Correlation between fusaric acid production and virulence of isolates ofFusarium oxysporum that causes potato dry rot in South Africa

1998 ◽  
Vol 41 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Sonja L. Venter ◽  
P. J. Steyn
Keyword(s):  
South Africa ◽  
Acid Production ◽  
Fusaric Acid ◽  
Dry Rot ◽  
Potato Dry Rot

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Chitosan is an effective inhibitor against potato dry rot caused by Fusarium oxysporum

2021 ◽  
Vol 113 ◽  
pp. 101601
Author(s):  
Jie Ren ◽  
Jie Tong ◽  
Peihua Li ◽  
Xiaoqing Huang ◽  
Pan Dong ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Effective Inhibitor ◽  
Dry Rot ◽  
Potato Dry Rot

Deciphering the effect of FUB1 disruption on fusaric acid production and pathogenicity in Fusarium circinatum

2021 ◽  
Author(s):  
M.M. Phasha ◽  
B.D. Wingfield ◽  
M.J. Wingfield ◽  
M.P.A. Coetzee ◽  
A. Hammerbacher ◽  
...  
Keyword(s):  
Acid Production ◽  
Fusaric Acid ◽  
Fusarium Circinatum

Draft Genome Sequence of Potato Dry Rot Pathogen Fusarium sambucinum Fckl. F-4

2016 ◽  
Vol 94 (3) ◽  
pp. 266-269 ◽  
Author(s):  
Virupaksh U Patil ◽  
Vanishree G. ◽  
Vinay Sagar ◽  
SK Chakrabarti
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Fusarium Sambucinum ◽  
Dry Rot ◽  
Potato Dry Rot

scholarly journals Defense Responses of Fusarium oxysporum to 2,4-Diacetylphloroglucinol, a Broad-Spectrum Antibiotic Produced by Pseudomonas fluorescens

2004 ◽  
Vol 17 (11) ◽  
pp. 1201-1211 ◽  
Author(s):  
Alexander Schouten ◽  
Grardy van den Berg ◽  
Véronique Edel-Hermann ◽  
Christian Steinberg ◽  
Nadine Gautheron ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Broad Spectrum ◽  
Acid Production ◽  
Fusaric Acid ◽  
Geographic Origin ◽  
Intergenic Spacer ◽  
Pathogenic Fungi ◽  
Defense Responses ◽  
Broad Spectrum Antibiotic

A collection of 76 plant-pathogenic and 41 saprophytic Fusarium oxysporum strains was screened for sensitivity to 2,4-diacetylphloroglucinol (2,4-DAPG), a broad-spectrum antibiotic produced by multiple strains of antagonistic Pseudomonas fluorescens. Approximately 17% of the F. oxysporum strains were relatively tolerant to high 2,4-DAPG concentrations. Tolerance to 2,4-DAPG did not correlate with the geographic origin of the strains, formae speciales, intergenic spacer (IGS) group, or fusaric acid production levels. Biochemical analysis showed that 18 of 20 tolerant F. oxysporum strains were capable of metabolizing 2,4-DAPG. For two tolerant strains, analysis by mass spectrometry indicated that deacetylation of 2,4-DAPG to the less fungitoxic derivatives monoacetylphloroglucinol and phloroglucinol is among the initial mechanisms of 2,4-DAPG degradation. Production of fusaric acid, a known inhibitor of 2,4-DAPG biosynthesis in P. fluorescens, differed considerably among both 2,4-DAPG-sensitive and -tolerant F. oxysporum strains, indicating that fusaric acid production may be as important for 2,4-DAPG-sensitive as for -tolerant F. oxysporum strains. Whether 2,4-DAPG triggers fusaric acid production was studied for six F. oxysporum strains; 2,4-DAPG had no significant effect on fusaric acid production in four strains. In two strains, however, sublethal concentrations of 2,4-DAPG either enhanced or significantly decreased fusaric acid production. The implications of 2,4-DAPG degradation, the distribution of this trait within F. oxysporum and other plant-pathogenic fungi, and the consequences for the efficacy of biological control are discussed.

