Alkaline phosphatase inhibition by parathyroid hormone and isoproterenol in a clonal rat osteosarcoma cell line. Possible mediation by cyclic AMP

1982 ◽  
Vol 34 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Robert J. Majeska ◽  
Gideon A. Rodan
Keyword(s):  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Phosphatase Inhibition ◽  
Alkaline Phosphatase Inhibition

Effects of parathyroid hormone on cyclic AMP-formation and cytoplasmic free Ca2+ in the osteosarcoma cell line UMR 106-01

1992 ◽  
Vol 12 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Östen Ljunggren ◽  
Hans Johansson ◽  
Ulf H. Lerner ◽  
Erik Lindh ◽  
Sverker Ljunghall
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Cell Line ◽  
Single Cells ◽  
Transient Increase ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Fura 2 ◽  
Umr 106 ◽  
Camp Formation

The effects of parathyroid hormone (PTH) on cytoplasmic free CA2+ (Cai2+) and cAMP-formation were investigated in the rat osteosarcoma cell line UMR 106-01. In fura-2 loaded adherent single cells bPTH 1–34 (10 nM−1μM) induced a rapid transient increase in Cai2+ in 11% of the studied cells. In fura-2 tracings from UMR 106-01 cells in suspension, bPTH 1–34 (0.1 μM) induced a transient increase in Cai2+ in 20% of the experiments. The transient increase in Cai2+ seen in suspensions of cells was not abolished by addition of EGTA (2.5 mM) prior to challenge with PTH, suggesting that the increase in Cai2+ was derived from intracellular stores. A marked rapid increase in cAMP-formation was observed in all experiments with cells in suspension, also in the experiments where PTH did not affect Cai2+. These data show that PTH causes a release of Ca2+ from intracellular stores in a small percentage of osteosarcoma UMR 106-01 cells, and that PTH is capable of inducing an increase in cAMP-formation without affecting Cai2+ in osteoblasts.

scholarly journals Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2

Medicines ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 61
Author(s):  
Hourei Oh ◽  
Kazuya Masuno ◽  
Nobutaka Okusa ◽  
Yoshimasa Makita ◽  
Shin-ichi Fujiwara ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Collagen Production ◽  
Human Osteosarcoma ◽  
Traditional Japanese Medicine ◽  
Human Osteosarcoma Cell

Background: Orento, a traditional Japanese medicine, is known as Kampo medicine in Japan. We investigated the possible efficacy of Kampo medicine for periodontal disease. In this study, we examined the in vitro effects of orento on the proliferation of the inflammatory cytokines interleukin (IL)-6 and IL-8, the production of type 1 collagen, and the secretion of alkaline phosphatase (ALP) in the human osteosarcoma cell line Saos-2 (Saos-2 cells). Methods: The proliferation of Saos-2 cells was assessed by MTT assay. IL-6 and IL-8 levels, type 1 collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. Saos-2 cells were treated with or without 0.1, 1, 10, 100 and 1000 μg/mL of orento for 24 h. Results: Orento (10 μg/mL) significantly induced the proliferation of Saos-2 cells. At this concentration, orento suppressed IL-6 and IL-8 and enhanced type 1 collagen production and ALP secretion. Conclusions: These results indicate that orento controls the IL-6 and IL-8 secretion and cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

Possible involvement of protein' kinase C in parathyroid hormone degradation by osteoblast-like rat osteosarcoma cell line UMR106

1987 ◽  
Vol 143 (2) ◽  
pp. 539-544 ◽  
Author(s):  
Toru Yamaguchi ◽  
Hisamitsu Baba ◽  
Masaaki Fukase ◽  
Yoshikazu Kinoshita ◽  
Tadao Fujimi ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Parathyroid Hormone ◽  
Protein Kinase ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Kinase C

Degrading activity for human parathyroid hormone [PTH-(1–84)] in rat osteoblast-like osteosarcoma cell line UMR106

1986 ◽  
Vol 141 (2) ◽  
pp. 762-768 ◽  
Author(s):  
Toru Yamaguchi ◽  
Hisamitsu Baba ◽  
Masaaki Fukase ◽  
Yoshikazu Kinoshita ◽  
Tadao Fujimi ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Osteosarcoma Cell Line ◽  
Osteosarcoma Cell ◽  
Human Parathyroid Hormone ◽  
Rat Osteoblast
Sign in / Sign up

Export Citation Format

Share Document