scholarly journals In situ hybridization histochemistry of Spot 35 protein, a calcium-binding protein, in the rat brain

1991 ◽  
Vol 15 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Hiroshi Usui ◽  
Takashi Katagiri ◽  
Yasuji Yoshida ◽  
Akiko Nishiyama ◽  
Tomio Ichikawa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Calcium Binding ◽  
Binding Protein ◽  
Protein A ◽  
Calcium Binding Protein ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry

scholarly journals In situ detection of vitamin D-induced calcium-binding protein (9-kDa CaBP) messenger RNA in rat duodenum.

1986 ◽  
Vol 34 (2) ◽  
pp. 277-280 ◽  
Author(s):  
M Warembourg ◽  
O Tranchant ◽  
C Perret ◽  
C Desplan ◽  
M Thomasset
Keyword(s):  
Vitamin D ◽  
In Situ Hybridization ◽  
Calcium Binding ◽  
Binding Protein ◽  
Cdna Probe ◽  
Calcium Binding Protein ◽  
Pbr322 Dna ◽  
Rat Duodenum ◽  
Columnar Cells

We have previously described the molecular cloning of a cDNA fragment synthesized from rat duodenal mRNA coding for a 9000-dalton vitamin D-induced calcium-binding protein (9-kDa CaBP) (3). We now report the use of this cloned cDNA to study the cytological distribution of 9-kDa CaBP mRNA in rat duodenum by in situ hybridization. Tissue sections, fixed in ethanol:acetic acid, were hybridized to the 3H-cDNA probe and processed for autoradiography. The specificity of the CaBP mRNA-DNA hybrid formation was checked using 3H-labeled plasmid pBR322 DNA as a control probe. 9k-Da CaBP mRNA, visualized by silver grains, was found only in the absorptive epithelial cells, and the concentration was greater in the cells at the villous tips than in those of the crypts. The 9k-Da CaBP mRNA was observed mainly in the cytoplasm of the columnar cells and less frequently in the nucleus. Labeling was not seen in the brush border and goblet cells. The submucosa, with Brunner's glands and muscularis, also showed no specific 9-kDa CaBP mRNA concentration. This demonstration of 9-kDa CaBP gene activity in the columnar cells of the rat duodenum illustrates the usefulness of in situ hybridization for characterization of specific cells involved in the expression of 1,25(OH)2 D3 activity.

Expression of the mRNAs for the Kv3.1 potassium channel gene in the adult and developing rat brain

1992 ◽  
Vol 68 (3) ◽  
pp. 756-766 ◽  
Author(s):  
T. M. Perney ◽  
J. Marshall ◽  
K. A. Martin ◽  
S. Hockfield ◽  
L. K. Kaczmarek
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
K Channel ◽  
Rnase Protection ◽  
Hybridization Histochemistry ◽  
Adult Rat ◽  
In Situ Hybridization Histochemistry ◽  
Adult Rat Brain ◽  
Developing Rat

