Cell mediated calcification in collagen gel cultures of fetal rat calvaria cells. Loss of gap junctions precedes calcification

1989 ◽  
Vol 7 (3) ◽  
pp. 6-17 ◽  
Author(s):  
Kazuto Yamazaki ◽  
Shoichi Ichimura ◽  
Terence D Allen ◽  
Tomoyuki Nakagawa
Keyword(s):  
Gap Junctions ◽  
Collagen Gel ◽  
Rat Calvaria ◽  
Fetal Rat

Ultrastructural analysis of bone nodules formed in vitro by isolated fetal rat calvaria cells

Bone ◽  
1988 ◽  
Vol 9 (3) ◽  
pp. 155-163 ◽  
Author(s):  
U. Bhargava ◽  
M. Bar-Lev ◽  
C.G. Bellows ◽  
J.E. Aubin
Keyword(s):  
Ultrastructural Analysis ◽  
Rat Calvaria ◽  
Fetal Rat

Stimulatory effects of phenytoin on osteoblastic differentiation of fetal rat calvaria cells in culture

Bone ◽  
1999 ◽  
Vol 25 (6) ◽  
pp. 653-660 ◽  
Author(s):  
D Ikedo ◽  
K Ohishi ◽  
N Yamauchi ◽  
M Kataoka ◽  
J Kido ◽  
...  
Keyword(s):  
Osteoblastic Differentiation ◽  
Cells In Culture ◽  
Rat Calvaria ◽  
Fetal Rat

The Anabolic Effects of Progesterone on Fetal Rat Calvaria in Tissue Culture

1994 ◽  
Vol 1 (4) ◽  
pp. 302-309 ◽  
Author(s):  
Deborah L. Manzi ◽  
Carol C. Pilbeam ◽  
Lawrence G. Raisz
Keyword(s):  
Tissue Culture ◽  
Rat Calvaria ◽  
Fetal Rat

Enzymatic isolation of cells from bone: cytotoxic enzymes of bacterial collagenase

1981 ◽  
Vol 240 (5) ◽  
pp. C234-C238 ◽  
Author(s):  
T. Hefley ◽  
J. Cushing ◽  
J. S. Brand
Keyword(s):  
Cell Density ◽  
Potassium Concentration ◽  
Cell Damage ◽  
The Other ◽  
Bacterial Collagenase ◽  
Rat Calvaria ◽  
Enzymatic Isolation ◽  
Fetal Rat ◽  
Clostridium Histolyticum Collagenase ◽  
Isopycnic Centrifugation

The enzymatic isolation of cells from fetal rat calvaria is most effectively achieved with crude Clostridium histolyticum collagenase. However, this bacterial collagenase damages the cells during the digestion of the tissue. We have used cell density, as measured by isopycnic centrifugation on polysucrose gradients, as an indicator of cell damage. There are at least two enzymes in crude bacterial collagenase capable of damaging the cells in this tissue. One of these is clostripain that has been well characterized. The other cytotoxic enzyme is uncharacterized, and its effects are not evident until the clostripain activity has been inhibited by alpha-tosyl-lysyl chloromethane. The apparent activity of this second enzyme can be inhibited by withholding magnesium from the digestion medium and by increasing the potassium concentration of the digestion medium.

Characterization of Osteoblast-like Cells from Fetal Rat Calvaria

1980 ◽  
Vol 59 (11) ◽  
pp. 2006-2013 ◽  
Author(s):  
B. Kadis ◽  
J.M. Goodson ◽  
S. Offenbacher ◽  
J.W. Bruns ◽  
S. Seibert
Keyword(s):  
Rat Calvaria ◽  
Fetal Rat

Side population (SP) cells isolated from fetal rat calvaria are enriched for bone, cartilage, adipose tissue and neural progenitors

Bone ◽  
2006 ◽  
Vol 38 (5) ◽  
pp. 662-670 ◽  
Author(s):  
Shulin Zhang ◽  
Soshi Uchida ◽  
Tomoyuki Inoue ◽  
Melinda Chan ◽  
Erica Mockler ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Side Population ◽  
Neural Progenitors ◽  
Rat Calvaria ◽  
Fetal Rat
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