Verticillium dahliae interactions with V. albo-atrum and V. tricorpus and their effects on verticillium wilt disease development in potato

2007 ◽  
Vol 84 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Natasha Robinson ◽  
H. W. Piatt ◽  
Lawrence R. Hale
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Wilt Disease ◽  
Disease Development

scholarly journals Verticillium Wilt Disease of Various Crops Caused by Verticillium dahliae Klebahn

1980 ◽  
Vol 46 (2) ◽  
pp. 267-270
Author(s):  
Kenji KITAZAWA ◽  
Takahito SUZUI
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Wilt Disease

Effect of nitrogen on the incidence of Verticillium wilt disease of egg plant caused by Verticillium dahliae Kleb

1974 ◽  
Vol 40 (1) ◽  
pp. 217-220 ◽  
Author(s):  
K. Sivaprakasam ◽  
C. K. Soumini Rajagopalan
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Wilt Disease ◽  
Egg Plant ◽  
Verticillium Dahliae Kleb

scholarly journals Verticillium Wilt of African Daisy (Osteospermum sp.) in Italy Caused by Verticillium dahliae

Plant Disease ◽  
2005 ◽  
Vol 89 (6) ◽  
pp. 688-688
Author(s):  
A. Garibaldi ◽  
A. Minuto ◽  
M. L. Gullino
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Plant Disease ◽  
Vascular Tissue ◽  
Northern Italy ◽  
Wilt Disease ◽  
Pathogenicity Test ◽  
Conidial Suspension ◽  
Average Temperature ◽  
Food Agric

During the winter of 2004, container-grown African daisy (Osteospermum sp.) plants, cv Seaside, showing symptoms of a wilt disease were observed in an open field in Albenga located in northern Italy. Symptoms were first observed on 120-day-old plants grown in a peat/composted bark/clay/pumice (50:20:10:20) substrate. The vascular tissues of affected plants appeared brown. These plants were stunted and developed yellow leaves with brown or black streaks in the vascular tissue. Verticillium dahliae was consistently and readily isolated from symptomatic vascular tissue when cultured on potato dextrose agar. Healthy rooted plants (40-day-old cv Seaside) were inoculated by root dip with a conidial suspension (5 × 107 CFU/ml) from one of three isolates of V. dahliae isolated from infected plants and transplanted into pots filled with steam-sterilized soil. Noninoculated plants served as control treatments. Plants (10 per treatment) were grown in a glasshouse at an average temperature of 25°C. First wilt symptoms and vascular discoloration in the roots, crown, and veins developed within 15 days on each inoculated plant and become very evident after 50 days. Noninoculated plants remained healthy. V. dahliae was consistently reisolated from inoculated plants. The pathogenicity test was conducted twice. To our knowledge, this is the first report of V. dahliae on Osteospermum sp. in Italy and in Europe. Verticillium wilt has been previously reported on O. fruticosum in California (1). Reference: (1) A. M. French. California Plant Disease Host Index. Calif. Dep. Food Agric. 1989.

scholarly journals Occurrence of Wilt Disease Caused by Verticillium dahliae on Ailanthus glandulosa in Greece

Plant Disease ◽  
1998 ◽  
Vol 82 (1) ◽  
pp. 129-129 ◽  
Author(s):  
G. Skarmoutsos ◽  
H. Skarmoutsou
Keyword(s):  
Verticillium Wilt ◽  
Cross Sections ◽  
Verticillium Dahliae ◽  
Tree Species ◽  
Wilt Disease ◽  
Vascular Wilt ◽  
Northern Greece ◽  
Globular Cells ◽  
Short Time ◽  
Different Parts

Ailanthus glandulosa is an introduced tree species in Greece used as ornamental and is considered resistant to both fungal and insect attacks. However, since 1992, a great number of A. glandulosa plants of different ages and in different parts of northern Greece have been dying. The phenomenon appeared either in single trees or in groups, and the number of affected plants increased from year to year. The symptoms were characteristic of a wilt disease. Dieback started on one or more branches and gradually spread, or whole trees were affected from the beginning. Death may occur during spring when trees do not resume their activity or later in the summer. Leaves wilt, turn yellow and fall in a short time. Trees may die in 1 to 3 years depending on their age. In cross sections of branches a continuous discoloured ring or arc was present in the outermost sapwood. Isolations made from dying trees from 25 different locations have yielded V. dahliae, a pathogen known to cause vascular wilt on many plant hosts, including Ailanthus (1). Isolations made from healthy trees did not yield the fungus. Abundant mycelium developed in the host vessels of infected trees; the hyphae were cylindrical, hyaline, and septate. On malt agar fungal cultures developed quickly and were initially a whitish to cream color that later turned black due to the formation of microsclerotia. In culture, microsclerotia started to form centrally and were dark brown to black, consisting of swollen globular cells that formed irregular masses variable in shape. The fungus also produced abundant verticillate conidiophores with phialides and conidia. This is a first report of Verticillium wilt on A. glandulosa in Greece. Reference: (1) G. Arnaud et al. Rev. Pathol. Veg. Entomol. Agric. Fr. 17:227, 1930.

scholarly journals Verticillium Wilt of Xanthium italicum Caused by Verticillium dahliae in Italy

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 908-908
Author(s):  
I. Camele ◽  
C. Marcone ◽  
A. Caponero ◽  
A. Ambrico ◽  
M. Mucci ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Cross Sections ◽  
Verticillium Dahliae ◽  
Pure Culture ◽  
Late Summer ◽  
Wilt Disease ◽  
Nuclear Ribosomal Dna ◽  
Conidial Suspension ◽  
Italian Isolate ◽  
Total Dna

