Effect of amplification ofdhfr andlac Z genes on growth and β-galactosidase expression in suspension cultures of recombinant CHO cells

1992 ◽  
Vol 9 (1-3) ◽  
pp. 237-245 ◽  
Author(s):  
Man Bock Gu ◽  
Jeffrey A. Kern ◽  
Paul Todd ◽  
Dhinakar S. Kompala
Keyword(s):  
Cho Cells ◽  
Suspension Cultures ◽  
Recombinant Cho Cells

Expression of human Mn SOD in Chinese hamster ovary cells confers protection from oxidant injury

1993 ◽  
Vol 264 (6) ◽  
pp. L598-L605
Author(s):  
B. Warner ◽  
R. Papes ◽  
M. Heile ◽  
D. Spitz ◽  
J. Wispe
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster Ovary Cells ◽  
Lethal Dose ◽  
Chinese Hamster ◽  
Eukaryotic Expression ◽  
Wild Type ◽  
Oxidant Injury ◽  
Sod Activity ◽  
Recombinant Cho Cells

Manganese superoxide dismutase (Mn SOD) is an important component of antioxidant defense in aerobic cells because of its location in the mitochondria, a significant source of oxygen radicals and an important target of oxidant injury. To test the hypothesis that increased mitochondrial Mn SOD protects from oxidant injury, Chinese hamster ovary (CHO) cells were transfected with a eukaryotic expression vector containing the human Mn SOD cDNA. In recombinant CHO cells, Mn SOD activity was increased threefold over wild-type controls. Acute survival during paraquat exposure (0–500 microM) was significantly improved in CHO cells expressing human Mn SOD, with 71% of recombinant CHO cells surviving at the 50% lethal dose (LD50) for wild-type CHO controls. Cell growth following exposure to paraquat (100 microM) was also significantly improved in recombinant CHO cells. CHO cells expressing human Mn SOD continued to grow and divide after paraquat exposure, whereas growth of wild-type CHO cells was negligible. Protection against oxidant-induced injury was directly related to increased Mn SOD, occurring in the absence of changes in other antioxidant enzymes including catalase, Cu,Zn SOD, and glutathione associated cellular antioxidant mechanisms. We conclude that increased expression of human Mn SOD in vitro directly confers protection against oxidant injury.

scholarly journals Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potential

2007 ◽  
Vol 129 (4) ◽  
pp. 651-657 ◽  
Author(s):  
Georg Hinterkörner ◽  
Gudrun Brugger ◽  
Dethardt Müller ◽  
Friedemann Hesse ◽  
Renate Kunert ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Energy Metabolism ◽  
Mitochondrial Membrane Potential ◽  
Cell Sorting ◽  
Cho Cells ◽  
Mitochondrial Membrane ◽  
Recombinant Cho Cells

Genetic engineering of ?2,6-sialyltransferase in recombinant CHO cells and its effects on the sialylation of recombinant interferon-?

1996 ◽  
Vol 22 (1-3) ◽  
pp. 197-203 ◽  
Author(s):  
Lucia Monaco ◽  
Annie Marc ◽  
Alex Eon-Duval ◽  
Giulia Acerbis ◽  
Gianfranco Distefano ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Cho Cells ◽  
Recombinant Interferon ◽  
Recombinant Cho Cells

HIGH DENSITY AGGREGATE CULTURE OF RECOMBINANT CHO CELLS IN FLUIDIZED BED BIOREACTORS

1992 ◽  
pp. 421-423 ◽  
Author(s):  
M. Reiter ◽  
G. Blüml ◽  
T. Gaida ◽  
N. Zach ◽  
C. Schmatz ◽  
...  
Keyword(s):  
Fluidized Bed ◽  
Cho Cells ◽  
High Density ◽  
Recombinant Cho Cells ◽  
Aggregate Culture

Production of an α-Amidating Enzyme (α-AE) in Recombinant CHO Cells

1993 ◽  
pp. 493-499 ◽  
Author(s):  
K. Furukawa ◽  
K. Okuno ◽  
S. Onai ◽  
K. Sugimura ◽  
Y. Yoko-O ◽  
...  
Keyword(s):  
Cho Cells ◽  
Recombinant Cho Cells

Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells

1998 ◽  
Vol 26 (2) ◽  
pp. S115-S115 ◽  
Author(s):  
Nigel Jenkins ◽  
Lorraine Buckberry ◽  
Annie Marc ◽  
Lucia Monaco
Keyword(s):  
Genetic Engineering ◽  
Cho Cells ◽  
Recombinant Cho Cells

Effect of Doxycycline-Regulated Calnexin and Calreticulin Expression on Specific Thrombopoietin Productivity of Recombinant CHO Cells

2005 ◽  
pp. 475-478
Author(s):  
Joo Young Chung ◽  
Seung Wook Lim ◽  
Yeon Joo Hong ◽  
Yeo Wook Koh ◽  
Sun Ok Hwang ◽  
...  
Keyword(s):  
Cho Cells ◽  
Recombinant Cho Cells

Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress

2005 ◽  
pp. 75-78
Author(s):  
Moon Sue Lee ◽  
Kyoung Wook Kim ◽  
Young Hwan Kim ◽  
Gyun Min Lee
Keyword(s):  
Cho Cells ◽  
Proteome Analysis ◽  
Hyperosmotic Stress ◽  
Recombinant Cho Cells
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