Methods to increase the starch content of potato tuber callus tissue

AbstractSoft rot is a bacterial disease that causes heavy losses in potato production worldwide. The goal of this study was to identify quantitative trait loci (QTLs) for potato tuber resistance to bacteriumDickeya solaniand for tuber starch content to study the relationship between these traits. A highly resistant diploid hybrid of potato was crossed with a susceptible hybrid to generate the F1 mapping population. Tubers that were wound-inoculated with bacteria were evaluated for disease severity expressed as the mean weight of rotted tubers, and disease incidence measured as the proportion of rotten tubers. Diversity array technology (DArTseq™) was used for genetic map construction and QTLs analysis. The most prominent QTLs for disease severity and incidence were identified in overlapping regions on potato chromosome IV and explained 22.4% and 22.9% of the phenotypic variance, respectively. The second QTL for disease severity was mapped to chromosome II and explained 16.5% of the variance. QTLs for starch content were detected on chromosomes III, V, VI, VII, VIII, IX, XI, and XII in regions different than the QTLs for soft rot resistance. Two strong and reproducible QTLs for resistance toDickeya solanion potato chromosomes IV and II might be useful for further study of candidate genes and marker development in potato breeding programs. The relationship between tuber resistance to bacteria and the starch content in potato tubers was not confirmed by QTL mapping, which makes the selection of genotypes highly resistant to soft rot with a desirable starch content feasible.

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Effect of temperature on the starch content of potato callus tissue

American Potato Journal ◽

10.1007/bf02853899 ◽

1991 ◽

Vol 68 (3) ◽

pp. 191-195 ◽

Cited By ~ 5

Author(s):

Steven R. Hagen ◽

Paul Muneta ◽

Duane LeTourneau ◽

Janice Brown

Keyword(s):

Callus Tissue ◽

Starch Content ◽

Effect Of Temperature ◽

Potato Callus

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Hydrogen peroxide increases potato tuber and stem starch content, stem diameter, and stem lignin content

American Journal of Potato Research ◽

10.1007/bf02871957 ◽

2005 ◽

Vol 82 (4) ◽

pp. 279-285 ◽

Cited By ~ 12

Author(s):

H. López-Delgado ◽

H. A. Zavaleta-Mancera ◽

M. E. Mora-Herrera ◽

M. Vázquez-Rivera ◽

F. X. Flores-Gutiérrez ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Potato Tuber ◽

Lignin Content ◽

Starch Content ◽

Stem Diameter ◽

Stem Lignin

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Analysis of quantitative trait loci (QTLs) and quantitative trait alleles (QTAs) for potato tuber yield and starch content

Theoretical and Applied Genetics ◽

10.1007/s001220050963 ◽

1998 ◽

Vol 97 (5-6) ◽

pp. 834-846 ◽

Cited By ~ 57

Author(s):

R. Schäfer-Pregl ◽

E. Ritter ◽

L. Concilio ◽

J. Hesselbach ◽

L. Lovatti ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Potato Tuber ◽

Quantitative Trait ◽

Tuber Yield ◽

Starch Content ◽

Trait Loci

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Study on Correlation between the Starch Content of Potato Tuber and its Viscoelastic Characteristics

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.591-593.2545 ◽

2012 ◽

Vol 591-593 ◽

pp. 2545-2550

Author(s):

Wen Bin Guo ◽

Chun Guang Wang ◽

Jing Jing Gao

Keyword(s):