During the late summer of 2003, a wilt disease of the weed Italian cockleburr (Xanthium italicum Mor.) was observed in the Basilicata Region of southern Italy. Diseased plants were growing near an apricot orchard in which some trees were severely affected by Verticillium wilt. The most characteristic symptoms of the wilt disease affecting Italian cockleburr were yellowing, stunting, and gradual wilting. Also, diagonal and cross sections of stems revealed brown discoloration of their vascular tissues. To elucidate the etiology of the disease, we attempted detection and identification of the causal agents using traditional and polymerase chain reaction (PCR)-based methods. Small pieces of petiole and stem tissues from diseased and asymptomatic plants were surface disinfested in NaOCl solution, rinsed in sterile distilled water, blotted dry, and plated onto water agar (WA) medium. Following incubation at 22°C, the emerging colonies were transferred to potato dextrose agar (PDA). Verticillium dahliae (one isolate) was consistently identified on the basis of its morphological features according to the description of Smith (2). Using PCR assays with the primer pair ITS5/ITS4 (3), which are directed to fungal nuclear ribosomal DNA (rDNA) repeat sequences, an amplification product of approximately 560 bp was obtained by using total DNA extracted from wilt-affected Italian cockleburr plant tissues (five plants examined) as well as fresh mycelium from the V. dahliae-infected Italian cockleburr pure culture-maintained isolate mentioned above. No visible PCR products were obtained with total DNA from asymptomatic Italian cockleburr plants. Sequence analysis of the ITS5/ITS4 amplicons revealed no differences in their nucleotide positions. The obtained sequence of the V. dahliae-infected Italian cockleburr isolate (GenBank Accession No. AJ865691) was then used as query sequences in a BLAST 2.0 search (1). Sequence of the southern Italian isolate proved to be identical to that of the Greek strain “76 Greece” of V. dahliae (GenBank Accession no. AF104926). To prove Koch's postulates, 10 healthy Italian cockleburr seedlings were experimentally inoculated by dipping trimmed roots in a single-conidial suspension (1.5 × 106 CFU/ml) obtained from 10-day-old colonies of the V. dahliae-infected Italian cockleburr pure culture-maintained isolate. After 4 weeks, all inoculated Italian cockleburr plants showed symptoms identical to those of naturally infected field-grown plants. V. dahliae was consistently reisolated from inoculated plants. Additional inoculation experiments revealed that pepper and eggplant were also susceptible to the V. dahliae-infected Italian cockleburr isolate showing typical Verticillium wilt symptoms. To our knowledge, this is the first report of the occurrence of Verticillium wilt of X. italicum. References: (1) S. F. Altschul et al. Nucleic Acids Res. 25:3389, 1997. (2) H. C. Smith. N.Z. J. Agric. Res. 8:450, 1965. (3) T. J. White et al. Pages 315–322 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.

Interactions of variousverticillium species in combination withV. albo-atrum on verticillium wilt disease development in potato

2007 ◽  
Vol 84 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Natasha Robinson ◽  
H. W. (Bud) Platt ◽  
Lawrence R. Hale
Keyword(s):  
Verticillium Wilt ◽  
Wilt Disease ◽  
Disease Development

scholarly journals GhKWL1 Upregulates GhERF105 but Its Function Is Impaired by Binding with VdISC1, a Pathogenic Effector of Verticillium dahliae

2021 ◽  
Vol 22 (14) ◽  
pp. 7328
Author(s):  
Yang Chen ◽  
Mi Zhang ◽  
Lei Wang ◽  
Xiaohan Yu ◽  
Xianbi Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Gossypium Hirsutum ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Upland Cotton ◽  
Effector Protein ◽  
Positive Regulator ◽  
Its Gene

Verticillium wilt, caused by Verticillium dahliae, is a devastating disease for many important crops, including cotton. Kiwellins (KWLs), a group of cysteine-rich proteins synthesized in many plants, have been shown to be involved in response to various phytopathogens. To evaluate genes for their function in resistance to Verticillium wilt, we investigated KWL homologs in cotton. Thirty-five KWL genes (GhKWLs) were identified from the genome of upland cotton (Gossypium hirsutum). Among them, GhKWL1 was shown to be localized in nucleus and cytosol, and its gene expression is induced by the infection of V. dahliae. We revealed that GhKWL1 was a positive regulator of GhERF105. Silencing of GhKWL1 resulted in a decrease, whereas overexpression led to an increase in resistance of transgenic plants to Verticillium wilt. Interestingly, through binding to GhKWL1, the pathogenic effector protein VdISC1 produced by V. dahliae could impair the defense response mediated by GhKWL1. Therefore, our study suggests there is a GhKWL1-mediated defense response in cotton, which can be hijacked by V. dahliae through the interaction of VdISC1 with GhKWL1.

RESISTANCE TO VERTICILLIUM WILT IN F1 GENERATIONS OF SELF-FERTILIZED SPECIES OF FRAGARIA

1958 ◽  
Vol 36 (3) ◽  
pp. 297-299 ◽  
Author(s):  
W. Newton ◽  
M. C. J. van Adrichem
Keyword(s):  
Verticillium Wilt ◽  
Wilt Disease ◽  
Fragaria Chiloensis

The F1 generation of selfed plants of Fragaria chiloensis, F. ovalis, and F. yukonensis contained seedlings resistant to the verticillium wilt disease. Selfed F. orientalis plants yielded seedlings that carried considerable tolerance but selfed F. vesca, F. bracteata, and F. virginiana plants yielded neither tolerant nor resistant seedlings. Asexually propagated plants of the seven species were all susceptible to the disease.

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