Influence of cultivar, season, explant type and seaweed concentrate on potato plantlet quality

1999 ◽  
Vol 42 (1) ◽  
pp. 181-188 ◽  
Author(s):  
Britta Kowalski ◽  
Anna K. Jäger ◽  
J. Van Staden
Keyword(s):  
Explant Type ◽  
Seaweed Concentrate ◽  
Plantlet Quality ◽  
Potato Plantlet

scholarly journals Effect of explant type on the rooting and acclimatization of Dianthus serotinus Waldst. & Kit.

2014 ◽  
pp. 125-136
Author(s):  
Marija Markovic ◽  
Mihailo Grbic ◽  
Dragana Skocajic ◽  
Matilda Djukic
Keyword(s):  
Ms Medium ◽  
Explant Type ◽  
Single Node ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Terminal Buds

The effect of the concentration of MS salts and explant type on D. serotinus rooting and acclimatization was investigated in order to optimize a protocol for the micropropagation of this species. The obtained results showed that explant type as well as the concentration of MS salts had a significant effect on rooting, and the highest rooting rate (85-86,7%) was achieved when culturing single-node cuttings and terminal buds on a half-strength MS medium supplemented with 0,5 mgL-1 NAA. Nevertheless, mean number of roots per explant was higher on the MS media (15,3-18,6) than on the half-strength MS media (11,8-13,4). The best acclimatization rate was obtained in a 4:1 mixture of peat and sand (83,3-86,7%). The explant type from which in vitro plantlets developed had no effect on the acclimatization rate.

The effect of seaweed concentrate on the growth and yield of potassium stressed wheat

1989 ◽  
Vol 116 (1) ◽  
pp. 29-36 ◽  
Author(s):  
R. P. Beckett ◽  
J. van Staden
Keyword(s):  
Growth And Yield ◽  
Seaweed Concentrate

scholarly journals Induction of Callogenesis, Organogenesis, and Embryogenesis in Non-Meristematic Explants of Bleeding Heart and Evaluation of Chemical Diversity of Key Metabolites from Callus

2020 ◽  
Vol 21 (16) ◽  
pp. 5826
Author(s):  
Dariusz Kulus ◽  
Alicja Tymoszuk
Keyword(s):  
Adventitious Shoots ◽  
Leaf Explants ◽  
Chemical Diversity ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Explant Type ◽  
Leaf Petiole ◽  
Primary And Secondary Metabolites ◽  
Culture Systems ◽  
Lamprocapnos Spectabilis

Lamprocapnos spectabilis (L.) Fukuhara is a perennial plant species valued in the horticultural, cosmetic, and pharmaceutical markets. To date, however, there were no studies on tissue culture systems in this species when adjusted from non-meristematic explants. The aim of this study is to induce callogenesis, organogenesis, and somatic embryogenesis in non-meristematic explants of Lamprocapnos spectabilis ‘Alba’ cultured in various media and to analyze the chemical diversity of the produced callus. Leaf, petiole, and internode explants were cultured on the modified Murashige and Skoog (MS) medium fortified with various combinations and concentrations of 6-benzyladenine (BA), indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorphenoxyacetic acid (2,4-D), and picloram (PIC). After 10 weeks of culturing, the morphogenetic response of explants was evaluated and the concentration of chlorophylls, carotenoids, anthocyanins, and polyphenols in callus was analyzed. There was no influence of explant type on the callogenesis efficiency (62.1–65.3%). The highest fresh weight of callus was produced on leaf explants in the presence of 2,4-D or PIC. In contrast, the highest share of dry weight was found in internode-derived calli and cultured on IAA-supplemented medium (up to 30.8%). Only 2.5% of all explants regenerated adventitious shoots, while rhizogenesis was reported in 4.5% of explants. Somatic embryos were produced indirectly by 0% to 100% of explants, depending on the culture medium and explant type. The highest mean number of embryos (11.4 per explant) was found on petioles cultured in the MS medium with 0.5 mg·L−1 BA and 1.0 mg·L−1 PIC. Calli cultured in media with NAA usually contained a higher content of primary and secondary metabolites. There was also a significant impact of explant type on the content of anthocyanins, polyphenols, and carotenoids in callus. Further studies should focus on the elicitation of metabolites production in callus culture systems of the bleeding heart.

The effect of a seaweed concentrate on the in vitro growth and acclimatization of potato plantlets

1999 ◽  
Vol 42 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Britta Kowalski ◽  
Anna K. Jäger ◽  
J. Van Staden
Keyword(s):  
In Vitro Growth ◽  
Seaweed Concentrate ◽  
Potato Plantlets

The Effect of Explant Type on the Establishment of Sitka Spruce [Picea sitchensis (Bong.) Carr.] in Culture

1992 ◽  
Vol 69 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. MAC AN t-SAOIR ◽  
J. O'BRIEN ◽  
C. SELBY
Keyword(s):  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Explant Type

Evidence for rooting factors in a seaweed concentrate prepared from Ecklonia maxima

1991 ◽  
Vol 137 (3) ◽  
pp. 319-322 ◽  
Author(s):  
I.J. Crouch ◽  
J. Van Staden
Keyword(s):  
Ecklonia Maxima ◽  
Seaweed Concentrate
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