Occurrence of Peanut stripe virus on patchouli and raising of virus-free patchouli plants by meristem tip culture

2009 ◽  
Vol 116 (1) ◽  
pp. 2-6 ◽  
Author(s):  
M. K. Singh ◽  
V. Chandel ◽  
V. Hallan ◽  
R. Ram ◽  
A. A. Zaidi
Keyword(s):  
Peanut Stripe Virus ◽  
Meristem Tip Culture

scholarly journals Virus free of ledfroll infected Koshu vines by meristem tip culture and influence of virus free on compositions of Koshu fruits and wine.

1988 ◽  
Vol 62 (8) ◽  
pp. 1207-1215
Author(s):  
Hideo TOGAWA ◽  
Tomio SHIMURA ◽  
Dai SHIMAZAKI ◽  
Toshio SATO ◽  
Shoji GOTO
Keyword(s):  
Meristem Tip Culture

MERISTEM TIP CULTURE AND HEAT THERAPY FOR PRODUCTION OF APPLE MOSAIC VIRUS FREE PLANTS IN INDIA

1998 ◽  
pp. 135-142 ◽  
Author(s):  
S.V. Bhardwaj ◽  
S.J. Rai ◽  
P.D. Thakur ◽  
A. Handa
Keyword(s):  
Mosaic Virus ◽  
Heat Therapy ◽  
Meristem Tip Culture

HEALTHY IN VITRO PROPAGATION BY MERISTEM TIP CULTURE OF HELLEBORUS NIGER¿S SELECTED CLONE FOR CUT FLOWER

2006 ◽  
pp. 301-310 ◽  
Author(s):  
R. Poupet ◽  
L. Cardin ◽  
A. Henri ◽  
J.P. Onesto
Keyword(s):  
In Vitro Propagation ◽  
Cut Flower ◽  
Meristem Tip Culture ◽  
Helleborus Niger

Virus Elimination by Meristem-Tip Culture

2020 ◽  
pp. 465-477
Author(s):  
Alangar Ishwara Bhat ◽  
Govind Pratap Rao
Keyword(s):  
Virus Elimination ◽  
Meristem Tip Culture

A Comparison of an Enzyme Linked Immunosorbent Assay (ELISA) and Western Blotting for Detection of Peanut Mottle Virus and Peanut Stripe Virus1

1986 ◽  
Vol 13 (2) ◽  
pp. 64-67 ◽  
Author(s):  
J. L. Sherwood ◽  
H. A. Melouk
Keyword(s):  
Western Blotting ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coat Proteins ◽  
Western Blots ◽  
Peanut Stripe Virus ◽  
Dry Milk ◽  
The Difference

Abstract Western blotting was used to detect infections of peanut cv. Tamnut 74 with peanut mottle virus (PMV) and/or peanut stripe virus (PStV). Leaf samples were ground in electrophoresis sample buffer and heated for 5 min at 95 C prior to electrophoresis in 12% polyacrylamide gels. After electrophoresis, proteins were transferred to nitrocellulose sheets at 100V for 45 min. Western blots were performed by first blocking unbound sites on the nitrocellulose with 5% non-fat dry milk in Tris-buffered saline (TBS), pH 7.4 for 30 min, followed by incubation in a 1/200 dilution of PMV and/or PStV antiserum in TBS (the latter antiserum provided by J. W. Demski, U. of GA) for 45 min. This was followed by incubation in protein-A-peroxidase (2 μg/mL in TBS) for 45 min, followed by 4-chloro-1-napthol plus hydrogen peroxide in TBS. As little as 25 ng of either purified PMV or PStV was detected. This was similar to the limits of detection fo the double sandwich enzyme linked immunosorbent assay (ELISA). Because of the difference in migration of the coat proteins of PMV and PStV, both viruses may be detected in plants infected with PMV and PStV. This assay can be performed in approximately 6 h when mini-gels are used for the initial electrophoretic seperation and does not require the antiserum to be fractionated or bound to an enzyme as is the case with ELISA.

scholarly journals In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

2015 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
J.-Roger Bansimba Mukiese ◽  
Aimé Diamuini Ndofunsu ◽  
Freddy Bulubulu ◽  
Alexandre Mbaya Ntumbula ◽  
Sébastien Luyindula Ndiku
Keyword(s):  
Growth Regulators ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Shoot Development ◽  
Media Composition ◽  
Manihot Esculenta Crantz ◽  
Adenine Sulphate ◽  
Meristem Tip Culture ◽  
The Media

<p>Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (<em>Manihot esculenta</em> Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated <em>in vitro</em> in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.</p>

BYMV-FREE CLONES OF EIGHT GLADIOLUS CULTIVARS OBTAINED BY MERISTEM-TIP CULTURE

1986 ◽  
pp. 299-308 ◽  
Author(s):  
A. Bertaccini ◽  
F. Marani
Keyword(s):  
Meristem Tip Culture

scholarly journals Immunocapture-RT-PCR detection of Cassava common mosaic virus in cassava obtained from meristem-tip culture in Paraná state

2011 ◽  
Vol 36 (5) ◽  
pp. 271-275 ◽  
Author(s):  
Jaqueline M. Silva ◽  
Patrícia R. Carnelossi ◽  
Taise Bijora ◽  
Cassiele U. Facco ◽  
Marcelo H.S. Picoli ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Pcr Detection ◽  
Rt Pcr ◽  
Meristem Tip Culture

Production of Cucumber mosaic virus-free chrysanthemums by meristem tip culture

2004 ◽  
Vol 23 (5) ◽  
pp. 469-473 ◽  
Author(s):  
N Verma ◽  
R Ram ◽  
V Hallan ◽  
K Kumar ◽  
A.A Zaidi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Meristem Tip Culture
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