Specific affinity for phosphate uptake and specific alkaline phosphatase activity as diagnostic tools for detecting phosphorus-limited phytoplankton and bacteria

2006 ◽  
Vol 29 (6) ◽  
pp. 1226-1241 ◽  
Author(s):  
Tsuneo Tanaka ◽  
Peter Henriksen ◽  
Risto Lignell ◽  
Kalle Olli ◽  
Jukka Seppälä ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Phosphate Uptake ◽  
Diagnostic Tools ◽  
Specific Alkaline Phosphatase ◽  
Specific Affinity

Histochemical Distribution of 5-Nucleotidase in Snake Tissues: Presence of 5-Nucleotidase and Absence of Alkaline Phosphomonoesterase in Venom Glands of the Rattlesnake

1952 ◽  
Vol s3-93 (24) ◽  
pp. 391-394
Author(s):  
D. E. BRAGDON ◽  
J.F. A. MCMANUS
Keyword(s):  
Alkaline Phosphatase ◽  
Smooth Muscle ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Venom Gland ◽  
Specific Alkaline Phosphatase ◽  
Rattlesnake Venom ◽  
Great Vessels ◽  
Venom Glands ◽  
Thyroid Epithelium

1. Activity of the specific alkaline phosphatase, 5-nucleotidase, is intense in the epithelium and secretion of the rattlesnake venom gland. Non-specific alkaline phosphatase activity is lacking. 2. Thyroid epithelium, the smooth muscle of great vessels, and (inconstantly) smooth muscle of abdominal hollow viscera show greater 5-nucleotidase than nonspecific activity. 3. These findings confirm the specificity of 5-nucleotidase.

Intestinal phosphate transport and alkaline phosphatase activity in the chick

1981 ◽  
Vol 240 (4) ◽  
pp. E384-E390 ◽  
Author(s):  
S. J. Birge ◽  
R. C. Avioli
Keyword(s):  
Alkaline Phosphatase ◽  
Epithelial Cells ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Phosphate Uptake ◽  
Intestinal Epithelial Cells ◽  
Phosphate Transport ◽  
Intestinal Epithelial ◽  
Ph Optimum

The initial rates of phosphate accumulation by isolated chick intestinal epithelial cells have been examined. At high concentrations of phosphate (1.5 mM), phosphate uptake is relatively independent of sodium and demonstrates a pH optimum of 8.0. At pH 8.0, 56% of the uptake is dependent on the presence of Ca in the uptake medium compared to 28% at pH 6.8. Membranes prepared from these same intestinal epithelial cells contain a Ca-dependent phosphatase that can be distinguished from the more abundant Mg-dependent alkaline phosphatase. The Ca-dependent phosphatase has a pH optimum between 8.5 and 9.0 and, compared to the Mg-dependent activity, is more readily inactivated at 58 degrees C and is relatively resistant to L-phenylalanine inhibition but more sensitive to ethane-1-hydroxy-1,1-diphosphonate (EHDP). Both activities are distributed in a constant proportion between the brush border and basal lateral membranes and at various segments along the intestine. Vitamin D in vivo and 25-hydroxyvitamin D [25(OH)D] in vitro stimulated both activities. In vitro, utilizing the isolated intestinal cells, the stimulation of phosphate uptake paralleled the increase in Ca-dependent alkaline phosphatase activity. The role of alkaline phosphatase in intestinal phosphate transport is discussed.

Serum Osteocalcin, Total and Bone-Specific Alkaline Phosphatase following Isolated Tibial Shaft Fracture

1996 ◽  
Vol 33 (3) ◽  
pp. 196-200 ◽  
Author(s):  
Shirley A Bowles ◽  
Nazeer Kurdy ◽  
Alison M Davis ◽  
Michael W France ◽  
David R Marsh
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Healing Process ◽  
Shaft Fracture ◽  
Tibial Shaft Fracture ◽  
Tibial Shaft ◽  
Post Injury ◽  
Specific Alkaline Phosphatase ◽  
Bone Specific Alkaline Phosphatase

We report the changes in osteocalcin concentrations and in total and bone specific alkaline phosphatase activity occurring in the twenty week period following tibial shaft fracture in 20 subjects. Bone formation during the healing process is reflected by progressive increases in the concentration of osteocalcin and bone specific alkaline phosphatase after week 5 and the latter correlated with the height and weight of the subject. In the early post injury period, total alkaline phosphatase activity increased whereas that of the bone isoenzyme initially fell, starting to rise again during the second week. After an immediate post injury rise, osteocalcin concentration also decreased, reaching a nadir by week 5. As only three of our subjects demonstrated delayed union, we have not been able to demonstrate that biochemical monitoring of the healing process can provide an indication of prognosis in tibial shaft fracture.

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