On the study of proteolytic enzyme activities in the plasma and gastric juice of the patients with peptic ulcer and gastric cancer

Gastric cancer is a multifactorial disease with complex interplay of environmental and genetic factors. Helicobacter pylori (H. pylori) infestation has been identified as the most important etiological agent in the pathogenesis of gastric cancer. Also, the role of dietary factors that is low consumption of fruits and vegetables have been found to be associated with gastric cancer. Among the dietary factors, antioxidants especially vitamin C has been found to confer the strongest protection against gastric cancer. Its anti-proliferative and pro-apoptotic action has been suggested in vitro. Because of its antioxidant activity, it protects cells against oxidative DNA damage caused by toxic effects of reactive oxygen species. It also inhibits production of carcinogenic N-nitroso compound in the stomach. The person with H. pylori infection has low levels of vitamin C in their gastric juice and levels of vitamin C normalizes on eradication of H. pylori. Vitamin C levels are high in gastric mucosa and gastric juice, sometimes more than that of in plasma. But gastric pathological conditions cause lowered secretion of vitamin C into gastric juice. Effect of H. pylori on vitamin C in gastric juice is reversible and on eradication of H. pylori, it returns to normal level. Hence, eradication of H. pylori and chemoprevention with antioxidant supplementation will be an effective preventive strategy to reduce the incidence of gastric cancer and related mortality. Vitamin C and gastric cancer is an area of potential interest for researchers as a preventive measure. Keywords: Vitamin C, H. pylori, gastric cancer.

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Association BetweenPorphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitansand Spirochaetes with Neutral Proteolytic Enzyme Activities in Deep Periodontal Pockets

Microbial Ecology in Health and Disease ◽

10.3402/mehd.v6i2.8095 ◽

1993 ◽

Vol 6 (2) ◽

Author(s):

M. Mäkelä ◽

E. Söderling ◽

K. Paunio

Keyword(s):

Enzyme Activities ◽

Proteolytic Enzyme ◽

Prevotella Intermedia

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Use of cimetidine and other peptic ulcer drugs in Denmark 1977-1990 with analysis of the risk of gastric cancer among cimetidine users.

Gut ◽

10.1136/gut.33.9.1166 ◽

1992 ◽

Vol 33 (9) ◽

pp. 1166-1169 ◽

Cited By ~ 21

Author(s):

H Moller ◽

A Nissen ◽

J Mosbech

Keyword(s):

Gastric Cancer ◽

Peptic Ulcer

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Associations between cytokine gene polymorphisms and susceptibility to Helicobacter pylori infection and Helicobacter pylori related gastric cancer, peptic ulcer disease: A meta-analysis

PLoS ONE ◽

10.1371/journal.pone.0176463 ◽

2017 ◽

Vol 12 (4) ◽

pp. e0176463 ◽

Cited By ~ 13

Author(s):

Jingjing Ma ◽

Dandan Wu ◽

Xue Hu ◽

Jiao Li ◽

Mingwei Cao ◽

...

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Peptic Ulcer Disease ◽

Gene Polymorphisms ◽

Meta Analysis ◽

Helicobacter Pylori Infection ◽

Cytokine Gene ◽

Ulcer Disease ◽

Cytokine Gene Polymorphisms

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The Intriguing Relationship of Helicobacter pylori Infection and Acid Secretion in Peptic Ulcer Disease and Gastric Cancer

Digestive Diseases ◽

10.1159/000332213 ◽

2011 ◽

Vol 29 (5) ◽

pp. 459-464 ◽

Cited By ~ 48

Author(s):

P. Malfertheiner

Keyword(s):

Gastric Cancer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Acid Secretion ◽

Peptic Ulcer Disease ◽

Ulcer Disease ◽

Relationship Of

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Helicobacter pyloriAnti-CagA Antibodies: Prevalence in Symptomatic and Asymptomatic Subjects in Turkey

Canadian Journal of Gastroenterology ◽

10.1155/2002/589087 ◽

2002 ◽

Vol 16 (8) ◽

pp. 527-532 ◽

Cited By ~ 13

Author(s):

M Fatih Abasiyanik ◽

Ersan Sander ◽

Barik A Salih

Keyword(s):

Gastric Cancer ◽

Duodenal Ulcer ◽

Helicobacter Pylori ◽

Peptic Ulcer ◽

Chronic Gastritis ◽

Molecular Weights ◽

Cancer Antigens ◽

Gastroduodenal Disease ◽

H Pylori ◽

Asymptomatic Subjects

BACKGROUND: Several reports have shown the prevalence of anti-CagA antibodies to be associated with the development of peptic ulcer diseases, while others have indicated that there is no such association.AIM: To examine the prevalence of antibodies to CagA and otherHelicobacter pyloriantigens in symptomatic and asymptomatic subjects in Turkey.SUBJECTS AND METHODS: Sixty-six symptomatic subjects, 16 to 74 years of age, were examined forH pyloriby biopsy-based tests and ELISA. One hundred nineteen asymptomatic subjects, 20 to 65 years of age, were also tested serologically for the presence ofH pylori. Samples from both groups that were found to be positive forH pyloriby ELISA were then tested by immunoblotting.RESULTS: Fifty-four (82%) symptomatic subjects and 76 (64%) asymptomatic subjects were found to beH pylori-positive by ELISA. Samples from 30 symptomatic subjects who were found to beH pylori-positive by ELISA were analyzed by immunoblotting. Antibodies to CagA (116 kDa) antigen were detected in immunoblots of 11 of 14 (79%) with chronic gastritis, 12 of 13 (92%) with duodenal ulcer and three of three (100%) with gastric cancer. Antigens of the following molecular weights were also detected in these 30 subjects: 89 kDa (VacA) in 21 (70%), 37 kDa in 21 (70%), 35 kDa in 19 (63%), 30 kDa in 27 (90%) and 19.5 kDa in 19 (63%). Immunoblots of 40 ELISA-positive asymptomatic subjects showed that 33 (83%) had antibodies to CagA antigen, 26 (65%) to VacA antigen, 30 (75%) to a 37 kDa antigen, 30 (75%) to a 35 kDa antigen, 39 (98%) to a 30 kDa antigen and 36 (90%) to a 19.5 kDa antigen.CONCLUSIONS: Antibodies to CagA antigen were prevalent in both groups, regardless of the presence of gastroduodenal disease.

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