Coat protein sequence shows thatCucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II

2006 ◽  
Vol 31 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Neeraj Verma ◽  
B. K. Mahinghara ◽  
Raja Ram ◽  
A. A. Zaidi
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Protein Sequence ◽  
Virus Isolate ◽  
Coat Protein Sequence ◽  
Subgroup Ii

Partial sequencing of the genomic RNA of Araujia mosaic virus and comparison of the coat protein sequence with those of other potyviruses

2007 ◽  
Vol 152 (11) ◽  
pp. 2125-2129 ◽  
Author(s):  
B. Massey ◽  
X. Cui ◽  
E. Hiebert ◽  
M. S. Elliott ◽  
N. Waipara ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Protein Sequence ◽  
Coat Protein Sequence ◽  
Genomic Rna ◽  
Partial Sequencing

scholarly journals First Report of Cucumber mosaic virus Subgroup II Infecting Lycopersicon esculentum in India

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1457-1457 ◽  
Author(s):  
N. Sudhakar ◽  
D. Nagendra-Prasad ◽  
N. Mohan ◽  
K. Murugesan
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tamil Nadu ◽  
Indian Subcontinent ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Leaf ◽  
Polymorphism Analysis ◽  
First Report ◽  
Subgroup Ii

During a survey in January 2006 near Salem in Tamil Nadu (south India), Cucumber mosaic virus was observed infecting tomatoes with an incidence of more than 70%. Plants exhibiting severe mosaic, leaf puckering, and stunted growth were collected, and the virus was identified using diagnostic hosts, evaluation of physical properties of the virus, compound enzyme-linked immunosorbent assay (ELISA) (ELISA Lab, Washington State University, Prosser), reverse-transcription polymerase chain reaction (RT-PCR), and restriction fragment length polymorphism analysis (DSMZ, S. Winter, Germany). To determine the specific CMV subgroup, total RNA was extracted from 50 infected leaf samples using the RNeasy plant RNA isolation kit (Qiagen, Hilden, Germany) and tested for the presence of the complete CMV coat protein gene using specific primers as described by Rizos et al. (1). A fragment of the coat protein was amplified and subsequently digested with MspI to reveal a pattern of two fragments (336 and 538 bp), indicating CMV subgroup II. No evidence of mixed infection with CMV subgroup I was obtained when CMV isolates representing subgroups I (PV-0419) and II (PV-0420), available at the DSMZ Plant Virus Collection, were used as controls. Only CMV subgroup I has been found to predominantly infect tomato in the Indian subcontinent, although Verma et al. (2) identified CMV subgroup II infecting Pelargonium spp., an ornamental plant. To our knowledge, this is the first report of CMV subgroup II infecting tomato crops in India. References: (1) H. Rizos et al. J. Gen. Virol. 73:2099, 1992. (2) N. Verma et al. J. Biol. Sci. 31:47, 2006.

Preparation of polyclonal antibody against recombinant coat protein of Cucumber mosaic virus isolate B13

2017 ◽  
Vol 6 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Davoud Koolivand ◽  
◽  
Nemat Sokhandan Bashir ◽  
Afshin Rostami ◽  
◽  
...  
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Polyclonal Antibody ◽  
Virus Isolate ◽  
Cucumber Mosaic Virus Isolate ◽  
Recombinant Coat Protein
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