Analysis of electrophoretic mobility data for biological cells with a new membrane model

Author(s):  
T. Kondo ◽  
H. Ohshima
2020 ◽  
Vol 4 (2) ◽  
pp. 20 ◽  
Author(s):  
Marco Galli ◽  
Szilárd Sáringer ◽  
István Szilágyi ◽  
Gregor Trefalt

Critical coagulation concentration (CCC) is a key parameter of particle dispersions, since it provides the threshold limit of electrolyte concentrations, above which the dispersions are destabilized due to rapid particle aggregation. A computational method is proposed to predict CCC values using solely electrophoretic mobility data without the need to measure aggregation rates of the particles. The model relies on the DLVO theory; contributions from repulsive double-layer forces and attractive van der Waals forces are included. Comparison between the calculated and previously reported experimental CCC data for the same particles shows that the method performs well in the presence of mono and multivalent electrolytes provided DLVO interparticle forces are dominant. The method is validated for particles of various compositions, shapes, and sizes.


1996 ◽  
Vol 182 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Jyh-Ping Hsu ◽  
Sung-Hwa Lin ◽  
Shiojenn Tseng

1991 ◽  
Vol 39 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Hiroyuki Ohshima ◽  
Tamotsu Kondo

1983 ◽  
Vol 36 (6) ◽  
pp. 511 ◽  
Author(s):  
DW Cooper ◽  
PA Woolley ◽  
GM Maynes ◽  
FS Sherman ◽  
WE Poole

An investigation of genetic variation in the electrophoretic mobility of the enzyme a-galactosidase A (EC 3.2.1.22) has been carried out for 33 species of Australian metatherian (marsupial) mammals. The results are compatible with the enzyme being sex-linked in macropodids (kangaroos and wallabies) and probably in dasyurids (marsupial 'mice', etc.), as it is in eutherian (placental) mammals. The results also suggest that the mode of dosage compensation for this locus is the same as for other sexlinked loci in kangaroos, i.e. paternal X inactivation, rather than the random X inactivation system of eutherian mammals. The bearing of the enzyme mobility data on phylogenetic relationships among macropodid species is discussed.


1997 ◽  
Vol 18 (7) ◽  
pp. 1109-1119 ◽  
Author(s):  
George G. Slivinsky ◽  
Wesley C. Hymer ◽  
Johann Bauer ◽  
Dennis R. Morrison

1994 ◽  
Vol 50 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Yoshinori Nakano ◽  
Kimiko Makino ◽  
Hiroyuki Ohshima ◽  
Tamotsu Kondo

Genetics ◽  
1977 ◽  
Vol 87 (4) ◽  
pp. 717-742
Author(s):  
Gary Cobbs ◽  
Satya Prakash

ABSTRACT The relationship between charge changes and electrophoretic mobility changes is investigated experimentally. The charge of several proteins is altered by reaction with small molecules of known structure and the change in electrophoretic mobility is measured. The method of Ferguson plots is used to separate charge and shape components of mobility differences. The average effect of an amino acid charge change on the mobility of the esterase-51.00 allele of Drosophila pseudoobscura is estimated to be 0.046. This estimate is then used to apply the step model of Ohta and Kimura (1973) to electrophoretic mobility data for the esterase-5 locus of D. pseudoobscura and D. miranda. The variation in electrophoretic mobility at this locus was found to be in agreement with the predictions of the step model.


Sign in / Sign up

Export Citation Format

Share Document