scholarly journals A simple non-destructive technique for healthy and diseased leaf area assessment of potato plants

1995 ◽  
Vol 38 (4) ◽  
pp. 343-343
Author(s):  
J. T. Korva ◽  
G. A. Forbes
2020 ◽  
Vol 110 (10) ◽  
pp. 1668-1679
Author(s):  
James O. Nyanapah ◽  
Patrick O. Ayiecho ◽  
Julius O. Nyabundi ◽  
Washington Otieno ◽  
Peter S. Ojiambo

Forty-eight inbred lines of maize with varying levels of resistance to gray leaf spot (GLS) were artificially inoculated with Cercospora zeina and evaluated to characterize partial disease resistance in maize under field conditions from 2012 to 2014 across 12 environments in western Kenya. Eight measures of disease epidemic—that is, final percent diseased leaf area (FPDLA), standardized area under the disease progress curve (SAUDPC), weighted mean absolute rate of disease increase (ρ), disease severity scale (CDSG), percent diseased leaf area at the inflection point (PDLAIP), SAUDPC at the inflection point (SAUDPCIP), time from inoculation to transition of disease progress from the increasing to the decreasing phase of epidemic increase (TIP), and latent period (LP)—were examined. Inbred lines significantly (P < 0.05) affected all measures of disease epidemic except ρ. However, the proportion of the variation attributed to the analysis of variance model was most strongly associated with SAUDPC (R2 = 89.4%). Inbred lines were also most consistently ranked for disease resistance based on SAUDPC. Although SAUDPC was deemed the most useful variable for quantifying partial resistance in the test genotypes, the proportion of the variation in SAUDPC in each plot was most strongly (R2 = 93.9%) explained by disease ratings taken between the VT and R4 stages of plant development. Individual disease ratings at the R4 stage of plant development were nearly as effective as SAUDPC in discerning the differential reaction of test genotypes. Thus, GLS rankings of inbred lines based on disease ratings at these plant developmental stages should be useful in prebreeding nurseries and preliminary evaluation trials involving large germplasm populations.


2001 ◽  
Vol 91 (1) ◽  
pp. 63-69 ◽  
Author(s):  
K. W. Seebold ◽  
T. A. Kucharek ◽  
L. E. Datnoff ◽  
F. J. Correa-Victoria ◽  
M. A. Marchetti

The application of silicon (Si) fertilizers reduces the severity of blast, caused by Magnaporthe grisea, in irrigated and upland rice; however, little research has been conducted to examine the epidemiological and etiological components of this reduction. Four cultivars of rice with differential susceptibilities to race IB-49 of M. grisea were fertilized with three rates of a calcium silicate fertilizer and inoculated with the pathogen to test the effects of Si on the following components of resistance to blast: incubation period, latent period, infection efficiency, lesion size, rate of lesion expansion, sporulation per lesion, and diseased leaf area. For each cultivar, the incubation period was lengthened by increased rates of Si, and the numbers of sporulating lesions, lesion size, rate of lesion expansion, diseased leaf area, and number of spores per lesion were reduced. Lesion size and sporulation per lesion were lowered by 30 to 45%, and the number of sporulating lesions per leaf and diseased leaf area were significantly reduced at the highest rate of Si. The net effect of Si on these components of resistance is an overall reduction in the production of conidia on plants infected with M. grisea, thereby slowing the epidemic rate of blast.


2009 ◽  
Vol 99 (3) ◽  
pp. 243-250 ◽  
Author(s):  
J. P. Suh ◽  
J. H. Roh ◽  
Y. C. Cho ◽  
S. S. Han ◽  
Y. G. Kim ◽  
...  

