Early morphologic changes in coronary arteries of experimental diabetic rats

Diabetes mellitus was induced in rats with streptozotocin and after 3 months the animals (n = 48) received an i.v. injection of 1 or 3 g I/kg in the form of high-osmolar diatrizoate, low-osmolar iopromide or iohexol, or of 0.6 g I/kg of high-osmolar Gd-DTPA. The controls were given an i.v. injection of physiologic saline. After 2 hours the kidneys were fixed by perfusion and the renal morphologic changes were semiquantitatively analyzed by two independent observers unaware of the agent administered. The contrast media (CM) induced pronounced cytoplasmic vacuolization in the proximal convoluted tubular cells. Such a lysosomal alteration may indicate CM uptake into the cell, and the ultrastructural evaluation revealed intracellular injuries related to the process. The alterations were most marked following administration of iohexol, but diatrizoate also induced a statistically highly significant vacuolization (p < 0.001). The lysosomal alterations following iopromide administration were not as striking, and Gd-DTPA induced only minor changes.

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An Overview of Human Arterial Pathology

Toxicologic Pathology ◽

10.1177/019262338901700118 ◽

1989 ◽

Vol 17 (1_part_2) ◽

pp. 219-231 ◽

Cited By ~ 4

Author(s):

Hugh A. Mcallister

Keyword(s):

Blood Vessels ◽

Coronary Arteries ◽

Arterial Disease ◽

Peripheral Arteries ◽

Dissecting Aneurysms ◽

Morphologic Changes

In this manuscript the pathology of human arterial disease, including diseases of the aorta, coronary arteries, and peripheral arteries, is reviewed. Common atherosclerotic-related lesions and varied forms of aortitis, arteritis, and dissecting aneurysms are described in terms of their gross and microscopic characteristics. In addition, rarer occurrences, such as congenital hypoplasia of the arteries, are mentioned. Finally, morphologic changes in blood vessels that have been affected by drugs or toxins are summarized.

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Immediate and Long-term Morphologic Changes in Stenosis Geometry after Wiktor™ Stent Implantation in Native Coronary Arteries for Recurrent Stenosis Following Balloon Angioplasty. Report on the First Fifty Consecutive Patients

Restenosis after Intervention with New Mechanical Devices - Developments in Cardiovascular Medicine ◽

10.1007/978-94-011-2650-2_13 ◽

1992 ◽

pp. 215-234 ◽

Cited By ~ 2

Author(s):

Peter de Jaegere ◽

Patrick W. Serruys ◽

Willem van der Giessen ◽

Pim de Feyter

Keyword(s):

Coronary Arteries ◽

Balloon Angioplasty ◽

Stent Implantation ◽

Recurrent Stenosis ◽

Morphologic Changes

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Morphologic Changes in Coronary Arteries of Children in Different Geographic Locations

Atherosclerosis VI ◽

10.1007/978-3-642-81817-2_12 ◽

1983 ◽

pp. 77-81

Author(s):

R. F. Scott ◽

A. S. Daoud

Keyword(s):

Coronary Arteries ◽

Geographic Locations ◽

Morphologic Changes

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Early and late morphologic changes in major epicardial coronary arteries after percutaneous transluminal coronary angioplasty

The American Journal of Cardiology ◽

10.1016/0002-9149(84)90744-6 ◽

1984 ◽

Vol 53 (12) ◽

pp. C42-C47 ◽

Cited By ~ 106

Author(s):

Bruce F. Waller ◽

H.Joel Gorfinkel ◽

Felix J. Rogers ◽

Kenneth M. Kent ◽

William C. Roberts

Keyword(s):

Coronary Arteries ◽

Coronary Angioplasty ◽

Percutaneous Transluminal Coronary Angioplasty ◽

Transluminal Coronary Angioplasty ◽

Percutaneous Transluminal ◽

Morphologic Changes

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Ebselen reduces nitration and restores voltage-gated potassium channel function in small coronary arteries of diabetic rats

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00717.2007 ◽

2007 ◽

Vol 293 (4) ◽

pp. H2231-H2237 ◽

Cited By ~ 25

Author(s):

Aaron H. Bubolz ◽

Qingping Wu ◽

Brandon T. Larsen ◽

David D. Gutterman ◽

Yanping Liu

Keyword(s):

Diabetes Mellitus ◽

Smooth Muscle ◽

Smooth Muscle Cells ◽

Coronary Arteries ◽

Nitrosative Stress ◽

Muscle Cells ◽

Diabetic Rats ◽

Causative Role ◽

Α Subunit ◽

Channel Function

Small coronary arteries (SCA) from diabetic rats exhibit enhanced peroxynitrite (ONOO−) formation and concurrent impairment of voltage-dependent potassium (Kv) channel function. However, it is unclear whether ONOO− plays a causative role in this impairment. We hypothesized that functional loss of Kv channels in coronary smooth muscle cells (SMC) in diabetes is due to ONOO− with subsequent tyrosine nitration of Kv channel proteins. Diabetic rats and nondiabetic controls were treated with or without ebselen (Eb) for 4 wk. SCA were prepared for immunohistochemistry (IHC), immunoprecipitation (IP) followed by Western blot (WB), videomicroscopy, and patch-clamp analysis. IHC revealed excess ONOO− in SCA from diabetic rats. IP and WB revealed elevated nitration of the Kv1.2 α-subunit and reduced Kv1.2 protein expression in diabetic rats. Each of these changes was improved in Eb-treated rats. Protein nitration and Kv1.5 expression were unchanged in SCA from diabetic rats. Forskolin, a direct cAMP activator that induces Kv1 channel activity, dilated SCA from nondiabetic rats in a correolide (Cor; a selective Kv1 channel blocker)-sensitive fashion. Cor did not alter the reduced dilation to forskolin in diabetic rats; however, Eb partially restored the Cor-sensitive component of dilation. Basal Kv current density and response to forskolin were improved in smooth muscle cells from Eb-treated DM rats. We conclude that enhanced nitrosative stress in diabetes mellitus contributes to Kv1 channel dysfunction in the coronary microcirculation. Eb may be beneficial for the therapeutic treatment of vascular complications in diabetes mellitus.

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