scholarly journals Identification and characterization of polyubiquitin gene from cDNA library of aspergillus fumigatus

2005 ◽  
Vol 20 (1) ◽  
pp. 208-212 ◽  
Author(s):  
Jata Shankar ◽  
Taruna Madan ◽  
Seemi Farhat Basir ◽  
P. Usha Sarma
2016 ◽  
Vol 36 (7) ◽  
pp. 591-594 ◽  
Author(s):  
Andréia Spanamberg ◽  
Laerte Ferreiro ◽  
Gustavo Machado ◽  
Cibele Floriano Fraga ◽  
Ricardo Araujo

Abstract: Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung's samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung's samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of β-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung's samples of broilers.


HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 480d-480
Author(s):  
David G. Clark ◽  
Kathleen B. Evensen

Ethylene-regulated gene expression is being studied in several plant systems, but the exact mechanism of ethylene action during plant development and senescence is poorly understood. When geranium (Pelargonium Xhortorum) flowers are exposed to 1 μ1/L of ethylene gas for 1 hour, petals begin to abscise within 60-90 minutes from the start of treatment, The rapidity of the response implies that it must be very direct. We now demonstrate that ethylene acts at the level of message accumulation. We have constructed a cDNA library from mRNA isolated from ethylene-treated geranium gynoecia. Ethylene-induced clones have been isolated by differential screening of this library with cDNA probes synthesized from ethylene-treated and untreated geranium gynoecia mRNA. Identification and characterization of these clones will be discussed.


2021 ◽  
Vol 131 (1) ◽  
pp. 1-7
Author(s):  
Emiko Matsunaga ◽  
Yutaka Tanaka ◽  
Saki Toyota ◽  
Hisae Yamada ◽  
Takuji Oka ◽  
...  

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48845 ◽  
Author(s):  
Fabrizio Cavazzini ◽  
Riccardo Magistroni ◽  
Luciana Furci ◽  
Valentina Lupo ◽  
Giulia Ligabue ◽  
...  

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