poonia
Keyword(s):  
Solanum Tuberosum ◽  
Growth Regulators ◽  
In Vitro Multiplication

scholarly journals Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

OENO One ◽  
2015 ◽  
Vol 49 (1) ◽  
pp. 37 ◽  
Author(s):  
Nadra Khan ◽  
Maqsood Ahmed ◽  
Ishfaq Hafiz ◽  
Nadeem Abbasi ◽  
Shaghef Ejaz ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Grape Cultivar ◽  
Half Strength ◽  
Number Of Shoots

<p style="text-align: justify;"><strong>Aim</strong>: To optimize the concentrations of growth regulators in the media for the proficient micropropagation of grapevine (<em>Vitis vinifera </em>L.) cv. King’s Ruby.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Apical meristems of the grape cultivar were used to establish <em>in vitro</em> shoot cultures. Nodal explants, each containing an axillary bud, taken from <em>in vitro</em> grown shoots were inoculated in shoot proliferation medium, i.e., half strength Murashige and Skoog (MS) medium supplemented with benzyl aminopurine (BAP), kinetin, glycine and gibberellic acid (GA<sub>3</sub>). A higher number of shoots (5.33) with greater shoot length (2.75 cm) was produced in the medium supplemented with 1.0 mg L<sup>-1</sup> BAP and 0.1 mg L<sup>-1</sup> GA<sub>3</sub>. Calluses were induced from leaf explants taken from <em>in vitro</em> grown shoots. Callus induction was greater (73.00%) on the medium containing 2.0 mg L<sup>-1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D), 0.3 mg L<sup>-1</sup> BAP and 0.2 mg L<sup>-1</sup> α-naphthaleneacetic acid (NAA). The maximum frequency of shoot regeneration (53.33%) was achieved on the medium supplemented with 1.5 mg L<sup>-1</sup> BAP and 0.5 mg L<sup>-1</sup> NAA, and the regenerated shoots successfully formed roots on growth regulator-free half strength MS medium.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Optimizing the concentration of BAP and GA<sub>3</sub> and omitting the glycine and kinetin in the culture medium increased the number and length of shoots. Similarly, for inducing the callus of the leaf explants, taken from <em>in vitro</em> grown shoots, it is recommended to adjust the medium with the higher concentration of 2,4-D and lower concentrations of BAP. Moreover, the maximum number of shoots was regenerated on a medium supplemented with relatively high levels of both BAP and NAA (1.5 and 0.5 mg L<sup>-1</sup>, respectively). Finally, we suggest the half strength MS medium that is free from growth regulators for the root formation of the regenerated shoots.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Optimizing the concentration of growth regulators is crucial for the efficient micropropagation of a grape cultivar. Knowing the specific balance between the growth regulators is necessary to establish <em>in vitro</em> shoot cultures, callus induction and shoot regeneration and, hence, to propagate disease-free true to type grape cultivars in a short time.</p>

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