scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

Callus and cell suspension culture of Chelidonium majus L

2014 ◽  
Vol 11 (2) ◽  
pp. 150-154
Author(s):  
S Otgonpurev ◽  
Kh Altantsetseg ◽  
N Tsevegsuren
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Basal Medium ◽  
Ms Medium ◽  
Chelidonium Majus ◽  
Root Explants ◽  
Root Callus ◽  
Murashige Skoog

Chelidonium majus L has long history as a being useful for the treatment of many diseases in Asian and European countries. Aim of this study is to cultivate callus and cell suspension culture in vitro using plant phytohormones. The proliferative capacity was tested on shoot and root explants, cultivated on Murashige-Skoog basal medium testing two auxins: 2,4-diclorphenoxiacetic acid (2.4D) and napheteline acetic acid in combination with cytokinine: kinnetine (K). Calluses were developed on MS medium with 0.5 mg/l Kin, 0.5 mg/l IAA from root explants, as well when added with 0.5 mg/l NAA and 0.5 mg/l Kin from shoot explants. More biomass of cell suspension culture of shoot and root callus was accumulated on MS medium added with 0.1mg/l Kin. DOI: http://dx.doi.org/10.5564/mjas.v11i2.238 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.150-154

scholarly journals The effects of genotypes and media composition on callogenesis, regeneration and cell suspension culture of chamomile (Matricaria chamomilla L.)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11464
Author(s):  
Aqeel Ahmad ◽  
Muhammad Tahir ul Qamar ◽  
Almeera Shoukat ◽  
Mehtab Muhammad Aslam ◽  
Mohsin Tariq ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
In Vitro Regeneration ◽  
Sustainable Production ◽  
Conservation Strategy ◽  
Direct Regeneration ◽  
Ms Medium ◽  
Indirect Regeneration

Background Chamomile is an important herb being used widely for medicinal purposes. Its multitherapeutic, cosmetic, and nutritional values have been established through years of traditional and scientific use and research. Increased use of medicinal plants necessitates rational use as well as sustainable production of such genetic resources. Plant in vitro micro-propagation poses unique opportunities for sustainable production of medicinal herbs, their regrowth and conservation. The present study aimed to investigate the effects of different explants, plant growth regulators (PGRs) combinations and media type on callogenesis, in vitro regeneration and cell suspension of six chamomile genotypes to enhance its sustainable production. Methods The shoot, lateral sprout, and leaf derived explants of six chamomile genotypes including Isfahan, Shiraz, Kazeron, Goral, Sharokashari and Presso were used for direct and indirect regeneration. For indirect regeneration various doses of NAA and kinetin were used to induce calli which were cultured on MS media containing PGRs for direct and indirect regeneration. Later, cell suspension was established and morphological characterization of CrO3 stained cells was carried out using microscopy. Results and Discussion Our findings revealed that the highest callus percentage and callus volume were observed from lateral sprouts and shoots of genotype Isfahan on MS medium containing 1 mg/L NAA and 1 mg/L kinetin. The in vitro regeneration was found to be genotype dependent while 77% and 77.5% was the highest percentage for indirect and direct regeneration, respectively. Additionally, the maximum shoot number (two shoots/explant) and shoot length (2.22 cm) were also observed in Isfahan genotype. Cell suspension culture showed the highest fresh weight (18.59 g) and dry weight (1.707 g) with 0.75 g inoculum of the callus derived from lateral sprouts cultured on MS medium. Microscopy of CrO3 stained cells was carried on each 3rd day for 27 days that revealed larger and spongier cells in the early days as compared to final days when the cell number was greater but cell size was smaller. Conclusion The callogenesis, organogenesis, and cell suspension culture of chamomile may be genotype dependent. Hence, optimization of media ingredients and culture conditions is of utmost importance for devising tissue culture based conservation strategy of any chamomile genotype and secondary metabolite production.

scholarly journals Effect of Plant Growth Regulators on Somatic Embryogenesis and Plantlet Development of Turkey Berry (Solanum torvum SW)

2021 ◽  
pp. 1-8
Author(s):  
Ghan Singh Maloth ◽  
Rajinikanth Marka ◽  
Rama Swamy Nanna
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Solanum Torvum ◽  
Plantlet Formation ◽  
Regenerated Plants