Potato Tuber ◽

Starch Content ◽

Viscoelastic Model ◽

Viscosity Coefficient ◽

Potato Tubers ◽

Obvious Effect ◽

Viscoelastic Parameters ◽

Nonlinear Stress ◽

Viscoelastic Characteristics ◽

Water Ratio

When potato tubers had received the exogenic action, water and starch in tubers generated tougor pressure which would act on cell walls in the tuber tissue, so starch content and water ratio have an obvious effect on the mechanical characteristics of potato tubers. In order to provide a mechanical method for detecting the starch content of potato tubers, correlation between viscoelastic characteristics and starch content of tubers was studied. In the research, the tubers of Desiree potato were compressed by cylindrical probe, and showed nonlinear stress-strain curves. The viscoelastic model of potato tuber was established, and its viscoelastic parameters were determined. Meanwhlie, the starch content and water ratio of potato tuber were detected after mechanical tests, and the correlation between viscoelastic parameters and starch content was analysed. According to the results of correlation analysis, viscosity coefficient which had been obtained from the viscoelastic model was significantly correlated with starch content and water ratio. Finally, the regression equation of starch content and the viscosity coefficient was determined.

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Kualitas ubi kentang pada dosis pupuk NPK dan umur panen yang berbeda

Jurnal Agro ◽

10.15575/12292 ◽

2021 ◽

Vol 8 (1) ◽

pp. 14-24

Author(s):

Kusumiyati Kusumiyati ◽

Diky Indrawibawa ◽

Syariful Mubarok ◽

Gustiono Tegar Prasetyo

Keyword(s):

Potato Tuber ◽

Starch Content ◽

Block Design ◽

French Fries ◽

Raw Material ◽

Tuber Quality ◽

Harvesting Time ◽

Randomized Block Design ◽

Npk Fertilizer ◽

The Right

Semakin tingginya permintaan bahan baku kentang untuk french fries khususnya, perlu diimbangi oleh peningkatan suplai kentang dengan kualitas yang memenuhi persyaratan skala industri. Aplikasi dosis pupuk NPK yang tepat serta umur panen yang sesuai merupakan strategi dalam meningkatkan kualitas kentang. Penelitian bertujuan untuk menentukan dosis pupuk NPK dan umur panen yang dapat menghasilkan ubi kentang dengan kualitas olah terbaik. Percobaan dilakukan dengan menggunakan Rancangan Acak Kelompok pola faktorial dengan dua faktor dan tiga ulangan. Faktor pertama, yaitu dosis pupuk NPK (50, 100, 150% dosis rekomendasi) dan umur panen (90, 105, 120 HST). Hasil penelitian memperlihatkan bahwa perlakuan dosis pupuk NPK dan umur panen tidak memperlihatkan interaksi. Kadar air dan gula reduksi tidak dipengaruhi oleh perlakuan dosis pupuk dan umur panen. Perlakuan dosis pupuk 150 kg N ha-1, 225 kg P ha-1, dan 225 kg K ha-1 menunjukkan kadar pati ubi tertinggi dengan tingkat kerenyahan french fries paling disukai. Perlakuan umur panen 120 hari setelah tanam (HST) memberikan kadar pati dan tingkat kesukaan warna dan kerenyahan french fries paling tinggi. AbstractThe potato demand is increasing, as a raw material for french fries in particular, yet followed by potato improvement to fulfil the industry requirement. The application of NPK fertilizer in appropriate dosage and harvesting time is the essential strategy to increase potato quality. This study aimed to determine the proper dosage of NPK and harvesting time to obtain the best characteristics of potato tuber for processing. The experimental design used a factorial randomized block design consisting of two factors; NPK fertilizer dosages (50, 100, 150% recommended dosage) and harvesting time (90, 105, 120 DAP). NPK fertilizer dosage had no interaction with harvesting time. Tuber water content and reducing sugars were not affected by NPK fertilizer dosage and harvesting time. The treatment of 150 kg N ha-1, 225 kg P ha-1 and 225 kg K ha-1 had the best tuber starch content, and after processing to be french fries, it showed the most favored crispness. Meanwhile, harvesting time at 120 DAP resulted in the highest tuber starch content, french fries colour and crispness score. Giving a certain dose of NPK fertilizer will affect the quality of potato tubers. The right harvesting time affects on the potato tuber quality.

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