1. The gene for a mammalian Shaw K+ channel has recently been cloned and has been shown, by alternative splicing, to give rise to two different transcripts, Kv3.1 alpha and Kv3.1 beta. To determine whether these channels are associated with specific types of neurons and to determine whether or not the alternately spliced K+ channel variants are differentially expressed, we used ribonuclease (RNase) protection assays and in situ hybridization histochemistry to localize the specific subsets of neurons containing Kv3.1 alpha and Kv3.1 beta mRNAs in the adult and developing rat brain. 2. In situ hybridization histochemistry revealed a heterogeneous expression pattern of Kv3.1 alpha mRNA in the adult rat brain. Highest Kv3.1 alpha mRNA levels were expressed in the cerebellum. High levels of hybridization were also detected in the globus pallidus, subthalamus, and substantia nigra reticulata. Many thalamic nuclei, but in particular the reticular thalamic nucleus, hybridized well to Kv3.1 alpha-specific probes. A subpopulation of cells in the cortex and hippocampus, which by their distribution and number may represent interneurons, were also found to contain high levels of Kv3.1 alpha mRNA. In the brain stem, many nuclei, including the inferior colliculus and the cochlear and vestibular nuclei, also express Kv3.1 alpha mRNA. Low or undetectable levels of Kv3.1 alpha mRNA were found in the caudate-putamen, olfactory tubercle, amygdala, and hypothalamus. 3. Kv3.1 beta mRNA was also detected in the adult rat brain by both RNase protection assays and by in situ hybridization experiments. Although the beta splice variant is expressed at lower levels than the alpha species, the overall expression pattern for both mRNAs is similar, indicating that both splice variants co-expressed in the same neurons. 4. The expression of Kv3.1 alpha and Kv3.1 beta transcripts was examined throughout development. Kv3.1 alpha mRNA is detected as early as embryonic day 17 and then increases gradually until approximately postnatal day 10, when there is a large increase in the amount of Kv3.1 alpha mRNA. Interestingly, the expression of Kv3.1 beta mRNA only increases gradually during the developmental time frame examined. Densitometric measurements indicated that Kv3.1 alpha is the predominant splice variant found in neurons of the adult brain, whereas Kv3.1 beta appears to be the predominant species in embryonic and perinatal neurons. 5. Most of the neurons that express the Kv3.1 transcripts have been characterized electrophysiologically to have narrow action potentials and display high-frequency firing rates with little or no spike adaptation.(ABSTRACT TRUNCATED AT 400 WORDS)

Localization of glycine receptor α1 subunit mRNA-containing neurons in the rat brain: An analysis using in situ hybridization histochemistry

Neuroscience ◽  
1991 ◽  
Vol 43 (2-3) ◽  
pp. 381-395 ◽  
Author(s):  
K. Sato ◽  
J.-H. Zhang ◽  
T. Saika ◽  
M. Sato ◽  
K. Tada ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Glycine Receptor ◽  
Hybridization Histochemistry ◽  
Subunit Mrna ◽  
In Situ Hybridization Histochemistry ◽  
Α1 Subunit

In situ hybridization histochemistry: localisation of vasopressin mRNA in rat brain using a biotinylated oligonucleotide probe

1988 ◽  
Vol 4 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Heii Arai ◽  
Piers C. Emson ◽  
Sudhir Agrawal ◽  
Chris Christodoulou ◽  
Michael J. Gait
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Oligonucleotide Probe ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry

Localization of mRNAs for calpain and calpastatin in the adult rat brain by in situ hybridization histochemistry

1994 ◽  
Vol 23 (1-2) ◽  
pp. 40-46 ◽  
Author(s):  
Kaoru Goto ◽  
Tatsuaki Iwamoto ◽  
Hisatake Kondo
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Hybridization Histochemistry ◽  
Adult Rat ◽  
In Situ Hybridization Histochemistry ◽  
Adult Rat Brain

Localization of the gap junction mRNA in the neonatal rat brain by in situ hybridization histochemistry

1991 ◽  
Vol 14 ◽  
pp. S100
Author(s):  
Akira Matsumoto ◽  
Yasumasa Arai ◽  
Akihisa Urano ◽  
Susumu Hyodo
Keyword(s):  
In Situ Hybridization ◽  
Gap Junction ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Neonatal Rat Brain

Distribution of the beta-2 adrenergic receptor messenger RNA in the rat brain by in situ hybridization histochemistry: Effects of chronic reserpine treatment

1991 ◽  
Vol 16 (12) ◽  
pp. 1253-1256 ◽  
Author(s):  
M. Asanuma ◽  
N. Ogawa ◽  
K. Mizukawa ◽  
K. Hara ◽  
H. Hirata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Adrenergic Receptor ◽  
Messenger Rna ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
Reserpine Treatment ◽  
Beta 2
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