In the present study it was reported on direct somatic embryogenesis and plant regeneration from cotyledon and leaf explants of Turkey berry/pea egg plant (Solanum torvum SW), a medicinally important plant. Somatic embryogenesis has several advantages over other routes of in vitro plant regeneration. Somatic embryogenesis was induced directly from cotyledon and leaf explants on MS medium fortified with BAP (0.5 mg/L)+NAA (0.5-6.0 mg/L). High percentage of somatic embryogenesis (90%), maximum number of somatic embryos formation (62±0.18)  along with high percentage (76%) conversion of somatic embryos into bipolar embryos was observed on cotyledon explants in 0.5 mg/L BAP+2.5 mg/L NAA. At the same concentration of BAP (0.5 mg/L)+NAA (2.5 mg/L) also resulted  on the maximum percentage of somatic embryogenesis (92%), the highest number of somatic embryos formation (88±0.15) and the highest percentage (76%) of somatic embryos conversion into bipolar embryos in leaf explants. A mixture of globular, heart and torpedo-shaped embryos were germinated on MS medium supplemented with 0.5 mg/L IAA+1.0-4.0 mg/L BAP. Maximum germination frequency (75±0.14) of somatic embryos and plantlet formation was found in 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The survival rate of regenerated plants after field transfer was recorded to be 75%. These regenerated plants were found morphologically similar to donor plants. The present protocol can be used for conservation of the species and also for genetic transformation experiments in S. torvum.

scholarly journals VALIDATION OF HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF FOLK LORE KNOWLEDGE OF PURIFIED ANTHOCYANIN FROM CELL SUSPENSION CULTURE OF CLERODENDRON INFORTUNATUM L.: A SEARCH

2017 ◽  
Vol 9 (12) ◽  
pp. 37
Author(s):  
Greeshma Murukan ◽  
S. S. Sumayya ◽  
J. M. Aswathy ◽  
Bosco Lawarence ◽  
K. Murugan
Keyword(s):  
Antioxidant Activity ◽  
Salicylic Acid ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Molar Absorptivity ◽  
Anthocyanin Synthesis ◽  
Ms Medium ◽  
Scavenging Activity

ABSTRACT  Objective: To validate the hepatoprotective and antioxidant activity of purified anthocyanin extracted from the cell suspension culture of Clerodendron infortunatum Linn. Methods: A protocol has been developed for the induction of callus proliferation from leaf and nodal explants of C. infortunatum. The explants were inoculated on MS medium supplemented with diverse combinations of 2,4-D and BAP for triggering callus formation. Subsequently, the green compact callus has been sub-cultured in the medium fortified with 2,4-D and Kinetin for anthocyanin synthesis. Cell suspension culture was also established and the elicitor, salicylic acid was used for triggering anthocyanin synthesis. Three different chromatographic columns (solid phase extraction by Sepharose C18 column, Oasis-MCX and Amberlite XAD 7 + Sephadex LH 120 sorbents) were employed to purify the in vitro synthesized anthocyanin from cell suspension cultures. For purity evaluation HPLC and molar absorptivity assay were used. Further, hepatoprotective and antioxidant activity was evaluated comparing with silymarine, as standard in rats. In vitro antioxidant scavenging activity was analysed by DPPH, FRAP and ORAC assay.Results: After 1 month, the leaf explants yielded remarkable green compact callus on MS medium containing 2.0 mg/l BAP and 0.5 mg/l 2,4-D. Salicylic acid enhanced anthocyanin synthesis. The mean purity values obtained by HPLC were 90.9% ± 1.9 and 80.60% ±2.3 for Oasis MCX, Amberlite XAD-7 + Sephadex LH-20 column respectively. However, the purity calculated by molar absorptivity was found to be less. The highest purity achieved using molar absorptivity analysis was with MCX cartidges i.e., 85.9 ± 3.8%. HPLC yielded 12 anthocyanin fractions. Remarkable antioxidant scavenging activity was noticed as revealed by DPPH, FRAP and ORAC assay. The hepatoprotection activity (25, 50, 100 mg/100g b.w) was compared with silymarine (25 mg/kg b.w) against CCl4 induced toxicity.  Anthocyanin extract improved the AST, ALT and recovered the activity of kidney function by decreasing the urea and creatinine content. In addition, the administration of anthocyanin significantly inhibited the oxidative stress via its scavenging of the reactive oxygen species formed by CCl4 stress. Further, a decrease in the MDA, H2O2, NO accumulation and increase of GSH content were noticed. Similarly,  improved lipid profiles, LDL and HDL levels were also observed suggesting that anthocyanin significantly suppress the toxicity via its activation of antioxidant enzymes (GST, CAT and SOD).Conclusion: The overall results showed that the purified anthocyanin of C. infortunatum function as an antioxidant and  there by hepatoprotective protection against CCl4 inducedtoxicity in animal models. 

