Intermediate filament expression in normal and vitamin A depleted cultured hamster tracheal epithelium as detected by monoclonal antibodies

1988 ◽  
Vol 56 (1) ◽  
pp. 103-110 ◽  
Author(s):  
A. A. J. J. L. Rutten ◽  
J. P. Bruyntjes ◽  
F. C. S. Ramaekers
Keyword(s):  
Monoclonal Antibodies ◽  
Vitamin A ◽  
Intermediate Filament ◽  
Tracheal Epithelium

Vitamin A and β-carotene influence the level of benzo[a]pyrene-induced DNA adducts and DNA-repair activities in hamster tracheal epithelium in organ culture

1995 ◽  
Vol 91 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Andreas P.M. Wolterbeek ◽  
Robert Roggeband ◽  
Catherina J.A. van Moorsel ◽  
Robert A. Baan ◽  
Jan H. Koeman ◽  
...  
Keyword(s):  
Dna Repair ◽  
Vitamin A ◽  
Organ Culture ◽  
Dna Adducts ◽  
Tracheal Epithelium ◽  
Β Carotene

Differential diagnosis of genitourinary tumors using monoclonal antibodies to intermediate filament proteins

Urology ◽  
1989 ◽  
Vol 33 (5) ◽  
pp. 433-439
Author(s):  
Steven A. Lofton ◽  
Allen M. Gown ◽  
Arthur M. Vogel ◽  
John N. Krieger
Keyword(s):  
Differential Diagnosis ◽  
Monoclonal Antibodies ◽  
Intermediate Filament ◽  
Intermediate Filament Proteins

Surface Morphology of Tracheal Epithelium in Vitamin A Deficiency and Reversal

1974 ◽  
pp. 257-264 ◽  
Author(s):  
Curtis D. Port ◽  
David W. Baxter ◽  
Curtis C. Harris
Keyword(s):  
Vitamin A ◽  
Surface Morphology ◽  
Vitamin A Deficiency ◽  
Tracheal Epithelium

scholarly journals Monoclonal antibodies to desmin, the muscle-specific intermediate filament protein.

1983 ◽  
Vol 2 (12) ◽  
pp. 2305-2312 ◽  
Author(s):  
E. Debus ◽  
K. Weber ◽  
M. Osborn
Keyword(s):  
Monoclonal Antibodies ◽  
Intermediate Filament ◽  
Intermediate Filament Protein ◽  
Filament Protein

scholarly journals Two Drosophila melanogaster proteins related to intermediate filament proteins of vertebrate cells.

1981 ◽  
Vol 91 (1) ◽  
pp. 175-183 ◽  
Author(s):  
F G Falkner ◽  
H Saumweber ◽  
H Biessmann
Keyword(s):  
Drosophila Melanogaster ◽  
Monoclonal Antibodies ◽  
Heat Shock ◽  
Indirect Immunofluorescence ◽  
Intermediate Filament ◽  
Cross Reaction ◽  
Cytoplasmic Protein ◽  
Baby Hamster Kidney ◽  
Intermediate Filament Proteins ◽  
Bhk Cells

Monoclonal antibodies were prepared against a 46,000 mol wt major cytoplasmic protein from Drosophila melanogaster Kc cells. These antibodies reacted with the 46,000 and a 40,000 mol wt protein from Kc cells. Some antibodies showed cross-reaction with 55,000 (vimentin) and 52,000 mol wt (desmin) proteins from baby hamster kidney (BHK) cells that form intermediate sized filaments in vertebrate cells. In indirect immunofluorescence, the group of cross reacting antibodies stained a filamentous meshwork in the cytoplasm of vertebrate cells. In Kc cells the fluorescence seemed to be localized in a filamentous meshwork that became more obvious after the cells had flattened out on a surface. These cytoskeletal structures are heat-labile; the proteins in Kc or BHK cells rearrange after a brief heat shock, forming juxtanuclear cap structures.

scholarly journals Clathrin assembly involves a light chain-binding region.

1987 ◽  
Vol 105 (5) ◽  
pp. 2011-2019 ◽  
Author(s):  
G S Blank ◽  
F M Brodsky
Keyword(s):  
Monoclonal Antibodies ◽  
Binding Site ◽  
Heavy Chain ◽  
Light Chain ◽  
Intermediate Filament ◽  
Binding Sites ◽  
Light Chains ◽  
Binding Region ◽  
Intermediate Filament Proteins ◽  
Interaction Site

Two regions on the clathrin heavy chain that are involved in triskelion interactions during assembly have been localized on the triskelion structure. These regions were previously identified with anti-heavy chain monoclonal antibodies X19 and X35, which disrupt clathrin assembly (Blank, G. S., and F. M. Brodsky, 1986, EMBO (Eur. Mol. Biol. Organ.) J., 5:2087-2095). Antibody-binding sites were determined based on their reactivity with truncated triskelions, and were mapped to an 8-kD region in the middle of the proximal portion of the triskelion arm (X19) and a 6-kD region at the triskelion elbow (X35). The elbow site implicated in triskelion assembly was also shown to be included within a heavy chain region involved in binding the light chains and to constitute part of the light chain-binding site. We postulate that this region of the heavy chain binds to the interaction site identified on the light chains that has homology to intermediate filament proteins (Brodsky, F. M., C. J. Galloway, G. S. Blank, A. P. Jackson, H.-F. Seow, K. Drickamer, and P. Parham, 1987, Nature (Lond.), 326:203-205). These findings suggest the existence of a heavy chain site, near the triskelion elbow, which is involved in both intramolecular and intermolecular interactions during clathrin assembly.

Differentiation of the Major Human Tumor Groups using Conventional and Monoclonal Antibodies Specific for Individual Intermediate Filament Proteins

1985 ◽  
Vol 455 (1 Intermediate) ◽  
pp. 649-668 ◽  
Author(s):  
MARY OSBORN ◽  
MICHAEL ALTMANNSBERGER ◽  
ELKE DEBUS ◽  
KLAUS WEBER
Keyword(s):  
Monoclonal Antibodies ◽  
Intermediate Filament ◽  
Human Tumor ◽  
Intermediate Filament Proteins
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