Role of the leukemia-inhibitory factor gene mutations in infertile women: The embryo-endometrial cytokine cross talk during implantation — a delicate homeostatic equilibrium

2005 ◽  
Vol 50 (3) ◽  
pp. 179-186 ◽  
Author(s):  
M. Králíčková ◽  
P. Šíma ◽  
Z. Rokyta
Keyword(s):  
Cross Talk ◽  
Leukemia Inhibitory Factor ◽  
Gene Mutations ◽  
Inhibitory Factor ◽  
Factor Gene ◽  
Infertile Women

On the search for the genetic background of the primary idiopathic infertility: the leukemia-inhibitory factor gene mutations in the population of infertile women

2003 ◽  
Vol 80 ◽  
pp. 89-90 ◽  
Author(s):  
Milena Beranova ◽  
Petr Sima ◽  
Zdenek Rokyta ◽  
Zdenka Ulcova-Gallova ◽  
Tomas Vanecek ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Genetic Background ◽  
Gene Mutations ◽  
Inhibitory Factor ◽  
Factor Gene ◽  
Infertile Women ◽  
Idiopathic Infertility

Leukemia inhibitory factor gene mutations in the population of infertile women are not restricted to nulligravid patients

2006 ◽  
Vol 127 (2) ◽  
pp. 231-235 ◽  
Author(s):  
Milena Kralickova ◽  
Radek Sima ◽  
Tomas Vanecek ◽  
Petr Sima ◽  
Zdenek Rokyta ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Gene Mutations ◽  
Inhibitory Factor ◽  
Factor Gene ◽  
Infertile Women

Association of the leukemia inhibitory factor gene mutation and the antiphospholipid antibodies in the peripheral blood of infertile women

2007 ◽  
Vol 52 (5) ◽  
pp. 543-548 ◽  
Author(s):  
M. Králíčková ◽  
Z. Ulčová-Gallová ◽  
R. Šíma ◽  
T. Vaněček ◽  
P. Šíma ◽  
...  
Keyword(s):  
Gene Mutation ◽  
Peripheral Blood ◽  
Leukemia Inhibitory Factor ◽  
Antiphospholipid Antibodies ◽  
Inhibitory Factor ◽  
Factor Gene ◽  
Infertile Women

The role of the leukemia inhibitory factor receptor in embryo implantation and placentation

Placenta ◽  
2021 ◽  
Vol 114 ◽  
pp. 139
Author(s):  
Jumpei Terakawa ◽  
Kazuhiro Matsuo ◽  
Takafumi Namiki ◽  
Kana Ohtomo ◽  
Atsuko Kageyama ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Embryo Implantation ◽  
Inhibitory Factor ◽  
Leukemia Inhibitory Factor Receptor

A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor

2002 ◽  
Vol 269 (11) ◽  
pp. 2716-2726 ◽  
Author(s):  
Andreas Timmermann ◽  
Andrea Küster ◽  
Ingo Kurth ◽  
Peter C. Heinrich ◽  
Gerhard Müller-Newen
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Functional Role ◽  
Inhibitory Factor ◽  
Signal Transducer ◽  
Leukemia Inhibitory Factor Receptor ◽  
Extracellular Domains

272 LEUKEMIA INHIBITORY FACTOR SIGNALING PATHWAY IN PIG PARTHENOGENETIC PLURIPOTENT CELLS

2009 ◽  
Vol 21 (1) ◽  
pp. 233
Author(s):  
T. A. L. Brevini ◽  
G. Pennarossa ◽  
S. Antonini ◽  
F. Gandolfi
Keyword(s):  
Molecular Markers ◽  
Signaling Pathway ◽  
Leukemia Inhibitory Factor ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Inhibitory Factor ◽  
Germ Layers ◽  
Undifferentiated State ◽  
Undifferentiated Cells

Leukemia inhibitory factor (LIF), its receptor heterodimer (LRβ-gp130), and the related signal transducer and activator of transcription-3 (STAT3) constitute a system controlling self-renewal and pluripotency of embryonic stem cells (ESC) in the mouse, where LIF withdrawal or direct inhibition of STAT3 causes ESC differentiation. By contrast, several studies have demonstrated that LIF is not required to maintain human ESC pluripotency. Scattered information is available in other species, and data on the role of LIF in pig ESC are scanty. The aims of the present study were (a) to characterize the expression profile of gp130, LRβ, and STAT3 in pig parthenogenetic cell lines (ppC), previously derived in our laboratory and shown to be positive for the main pluripotency related markers; (b) to evaluate the role of LIF pathway in maintaining the pluripotency of these cells. To this purpose, ppC were cultured as previously described (Brevini et al. 2007 Theriogenology 68, 206–214) and screened by RT-PCR for the two LIF receptor subunits (LRβ and gp130) and STAT3. Pig granulosa cells were used as positive controls. To better investigate the possible role of LIF in maintenance of pluripotency in ppC, the formation of embryoid bodies (EB) was induced in the presence or in the absence of the cytokine. Undifferentiated cells were cultured in hanging drops either with or without LIF for 12 days. The EB formation and the expression of molecular markers specific for the three germ layers was evaluated at the end of the differentiation period. Molecular analysis allowed us to detect transcription of STAT3, whereas no signal for LRβ and gp130 was detected in ppC. These results seem to indicate that LIF does not play a role in the maintenance of pluripotency in the pig. However, after removal of LIF, ppC routinely formed EB that expressed molecular markers specific for the three germ layers. On the other hand, when LIF was added to the differentiation medium, pig cells were unable to form EB. They kept proliferating in an undifferentiated state, and no expression of molecular markers specific for the three germ layers was detected. Moreover, when re-plated on inactivated feeder-layers, they formed distinct colonies that maintained expression of pluripotency markers. Our results show that a role of LIF in pluripotency maintenance through a classical LRβ-gp130 and STAT3 activation pathway is unlikely. However, interaction with an alternative nonclassical activation signaling pathway cannot be ruled out. Indeed, the presence of the cytokine in the medium used for differentiation experiments actively inhibited EB formation, indicating a possible role in preventing differentiation in the porcine species. Further studies are needed to elucidate these aspects. Supported by: PRIN2005; PRIN2006; First 2006; First2007.

scholarly journals LEUKEMIA INHIBITORY FACTOR IN RECURRENT PREGNANCY LOSS: IS THERE ANY ROLE OF LIFESTYLE INTERVENTION?

2020 ◽  
Vol 114 (3) ◽  
pp. e195
Author(s):  
Vidhu Dhawan ◽  
Neena Malhotra ◽  
Neeta Singh ◽  
Vatsla Dadhwal ◽  
Rima Dada
Keyword(s):  
Lifestyle Intervention ◽  
Leukemia Inhibitory Factor ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Inhibitory Factor
Sign in / Sign up

Export Citation Format

Share Document