Fusaric acid production byFusarium orthoceras in vitro

1957 ◽  
Vol 13 (7) ◽  
pp. 284-284 ◽  
Author(s):  
C. S. Venkata Ram
Keyword(s):  
Acid Production ◽  
Fusaric Acid

scholarly journals First Report of Fusarium proliferatum Causing Dry Rot in Michigan Commercial Potato (Solanum tuberosum) Production

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 843-843
Author(s):  
A. Merlington ◽  
L. E. Hanson ◽  
R. Bayma ◽  
K. Hildebrandt ◽  
L. Steere ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Sodium Hypochlorite ◽  
Fusarium Proliferatum ◽  
Distilled Water ◽  
Fusarium Spp ◽  
Dry Rot ◽  
Commercial Potato ◽  
Fusarium Dry Rot ◽  
Half Strength ◽  
Potato Dry Rot

Fusarium dry rot of potato (Solanum tuberosum L.) is a postharvest disease caused by several Fusarium spp. Thirteen Fusarium spp. have been implicated in dry rot of potatoes worldwide. Among them, 11 species have been reported causing potato dry rot of seed tubers in the northern United States (1). Historically, Fusarium sambucinum was the predominant species in Michigan potato production (3). Dry rot symptomatic tubers (n = 972) were collected from Michigan commercial potato storage facilities in 2011 and 2012 to determine the composition of Fusarium spp. Sections were cut from the margins of necrotic tissue with a sterile scalpel and surface disinfested in 0.6% sodium hypochlorite for 10 s, rinsed twice in sterile distilled water, and dried on sterile filter paper. The tissue sections were plated on half-strength potato dextrose agar (PDA) amended with 0.5 g/liter of streptomycin sulfate. Dishes were incubated at 23°C in the dark for 7 days. Putative Fusarium isolates were transferred onto water agar and hyphal tips from the margin of actively growing cultures were removed with a sterile scalpel and plated to carnation leaf agar (CLA) and half-strength PDA to generate pure cultures. Seven hundred and thirty Fusarium isolates were collected using these techniques. Preliminary identification of the 730 isolates was based on colony and conidial morphology on PDA and CLA, respectively. While F. oxysporum and F. sambucinum were isolated as expected from prior reports (3), three isolates of F. proliferatum were also identified. On CLA, macroconidia of F. proliferatum were sparse, slender, and mostly straight, with three to five septae (4). Microconidia were abundant, usually single celled, oval or club-shaped in short chains or false heads on monophialides and polyphialides (4), and chlamydospores were absent. On PDA, abundant white mycelium was produced and turned violet with age. Koch's postulates were confirmed through pathogenicity testing on disease-free potato tubers cvs. Atlantic and Russet Norkotah. Tubers were surface disinfested for 10 min in 0.6% sodium hypochlorite and rinsed twice in distilled water. Three tubers of each cultivar per isolate were wounded at the apical end of the tuber to a depth of 4 to 10 mm with a 4 mm diameter cork-borer. Tubers were inoculated by inserting a mycelial plug from a 7-day-old culture grown on PDA into the wound and incubating the tubers at 20°C for 21 days. All Fusarium isolates were tested. Control tubers were inoculated by inserting a water agar plug. Pathogenicity and virulence testing were replicated three times and repeated. Tubers inoculated with F. proliferatum developed typical potato dry rot symptoms but no dry rot symptoms were observed on control tubers. Fusarium proliferatum was re-isolated from symptomatic tubers, confirming Koch's postulates. To our knowledge, this is the first report of F. proliferatum causing potato dry rot in Michigan. References: (1) E. Gachango et al. Plant Dis. 96:1767. (2) D. Geiser et al. Eur. J. Plant Pathol. 110:473, 2004. (3) M. L. Lacy and R. Hammerschmidt. Fusarium dry rot. Extension Bulletin. Retrieved from http://web1.msue.msu.edu/msue/iac/onlinepubs/pubs/E/E2448POT, 23 May 2010. (4) J. F. Leslie and B. A. Summerell. The Fusarium Laboratory Manual. Wiley-Blackwell, Hoboken, NJ, 2006.

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