Purification and Characterization of a Milk Clotting Protease fromMucor bacilliformis

1992 ◽  
Vol 37 (3) ◽  
pp. 283-294 ◽  
Author(s):  
Liliana B. Areces ◽  
Mirtha Biscoglio De Jiménez Bonino ◽  
Marina A. A. Parry ◽  
Elda R. Fraile ◽  
Héctor M. Fernández ◽  
...  
Keyword(s):  
Purification And Characterization ◽  
Milk Clotting ◽  
Milk Clotting Protease

scholarly journals Purification and characterization of a milk-clotting protease from Bacillus licheniformis strain USC13

2007 ◽  
Vol 103 (6) ◽  
pp. 2205-2213 ◽  
Author(s):  
J.M. Ageitos ◽  
J.A. Vallejo ◽  
A.B.F. Sestelo ◽  
M. Poza ◽  
T.G. Villa
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization ◽  
Milk Clotting ◽  
Milk Clotting Protease

scholarly journals Production and characterization of a milk-clotting protease in the crude enzymatic extract from the newly isolated Thermomucor indicae-seudaticae N31

2010 ◽  
Vol 120 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Carolina Merheb-Dini ◽  
Eleni Gomes ◽  
Maurício Boscolo ◽  
Roberto da Silva
Keyword(s):  
Enzymatic Extract ◽  
Milk Clotting ◽  
Milk Clotting Protease

scholarly journals Purification and Characterization of Milk Clotting Enzyme from Artichoke (Cynara cardunculus L.) Flowers as Coagulant on White Soft Cheese

2017 ◽  
Vol 12 (4) ◽  
pp. 254-265 ◽  
Author(s):  
Baraka Abo El-Yazeed Abd El-Sal ◽  
Osama Abd El-Hamid Ibrahim ◽  
Howida Abd El-Razek El-Sayed
Keyword(s):  
Cynara Cardunculus ◽  
Purification And Characterization ◽  
Milk Clotting ◽  
Soft Cheese ◽  
Milk Clotting Enzyme

scholarly journals PRODUCTION AND PARTIAL CHARACTERIZATION OF A MILK-CLOTTING PROTEINASE PRODUCED BY BACILLUS SUBTILIS SMDFS-2B MN715837 IN SUBMERGED CULTURES

2021 ◽  
Vol 4 (2) ◽  
pp. e257
Author(s):  
Jermen Mamo ◽  
Martin Kangwa ◽  
Hector-Marcelo Fernandez-Lahore ◽  
Fassil Assefa
Keyword(s):  
Bacillus Subtilis ◽  
Crude Enzyme ◽  
Partial Characterization ◽  
Effect Of Temperature ◽  
Submerged Cultures ◽  
Milk Clotting ◽  
Milk Clotting Protease ◽  
Pepstatin A ◽  
Milk Clotting Activity

This study focused on the production and partial characterization of a milk-clotting protease produced by Bacillus subtilis SMDFS 2B in submerged cultures, under partially optimized conditions. The crude enzyme was recovered in the culture supernatant and concentrate was produced after cell removal and subsequent dialysis. Inhibition studies were conducted employing four distinct protease inhibitors: Pepstatin-A, Phenylmethane-sulphonyl-fluoride (PMSF), Ethylenediaminetetraacetic acid (EDTA), and iodoacetamide (IA). The effect of temperature, pH, metal ions and substrate concentration on milk-clotting activity were also evaluated. The thermal stability of the enzyme was determined by incubating the crude enzyme at a temperature value ranging from 35 oC to 60 oC. Similarly, pH stability was determined at pH values ranging between 4.5 and 8.0. The highest milk-clotting activity was observed at a temperature of 55 oC and pH 5.5. The crude enzyme preparation remained stable on incubation at 35 oC and 40 oC for 15 min and at pH 5.5. The enzyme also showed the lowest residual milk-clotting activity in the presence of EDTA (7.94%) and Pepstatin-A (26.71%). The addition of Mg2+ and Mn2+ significantly increased milk-clotting activity. The enzyme also showed an elevation in its apparent milk-clotting activity upon increasing the substrate (skim-milk) concentration. Thus, the milk-clotting protease produced by B. subtilis SMDFS 2B by submerged fermentation revealed some interesting milk-clotting characteristics. This may open the way for applications in the food and dairy industries.

Production and characterization of the milk-clotting protease of Myxococcus xanthus strain 422

2003 ◽  
Vol 30 (12) ◽  
pp. 691-698 ◽  
Author(s):  
M. Poza ◽  
C. Sieiro ◽  
L. Carreira ◽  
J. Barros-Vel�zquez ◽  
T. G. Villa
Keyword(s):  
Myxococcus Xanthus ◽  
Milk Clotting ◽  
Milk Clotting Protease

Purification and characterization of milk clotting enzyme from goat (Capra hircus)

2006 ◽  
Vol 145 (1) ◽  
pp. 108-113 ◽  
Author(s):  
Ashwani Kumar ◽  
Jitender Sharma ◽  
Ashok Kumar Mohanty ◽  
Sunita Grover ◽  
Virender Kumar Batish
Keyword(s):  
Capra Hircus ◽  
Purification And Characterization ◽  
Milk Clotting ◽  
Milk Clotting Enzyme
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