Immobilization of glucose isomerase and its application in continuous production of high fructose syrup

1998 ◽  
Vol 69 (3) ◽  
pp. 203-215 ◽  
Author(s):  
Yubin Ge ◽  
Hui Zhou ◽  
Wei Kong ◽  
Yi Tong ◽  
Shuyan Wang ◽  
...  
Keyword(s):  
Continuous Production ◽  
Glucose Isomerase ◽  
High Fructose ◽  
High Fructose Syrup

Immobilization of glucose isomerase and its application in continuous production of high fructose syrup

1998 ◽  
Vol 69 (1) ◽  
pp. 17-29 ◽  
Author(s):  
Yubin Ge ◽  
Hui Zhou ◽  
Wei Kong ◽  
Yi Tong ◽  
Shuyan Wang ◽  
...  
Keyword(s):  
Continuous Production ◽  
Glucose Isomerase ◽  
High Fructose ◽  
High Fructose Syrup

scholarly journals Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor

2020 ◽  
Vol 309 ◽  
pp. 125710 ◽  
Author(s):  
Sawssan Neifar ◽  
Fadia V. Cervantes ◽  
Amel Bouanane-Darenfed ◽  
Hajer BenHlima ◽  
Antonio O. Ballesteros ◽  
...  
Keyword(s):  
Packed Bed ◽  
Glucose Isomerase ◽  
Packed Bed Reactor ◽  
High Fructose ◽  
High Fructose Syrup

Production of High Fructose Syrup by Glucose Isomerase Immobilized on Phenol-Formaldehyde Resin

1975 ◽  
Vol 27 (9) ◽  
pp. 302-306 ◽  
Author(s):  
Y. Yokote ◽  
K. Kimura ◽  
H. Samejima
Keyword(s):  
Phenol Formaldehyde ◽  
Glucose Isomerase ◽  
Phenol Formaldehyde Resin ◽  
Formaldehyde Resin ◽  
High Fructose ◽  
High Fructose Syrup

scholarly journals Glucose isomerization in simulated moving bed reactor by Glucose isomerase

2006 ◽  
Vol 49 (3) ◽  
pp. 491-502 ◽  
Author(s):  
Eduardo Alberto Borges da Silva ◽  
Antônio Augusto Ulson de Souza ◽  
Alírio Egídio Rodrigues ◽  
Selene Maria Arruda Guelli Ulson de Souza
Keyword(s):  
Flow Rate ◽  
Enzymatic Reaction ◽  
Glucose Isomerase ◽  
Simulated Moving Bed ◽  
Reactive System ◽  
Moving Bed ◽  
High Fructose ◽  
And Performance ◽  
High Fructose Syrup ◽  
Performance Results

Studies were carried out on the production of high-fructose syrup by Simulated Moving Bed (SMB) technology. A mathematical model and numerical methodology were used to predict the behavior and performance of the simulated moving bed reactors and to verify some important aspects for application of this technology in the isomerization process. The developed algorithm used the strategy that considered equivalences between simulated moving bed reactors and true moving bed reactors. The kinetic parameters of the enzymatic reaction were obtained experimentally using discontinuous reactors by the Lineweaver-Burk technique. Mass transfer effects in the reaction conversion using the immobilized enzyme glucose isomerase were investigated. In the SMB reactive system, the operational variable flow rate of feed stream was evaluated to determine its influence on system performance. Results showed that there were some flow rate values at which greater purities could be obtained.

scholarly journals Integrative Gene Cloning and Expression System forStreptomycessp. US 24 andStreptomycessp. TN 58 Bioactive Molecule Producing Strains

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Samiha Sioud ◽  
Bertrand Aigle ◽  
Ines Karray-Rebai ◽  
Slim Smaoui ◽  
Samir Bejar ◽  
...  
Keyword(s):  
Expression System ◽  
Morphological Differentiation ◽  
Single Copy ◽  
Glucose Isomerase ◽  
Bioactive Molecules ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Isomerase Activity ◽  
High Fructose ◽  
High Fructose Syrup

Streptomycessp. US 24 andStreptomycessp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed usingE. coliET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For theStreptomycessp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas forStreptomycessp. TN 58, the integration was in single copy at theattBsite. Plasmid pSET152 was inherited every time for all analysedStreptomycessp. US 24 andStreptomycessp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) fromStreptomycessp. SK was expressed in strainStreptomycessp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of theStreptomycessp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

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