Minimizing phenol pollution in sugarcane stem apical meristem culture

Sugar Tech ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 297-300 ◽  
Author(s):  
Cheng-Mei Huang ◽  
Yang-Rui Li ◽  
Yan-Ping Ye
Keyword(s):  
Apical Meristem ◽  
Meristem Culture ◽  
Phenol Pollution ◽  
Sugarcane Stem ◽  
Stem Apical Meristem

Elimination of sugarcane grassy shoot disease through apical meristem culture

2011 ◽  
Vol 44 (20) ◽  
pp. 1942-1948 ◽  
Author(s):  
A. K. Tiwari ◽  
S. Tripathi ◽  
M. Lal ◽  
M. L. Sharma ◽  
P. Chiemsombat
Keyword(s):  
Apical Meristem ◽  
Meristem Culture ◽  
Sugarcane Grassy Shoot Disease ◽  
Grassy Shoot Disease

scholarly journals Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture

2019 ◽  
Vol 46 (3) ◽  
pp. 228-235
Author(s):  
Young Hee Kwon ◽  
Joung Kwan Lee ◽  
Hee Kyu Kim ◽  
Kyung Ok Kim ◽  
Jae Seong Park ◽  
...  
Keyword(s):  
Apical Meristem ◽  
Meristem Culture ◽  
Virus Elimination

scholarly journals Sanitization of potato varieties from the VIR collection against viruses

2021 ◽  
Vol 181 (4) ◽  
pp. 164-172
Author(s):  
E. S. Bespalova ◽  
M. M. Agakhanov ◽  
S. B. Arkhimandritova ◽  
M. V. Erastenkova ◽  
Yu. V. Uhatova
Keyword(s):  
Solanum Tuberosum ◽  
Apical Meristem ◽  
Multiple Infections ◽  
Meristem Culture ◽  
Close Monitoring ◽  
High Quality ◽  
Potato Plants ◽  
Planting Material ◽  
Potato Collection

Background. VIR’s potato collection is one of the oldest and richest; however, it is constantly exposed to viruses that negatively affect useful agronomic properties of tubers. Close monitoring of the phytosanitary state of potato accessions helps to select the most effective method of therapy for subsequent healing of infected plants and obtaining high-quality planting material.Materials and methods. The research was aimed at improving the health of 18 varieties of Solanum tuberosum L. from the VIR collection. Testing for the presence of viruses was based on the ICA and RTPCR techniques, and the consequent healing was performed using the methods of meristem culture and cryotherapy.Results and conclusions. During the field test of potato plants, PVX, PVS and PVA were found to be the most common viruses. PSTVd was completely absent in all tested accessions. The effectiveness of in vitro healing of potato plants from viruses was assesses using meristem culture. The percentage of healed plants was 0% for PVS, 0% for PVX, 33.4% for PVA, 50% for PLRV, 72.3% for PVY, and 83.4% for PVM. Healing with meristem culture was shown to be the most effective against PVY and PVM. While assessing the effectiveness of post-cryogenic restoration of potato microplants, the level of post-cryogenic regeneration of the shoot tips in potato microplants was determined at 22.3% on average for a sample. The minimum was observed in k-16762 ‘Sagita N’ (5%), and the maximum in k-1378 ‘Marta’ (41.7%). Analysis of the effectiveness of potato recovery from viruses by in vitro cryotherapy showed that the percentage of recovered plants was 100% for PVY, 100% for PVA, 88.9% for PVM, 77.8% for PVS, 44.4% for PVX. Thus, the techniques of apical meristem culture and cryotherapy proved to be effective against PVY, PVA and PVM viruses. However, in the case of multiple infections, it is necessary to combine elements of different healing protocols to increase the effectiveness of the healing procedure. 

scholarly journals The regeneration potential of promising winter common wheat lines in shoot apical meristem culture

2019 ◽  
Vol 25 ◽  
pp. 298-303
Author(s):  
S. V. Pykalo ◽  
O. A. Demydov ◽  
T. V. Yurchenko ◽  
N. I. Prokopik ◽  
O. V. Humeniuk
Keyword(s):  
Common Wheat ◽  
Shoot Regeneration ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Plant Production ◽  
Meristem Culture ◽  
Culture Methods ◽  
Regeneration Potential ◽  
Regenerated Plants ◽  
Wheat Lines

