Preparation and properties of mycelium-bound glucose isomerase co-immobilized with glucoamylase, and continuous production of high fructose-syrup directly from liquefied starch.

Studies were carried out on the production of high-fructose syrup by Simulated Moving Bed (SMB) technology. A mathematical model and numerical methodology were used to predict the behavior and performance of the simulated moving bed reactors and to verify some important aspects for application of this technology in the isomerization process. The developed algorithm used the strategy that considered equivalences between simulated moving bed reactors and true moving bed reactors. The kinetic parameters of the enzymatic reaction were obtained experimentally using discontinuous reactors by the Lineweaver-Burk technique. Mass transfer effects in the reaction conversion using the immobilized enzyme glucose isomerase were investigated. In the SMB reactive system, the operational variable flow rate of feed stream was evaluated to determine its influence on system performance. Results showed that there were some flow rate values at which greater purities could be obtained.

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Isomerization of glucose to fructose. 2. Optimization of reaction conditions in the production of high fructose syrup by isomerization of glucose catalyzed by a whole cell immobilized glucose isomerase catalyst

Industrial & Engineering Chemistry Process Design and Development ◽

10.1021/i200022a003 ◽

1983 ◽

Vol 22 (3) ◽

pp. 356-361 ◽

Cited By ~ 13

Author(s):

J. Straatsma ◽

K. Vellenga ◽

H. G. J. De Wilt ◽

G. E. H. Joosten

Keyword(s):

Glucose Isomerase ◽

Reaction Conditions ◽

Whole Cell ◽

High Fructose ◽

Fructose 2 ◽

High Fructose Syrup ◽

Immobilized Glucose Isomerase

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Integrative Gene Cloning and Expression System forStreptomycessp. US 24 andStreptomycessp. TN 58 Bioactive Molecule Producing Strains

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/464986 ◽

2009 ◽

Vol 2009 ◽

pp. 1-10 ◽

Cited By ~ 6

Author(s):

Samiha Sioud ◽

Bertrand Aigle ◽

Ines Karray-Rebai ◽

Slim Smaoui ◽

Samir Bejar ◽

...

Keyword(s):

Expression System ◽

Morphological Differentiation ◽

Single Copy ◽

Glucose Isomerase ◽

Bioactive Molecules ◽

Cloning And Expression ◽

Gene Cloning And Expression ◽

Isomerase Activity ◽

High Fructose ◽

High Fructose Syrup

Streptomycessp. US 24 andStreptomycessp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed usingE. coliET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For theStreptomycessp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas forStreptomycessp. TN 58, the integration was in single copy at theattBsite. Plasmid pSET152 was inherited every time for all analysedStreptomycessp. US 24 andStreptomycessp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) fromStreptomycessp. SK was expressed in strainStreptomycessp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of theStreptomycessp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.

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