Hormonal induction of functional differentiation and mammary-derived growth inhibitor expression in cultured mouse mammary gland explants

1992 ◽  
Vol 28 (9-10) ◽  
pp. 625-634 ◽  
Author(s):  
B. Binas ◽  
E. Spitzer ◽  
W. Zschiesche ◽  
B. Erdmann ◽  
A. Kurtz ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Growth Inhibitor ◽  
Functional Differentiation ◽  
Mouse Mammary Gland ◽  
Hormonal Induction

scholarly journals C/EBPbeta , but not C/EBPalpha , is essential for ductal morphogenesis, lobuloalveolar proliferation, and functional differentiation in the mouse mammary gland

1998 ◽  
Vol 12 (12) ◽  
pp. 1917-1928 ◽  
Author(s):  
T. N. Seagroves ◽  
S. Krnacik ◽  
B. Raught ◽  
J. Gay ◽  
B. Burgess-Beusse ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Functional Differentiation ◽  
Mouse Mammary Gland ◽  
Ductal Morphogenesis

DNA synthesis in the absence of cell reproduction during functional differentiation of mouse mammary gland

Life Sciences ◽  
1971 ◽  
Vol 10 (15) ◽  
pp. 867-877 ◽  
Author(s):  
M.R. Banerjee ◽  
Janice E. Wagner ◽  
Denva L. Kinder
Keyword(s):  
Mammary Gland ◽  
Dna Synthesis ◽  
Functional Differentiation ◽  
Mouse Mammary Gland ◽  
Cell Reproduction

Involvement of autocrine mechanism of transforming growth factor-β in the functional differentiation of pregnant mouse mammary gland

1994 ◽  
Vol 130 (3) ◽  
pp. 302-307 ◽  
Author(s):  
Toshiya Yamamoto ◽  
Hiroko Komura ◽  
Ken-ichirou Morishige ◽  
Chikae Tadokoro ◽  
Masahiro Sakata ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Mammary Epithelial Cells ◽  
Transforming Growth Factor Β ◽  
Functional Differentiation ◽  
Pregnant Mouse ◽  
Mouse Mammary Gland ◽  
Autocrine Mechanism

Yamamoto T, Komura H, Morishige K, Tadokoro C, Sakata M, Kurachi H, Miyake A. Involvement of autocrine mechanism of transforming growth factor-β in the functional differentiation of pregnant mouse mammary gland. Eur J Endocrinol 1994;130:302–7. ISSN 0804–4643 We studied the presence and possible role of the autocrine mechanism of transforming growth factor-β (TGF-β) in pregnant mouse mammary gland. Northern blot analysis revealed the expression of the TGF-β1 gene transcript at 2.5 kb in mammary epithelial cells isolated from virgin and mid-pregnant mice. The TGF-β activity was higher in the conditioned medium from mid-pregnant mouse mammary explants than that from virgin explants by a bioassay system using mink lung epithelial cells. A binding study using [125I]TGF-β1 as a ligand showed that pregnant mouse mammary epithelial cells possessed a single class of high-affinity TGF-β1 binding sites (Kd = 28.0 pmol/l, 1.2 × 104 sites per cell). These results suggested the presence of a TGF-β autocrine mechanism in pregnant mouse mammary epithelial cells. Next, we examined the effect of TGF-β1 on the functional differentiation of pregnant mouse mammary gland. Transforming growth factor-β1 inhibited α-lactalbumin production in cultured mammary explants from mid-pregnant mice in a dose-dependent manner without inhibiting DNA synthesis. All these results suggest that TGF-β may play a role in regulating the functional differentiation of mouse mammary glands during pregnancy. Hirohisa Kurachi, Department of Obstetrics and Gynecology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565, Japan

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

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