scholarly journals Comparison of Purified Anthocyanin Isolated From In Vitro Cell Suspension Culture of Begonia malabarica, Begonia rex-cultorum ‘Baby Rainbow’ and its Antioxidant Activity

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Aswathy J. M. ◽  
Murugan K.
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Hydroxyl Radicals ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Radical Scavenging ◽  
Leaf Explants ◽  
Reducing Power ◽  
Ms Medium

Anthocyanins are the most common flavonoid molecules of vegetables and fruits, especially berries. Human consumption of anthocyanins represents the highest among the flavonoids. Epidemiological studies have suggested that the consumption of anthocyanins lowers the risk of life style disorders like cardiovascular disease, diabetes, arthritis and cancer. Begonia malabarica Lam. of Begoniaceae, is used traditionally as anti-hypoglycemic, antimicrobial, wound healing and in the treatment of anemia. Begonia rex-cultorum ‘Baby rainbow’ an ornamental species was also substituted. Experimentation of in vitro cell suspension culture, isolation, purification of anthocyanin and its antioxidant potential are targeted in the present study. Explants such as leaves and nodes were cultured on MS medium with various phytohormones for callus induction. Leaf explants of Begonia cultured on MS medium fortified with 2, 4-D and BAP showed significant callus induction and also in terms of fresh and dry weights. Significant reddish coloured callus was achieved in cultures initiated from nodal explants in MS medium supplemented with 2, 4-D.Cell suspension cultures were also established in liquid MS medium. After 14 days of culture, cell suspension was obtained with optimal biomass accumulation. Subsequently, bioactive anthocyanin was isolated, purified and fractionated from B.malabarica andB. rex-cultorum‘Baby rainbow’ using amberlite column chromatography and LC-MS/MS analysis. The major anthocyanins eluted from Begonia speciesat 4.7-5.4 min. Tandem MS of the m/z 655.3 peak was identified as anthocyanidin Malvidin-3 –diglucoside as the major compound. The other peaks identified were (584.3) Malvidin or Peonidin, (459.2) Delphinidin + Glucose, (403.2) may be Cynanidin, (287.1) Cyanindin Aglycone and other m/z 242.3, 195.1 and 144.1 were sugar derivatives or fragments. Purified anthocyanin exhibited remarkable inhibition of linoleic acid oxidation and also a concentration dependent free-radical scavenging activities were noticed against DPPH•, hydroxyl radicals and superoxide anions. The degradation of deoxyribose by hydroxyl radicals was also inhibited via iron ion chelators, rather than by directly scavenging the radicals. The results are comparable with reducing power activity of ascorbate and catechin.

scholarly journals INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

2017 ◽  
Vol 16 (1) ◽  
pp. 1-11
Author(s):  
Aryani Leksonowati ◽  
Witjaksono Witjaksono ◽  
Diah Ratnadewi
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Formation ◽  
Combined Treatment ◽  
Cell Suspension Cultures ◽  
Compact Structure ◽  
Friable Callus ◽  
Aquilaria Malaccensis

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage.  The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

scholarly journals Initiation of coconut cell suspension culture from shoot meristem derived embryogenic calli: A preliminary study

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
U. Bhavyashree ◽  
K. Lakshmi Jayaraj ◽  
K. S. Muralikrishna ◽  
K. K. Sajini ◽  
M. K. Rajesh ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Fluorescent Microscopy ◽  
Cell Aggregation ◽  
Shoot Meristem ◽  
Malt Extract ◽  
Embryogenic Calli ◽  
Embryogenic Cell

<p>An attempt was made to establish highly competent embryogenic cell suspension culture in coconut, a species recalcitrant to in vitro culture. Embryogenic calli were initiated from shoot meristem explants of coconut. Y3 medium supplemented with 2.4-D (4.5 μM) and glutamine (34.2 μM) was found to be the best medium to initiate cell suspension. Growth evaluation was done by packed cell volume (PCV) and it was found that maximum growth volume of 9.9% was reached at 200 days of culture initiation. About 52% of viable cells were detected through fluorescent microscopy. Cell aggregation was noticed in Y3 medium supplemented with glutamine (34.2 μM), malt extract (100mg/l), biotin (40.9 μM) and kinetin (9.3 μM), but further progress could not be achieved. It was also observed that embryogenic calli were not of a friable type, but were associated with densely aggregated cells. Because of its hard nature, we were unsuccessful to obtain high quality cell suspension.</p>

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