A shoot induction procedure altered for increased shooting efficiency of potato protoplast-derived calli (cvs. crystal and norgold russet)

1988 ◽  
Vol 31 (4) ◽  
pp. 651-658 ◽  
Author(s):  
R. J. Taylor ◽  
G. A. Secor
Keyword(s):  
Norgold Russet ◽  
Shoot Induction ◽  
Induction Procedure

An fMRI investigation of the effects of a negative mood induction procedure on self-reflection

2007 ◽  
Author(s):  
Bryan T. Denny ◽  
William M. Kelley ◽  
Joseph M. Moran ◽  
Todd F. Heatherton
Keyword(s):  
Mood Induction ◽  
Negative Mood ◽  
Induction Procedure ◽  
Self Reflection ◽  
Mood Induction Procedure

An Improved Minimal Group Induction Procedure

2006 ◽  
Author(s):  
Brad Pinter ◽  
Anthony G. Greenwald
Keyword(s):  
Induction Procedure ◽  
Minimal Group

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

Development of an Agrobacterium-mediated Transformation System for `Beurre Bosc' Pear

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461d-461
Author(s):  
Richard L. Bell ◽  
Ralph Scorza ◽  
Chinnathambi Srinivasan
Keyword(s):  
Shoot Proliferation ◽  
Induction Medium ◽  
Shoot Induction ◽  
Transformation System ◽  
Gus Histochemical Assay ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Shoot Induction Medium ◽  
Basal Salts

An efficient regeneration/transformation system was developed for `Beurre Bosc' pear. Young leaves were harvested from in vitro shoots proliferated on a medium containing MS basal salts and 5 BAP, 0.5 μM IBA, and 0.6M3. Shoot regeneration was optimized using a modification of the medium of Chevreau and Leblay (1993). Explants were cultured on shoot induction medium contained 10 μM TDZ and 1 μM IBA for 4 weeks in the dark, and then transfered to a similar, but auxinless, regeneration medium until shoots developed, usually after an additional 4 to 8 weeks. Leaf tissues were transformed by co-cultivation for 3 days with Agrobacterium tumefaciens EHA101 carrying a pGA482 plasmid containing NPTII, GUS, and rolC genes, followed by cultivation on SIM containing 300 mg/L timentin. Putative transgenic plants were selected on shoot induction medium containing 80mg/L kanamycin, and multiplied on shoot proliferation medium. Four clones were confirmed as transgenic using the GUS histochemical assay and Southern blots for the NPTII and rolC genes. Plants of each clone have been rooted and successfully transfered to the greenhouse for further analysis of gene expression.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

Inhibition of shoot induction by 5-azacytidine and 5-aza-2′-deoxycytidine in Petunia involves DNA hypomethylation

1997 ◽  
Vol 16 (10) ◽  
pp. 719-724 ◽  
Author(s):  
A. P. Prakash ◽  
P. P. Kumar
Keyword(s):  
Shoot Induction ◽  
Dna Hypomethylation

Multiple Shoot Induction from Cotyledonary Node Explants of Terminalia chebula

2003 ◽  
Vol 46 (4) ◽  
pp. 585-588 ◽  
Author(s):  
B. Shyamkumar ◽  
C. Anjaneyulu ◽  
C.C. Giri
Keyword(s):  
Cotyledonary Node ◽  
Multiple Shoot ◽  
Terminalia Chebula ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Cotyledonary Node Explants

Effect of seed tuber and seed piece size on growth and incidence of hollow heart in Norgold Russet potatoes

1982 ◽  
Vol 59 (8) ◽  
pp. 367-373 ◽  
Author(s):  
D. C. Nelson ◽  
M. C. Thoreson
Keyword(s):  
Seed Piece ◽  
Seed Tuber ◽  
Hollow Heart ◽  
Norgold Russet ◽  
Piece Size
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