Origin of Secondary Somatic Embryos and Genetic Stability of the Regenerated Plants in Hevea brasiliensis

2017 ◽  
Vol 20 (2) ◽  
pp. 101-116 ◽  
Author(s):  
T. D. Wang ◽  
T. D. Huang ◽  
H. S. Huang ◽  
Y. W. Hua
Keyword(s):  
Genetic Stability ◽  
Hevea Brasiliensis ◽  
Somatic Embryos ◽  
Regenerated Plants ◽  
Secondary Somatic Embryos

scholarly journals Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Masoumeh Asadi-Aghbolaghi ◽  
Beata Dedicova ◽  
Sonali Sachi Ranade ◽  
Kim-Cuong Le ◽  
Farzad Sharifzadeh ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somaclonal Variation ◽  
Ssr Markers ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Large Scale ◽  
Scale Production ◽  
Ms Medium ◽  
Important Species ◽  
Regenerated Plants

Abstract Background Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry. The seeds have low viability, which limits uses for revegetation. Somatic embryogenesis offers an alternative method for obtaining large numbers of plants from limited seed sources. Results A protocol for plant regeneration from somatic embryos of S. pennata was developed. Somatic embryogenesis was induced on Murashige & Skoog (MS) medium supplemented with 3 mg·L–1 2,4-D subsequently shoots were induced on MS medium and supplemented with 5 mg·L–1 zeatin riboside. The highest shoots induction was obtained when embryogenic callus derived from mature embryos (96%) in combination with MS filter-sterilized medium was used from Khuzestan location. The genetic stability of regenerated plants was analysed using ten simple sequence repeats (SSR) markers from S. pennata which showed no somaclonal variation in regenerated plants from somatic embryos of S. pennata. The regenerated plants of S. pennata showed genetic stability without any somaclonal variation for the four pairs of primers that gave the expected amplicon sizes. This data seems very reliable as three of the PCR products belonged to the coding region of the genome. Furthermore, stable expression of GUS was obtained after Agrobacterium-mediated transformation using a super binary vector carried by a bacterial strain LBA4404. Conclusion To our knowledge, the current work is the first attempt to develop an in vitro protocol for somatic embryogenesis including the SSR marker analyses of regenerated plants, and Agrobacterium-mediated transformation of S. pennata that can be used for its large-scale production for commercial purposes.

scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Endangered Medicinal Plant ◽  
Angelica Glauca

Effects of abscisic acid and high concentrations of PEG on Hevea brasiliensis somatic embryos development

Plant Science ◽  
1997 ◽  
Vol 124 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Laurent Linossier ◽  
Philippe Veisseire ◽  
Françoise Cailloux ◽  
Alain Coudret
Keyword(s):  
Abscisic Acid ◽  
Hevea Brasiliensis ◽  
Somatic Embryos ◽  
High Concentrations

scholarly journals In vitro regeneration and somatic embryogenesis in citrus

2015 ◽  
Vol 24 (2) ◽  
pp. 247-262 ◽  
Author(s):  
El Sawy A Mohamed ◽  
Amina Gomaa ◽  
Nancy Danial
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Rt Pcr ◽  
Mother Plants

Better results were obtained when stigma explants of variegated lemon and citron were used. After ten months, somatic embryos developed into plantlets at a frequency ranged from 13.3 for lime to 66.7% for lemon. Virus presence was tested by ELISA and RT?PCR. The results indicated that the plantlets regenerated through somatic embryogenesis are CTV?free. RAPD analysis was used to asses the genetic stability of plantlets as compared to the mother plants. The results indicated that most plantlets belong to the respective mother plants and the polymorphism percentage was genotype and explant?dependant.Plant Tissue Cult. & Biotech. 24(2): 247-262, 2014 (December

THE ORIGIN AND DEVELOPMENT OF SECONDARY SOMATIC EMBRYOS IN BANANA CV. NANJANGUD RASBALE (SILK GP. RASTHALI, AAB)

2010 ◽  
pp. 349-351
Author(s):  
M. Sukhada ◽  
K.Y. Prathibha ◽  
H.D. Soumya ◽  
R.M. Ranganath
Keyword(s):  
Somatic Embryos ◽  
Secondary Somatic Embryos

scholarly journals Micropropagation of Stevia rebaudiana Betroni and assessment of genetic stability of in vitro regenerated plants using inter simple sequence repeat (ISSR) marker

2014 ◽  
Vol 31 (3) ◽  
pp. 249-256 ◽  
Author(s):  
Hemaid Ibrahim Ahemaidan Soliman ◽  
Ehab Mohamed Rabei Metwali ◽  
Omar Abdel-Hakeim Almaghrabi
Keyword(s):  
Genetic Stability ◽  
Simple Sequence Repeat ◽  
Stevia Rebaudiana ◽  
Inter Simple Sequence Repeat ◽  
Issr Marker ◽  
Sequence Repeat ◽  
Regenerated Plants ◽  
Simple Sequence

Morphogenic competence of Vitis rupestris S. secondary somatic embryos with a long culture history

2001 ◽  
Vol 20 (4) ◽  
pp. 279-284 ◽  
Author(s):  
L. Martinelli ◽  
E. Candioli ◽  
D. Costa ◽  
V. Poletti ◽  
N. Rascio
Keyword(s):  
Somatic Embryos ◽  
Vitis Rupestris ◽  
Culture History ◽  
Secondary Somatic Embryos
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