Aim. To investigate the regenerative ability of promising winter common wheat lines in shoot apical meristem culture. Methods. Plant tissue culture methods, statistical evaluation of data. Results. The processes of morphogenesis in culture of apical meristem of 3-days seedlings of lines of winter common wheat were investigated and it was established that the frequency of callusogenesis and shoot regeneration in the studied forms is determined by the genotype of explant. Two types of callus with morphophysiological properties were identified: morphogenic and nonmorphogenic callus. The formation of regenerated plants from wheat calli took place through both gemmorizogenesis and somatic embryogenesis. Conclusions. The line Erytrospermum 60068 was characterized the highest regeneration potential and it can be recommended for further biotechnology of wheat. Obtained technology of vigorous regenerated plant production of winter common wheat lines in shoot apical meristem culture can be used in cell selection and genetic engineering experiments. Keywords: winter common wheat, apical meristem, genotype, callus, shoot regeneration.

scholarly journals In vitro regeneration of pumpkin (cucurbita maxima) through shoot apical meristem

1970 ◽  
Vol 18 ◽  
pp. 104-107 ◽  
Author(s):  
ME Haque ◽  
D Rezwana ◽  
MA Islam ◽  
B Sikdar
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Total Yield ◽  
Meristem Culture ◽  
Culture Vessel ◽  
Cucurbita Maxima ◽  
Indirect Regeneration

Context: Pumpkin (Cucurbita maxima) is very important vegetable crop. It is damaged by several types of diseases, especially viral diseases which reduce the total yield of pumpkin. This is very difficult problem and such type of problem can be overcome by meristem culture. Objectives: To develop suitable protocols for indirect regeneration of pumpkin plant through shoot apical meristem.   Materials and Methods: Meristems were isolated from 15-21 days in vivo grown plants by collecting shoot tips and were prepared (0.2-0.5 mm) under 4X zoom stereo-microscope remaining two leaves primordia. Inoculation of explants was made singly per culture vessel in semisolid MS fortified with different concentrations and combinations of cytokinin and auxin.   Results: Among different concentrations, 1.0 mg/l BAP showed best response for callus induction. Calli were sub cultured for shoot formation in MS containing BAP singly or combination with GA3 and 1.5 mg/l BAP + 0.1 mg/l GA3 gave better result. In vitro regenerated shoots were rooted well in ½ strength MS with 0.5 mg/l IBA and micro plants were acclimatized successfully in natural condition.   Conclusion: Indirect regeneration of pumpkin plants through shoot apical meristem has been established.   Keywords: Growth regulators; in vitro regeneration; Pumpkin; Cucurbita maxima. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8784 JBS 2010; 18(0): 104-107

scholarly journals Production of Tomato Yellow Leaf Curl Virus-free Parthenocarpic Tomato Plants by Leaf Primordia-free Shoot Apical Meristem Culture Combined with in vitro Grafting

2015 ◽  
Vol 84 (4) ◽  
pp. 327-333 ◽  
Author(s):  
Sota Koeda ◽  
Rihito Takisawa ◽  
Tomoyuki Nabeshima ◽  
Yuri Tanaka ◽  
Akira Kitajima
Keyword(s):  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Tomato Yellow Leaf ◽  
Leaf Primordia ◽  
Meristem Culture ◽  
Yellow Leaf ◽  
Tomato Plants ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals A Member of the Closteroviridae from Mint with Similarities to All Three Genera of the Family

Plant Disease ◽  
2005 ◽  
Vol 89 (6) ◽  
pp. 654-658 ◽  
Author(s):  
Ioannis E. Tzanetakis ◽  
Joseph D. Postman ◽  
Robert R. Martin
Keyword(s):  
Apical Meristem ◽  
Meristem Culture ◽  
Double Stranded Rna ◽  
Important Link ◽  
The Family ◽  
Heat Therapy ◽  
Sequence Similarities

Mentha × gracilis ‘Variegata’, described more than 200 years ago, is still being used as an ornamental. The bright vein-banding symptoms that confer the ornamental value to ‘Variegata’ clones are graft transmissible and can be eliminated after heat therapy and apical meristem culture. This observation led us to investigate the possibility that symptoms are virus-induced. Double-stranded RNA extracted from a ‘Variegata’ clone was cloned. One of the viruses identified was a member of the Closteroviridae family. This virus, designated Mint vein-banding associated virus, shares sequence similarities with all three genera of the family, making it an important link among the genera of the Closteroviridae. A detection protocol has been developed that readily detects the virus in other mint clones that exhibit vein-banding symptoms.

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