Rice blast severely reduces production in both irrigated and water-stressed upland ecosystems of tropical and temperate countries. Nearly 50 blast resistance genes have been identified and some of those are incorporated into several rice cultivars. However, most of the resistance genes break down in a few years because of their race specificity and the rapid change in pathogenicity of the blast fungus (Magnaporthe grisea). The objective of this study was to analyze advanced backcross breeding lines (ABL) possessing the gene Pi40 for durable rice blast resistance. In all, 4 resistant genotypes, 4 japonica cultivars, and 10 monogenic differential rice genotypes with some known resistance genes were bioassayed in the greenhouse using seven sequential plantings and 29 virulent M. grisea isolates of Korea. The genotypes with the Pi40 gene had <3% diseased leaf area, which was significantly below the disease threshold level of 40% considered for durable blast resistance. Moreover, the genotypes with the Pi40 gene expressed compatibility with only two to three virulent M. grisea isolates supporting durability of resistance, in contrast to susceptible cultivars with >50% diseased leaf area and 10 compatible isolates. Of the 10 known resistance genes tested, Piz-t, Piz-5, and Pi9 showed differential reactions to the pathogen isolates in seven plantings. Genotyping of the ABL with 260 simple sequence repeat (SSR) markers revealed rapid conversion toward recurrent parent genotypes with fewer donor chromosomal segments (5.3 to 14.5%). Our study based on a sequential testing and background selection of breeding lines with the resistance gene Pi40 provided valuable information for durable blast resistance breeding in rice.


2013 ◽  
Vol 13 (3) ◽  
pp. 178-185
Author(s):  
Rafael Augusto da Costa Parrella ◽  
João Bosco dos Santos ◽  
Nádia Nardely Lacerda Durães Parrella ◽  
Diego Velásquez Faleiro e Silva

This study compared severity of angular leaf spot in common bean lines, based on the healthy and diseased leaf area, and the graded scale. We used 12 common bean lines in the dry and rainy seasons. Two contiguous experiments were conducted in each season, with and without chemical control of the pathogen. We evaluated the percentage of the healthy and diseased leaf area; severity based on a graded scale and the area under the disease progress curve; and yield. The diseased or healthy leaf area is efficient to evaluate the severity of angular leaf spot with a sample of 20 to 30 leaflets per plot. For all traits, the results of central and border areas did not differ, indicating that the evaluation of border rows is unnecessary and, finally, the severity assessment of the upper plant half can discriminate the lines more efficiently.


1987 ◽  
Vol 158 (2) ◽  
pp. 96-98 ◽  
Author(s):  
K. Manian ◽  
K. Balakrishnan ◽  
Natarajaratnam ◽  
K. Mohanasundaram

1991 ◽  
Vol 45 (3-4) ◽  
pp. 251-254 ◽  
Author(s):  
M.V. Potdar ◽  
K.R. Pawar

FLORESTA ◽  
2019 ◽  
Vol 50 (1) ◽  
pp. 1063
Author(s):  
João Everthon da Silva Ribeiro ◽  
Francisco Romário Andrade Figueiredo ◽  
Ester Dos Santos Coêlho ◽  
Walter Esfrain Pereira ◽  
Manoel Bandeira de Albuquerque

The determination of leaf area is of fundamental importance in studies involving ecological and ecophysiological aspects of forest species. The objective of this research was to adjust an equation to determine the leaf area of Ceiba glaziovii as a function of linear measurements of leaves. Six hundred healthy leaf limbs were collected in different matrices, with different shapes and sizes, in the Mata do Pau-Ferro State Park, Areia, Paraíba state, Northeast Brazil. The maximum length (L), maximum width (W), product between length and width (L.W), and leaf area of the leaf limbs were calculated. The regression models used to construct equations were: linear, linear without intercept, quadratic, cubic, power and exponential. The criteria for choosing the best equation were based on the coefficient of determination (R²), Akaike information criterion (AIC), root mean square error (RMSE), Willmott concordance index (d) and BIAS index. All the proposed equations satisfactorily estimate the leaf area of C. glaziovii, due to their high determination coefficients (R² ≥ 0.851). The linear model without intercept, using the product between length and width (L.W), presented the best criteria to estimate the leaf area of the species, using the equation 0.4549*LW.


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