Nucleic acid spin labeling study of interaction between polyadenylic acid: Polyuridilic acid duplex and egg phosphatidylcholine vesicles. Involvement of terminal and internal nucleotides and sugar-phosphate backbone

1994 ◽  
Vol 7 (1) ◽  
pp. 115-130 ◽  
Author(s):  
R. I. Zhdanov ◽  
L. A. Volkova ◽  
V. V. Kuvichkin ◽  
A. I. Petrov
Keyword(s):  
Nucleic Acid ◽  
Spin Labeling ◽  
Sugar Phosphate ◽  
Phosphate Backbone ◽  
Polyadenylic Acid

scholarly journals Convertible and Constrained Nucleotides: The 2’-Deoxyribose 5’-C-Functionalization Approach, a French Touch

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5925
Author(s):  
Crystalle Chardet ◽  
Corinne Payrastre ◽  
Béatrice Gerland ◽  
Jean-Marc Escudier
Keyword(s):  
Nucleic Acid ◽  
Conformational Space ◽  
Biological Applications ◽  
Sugar Phosphate ◽  
Conformationally Constrained ◽  
Precise Control ◽  
Phosphate Backbone ◽  
Nucleophilic Displacement ◽  
Torsional Angles ◽  
Biotechnical Properties

Many strategies have been developed to modulate the biological or biotechnical properties of oligonucleotides by introducing new chemical functionalities or by enhancing their affinity and specificity while restricting their conformational space. Among them, we review our approach consisting of modifications of the 5’-C-position of the nucleoside sugar. This allows the introduction of an additional chemical handle at any position on the nucleotide chain without disturbing the Watson–Crick base-pairing. We show that 5’-C bromo or propargyl convertible nucleotides (CvN) are accessible in pure diastereoisomeric form, either for nucleophilic displacement or for CuAAC conjugation. Alternatively, the 5’-carbon can be connected in a stereo-controlled manner to the phosphate moiety of the nucleotide chain to generate conformationally constrained nucleotides (CNA). These allow the precise control of the sugar/phosphate backbone torsional angles. The consequent modulation of the nucleic acid shape induces outstanding stabilization properties of duplex or hairpin structures in accordance with the preorganization concept. Some biological applications of these distorted oligonucleotides are also described. Effectively, the convertible and the constrained approaches have been merged to create constrained and convertible nucleotides (C2NA) providing unique tools to functionalize and stabilize nucleic acids.

The role of molecular structure of sugar-phosphate backbone and nucleic acid bases in the formation of single-stranded and double-stranded DNA structures

Biopolymers ◽  
2014 ◽  
Vol 101 (6) ◽  
pp. 640-650 ◽  
Author(s):  
Valeri Poltev ◽  
Victor M. Anisimov ◽  
Victor I. Danilov ◽  
Dolores Garcia ◽  
Carolina Sanchez ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Nucleic Acid ◽  
Sugar Phosphate ◽  
Nucleic Acid Bases ◽  
Dna Structures ◽  
Double Stranded Dna ◽  
Phosphate Backbone
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