scholarly journals Pluripotent stem cells reveal the developmental biology of human megakaryocytes and provide a source of platelets for clinical application

2012 ◽  
Vol 69 (20) ◽  
pp. 3419-3428 ◽  
Author(s):  
Naoya Takayama ◽  
Koji Eto
Keyword(s):  
Stem Cells ◽  
Developmental Biology ◽  
Clinical Application ◽  
Pluripotent Stem Cells

scholarly journals Pluripotent Stem Cells in Developmental Biology

2021 ◽  
Author(s):  
Makoto Ikeya ◽  
Yayoi Toyooka ◽  
Mototsugu Eiraku
Keyword(s):  
Stem Cells ◽  
Developmental Biology ◽  
Pluripotent Stem Cells

Pluripotent Stem Cells in Developmental Biology (Part 2)

2021 ◽  
Author(s):  
Makoto Ikeya ◽  
Yayoi Toyooka ◽  
Mototsugu Eiraku
Keyword(s):  
Stem Cells ◽  
Developmental Biology ◽  
Pluripotent Stem Cells

Induced pluripotent stem cells: developmental biology to regenerative medicine

2010 ◽  
Vol 7 (12) ◽  
pp. 700-710 ◽  
Author(s):  
Timothy J. Nelson ◽  
Almudena Martinez-Fernandez ◽  
Andre Terzic
Keyword(s):  
Stem Cells ◽  
Developmental Biology ◽  
Regenerative Medicine ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals The Opportunities and Challenges regarding Induced Platelets from Human Pluripotent Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Meng-Xue Xu ◽  
Li-Ping Liu ◽  
Yu-Mei Li ◽  
Yun-Wen Zheng
Keyword(s):  
Stem Cells ◽  
Clinical Application ◽  
Pluripotent Stem Cells ◽  
High Efficiency ◽  
Storage Temperature ◽  
Scale Up ◽  
Human Pluripotent Stem Cells ◽  
Scale Production ◽  
Platelet Production ◽  
Platelet Transfusions

As a standard clinical treatment, platelet transfusion has been employed to prevent hemorrhage in patients with thrombocytopenia or platelet dysfunctions. Platelets also show therapeutic potential for aiding liver regeneration and bone healing and regeneration and for treating dermatological conditions. However, the supply of platelets rarely meets the rising clinical demand. Other issues, including short shelf life, strict storage temperature, and allogeneic immunity caused by frequent platelet transfusions, have become serious challenges that require the development of high-yielding alternative sources of platelets. Human pluripotent stem cells (hPSCs) are an unlimited substitution source for regenerative medicine, and patient-derived iPSCs can provide novel research models to explore the pathogenesis of some diseases. Many studies have focused on establishing and modifying protocols for generating functional induced platelets (iPlatelets) from hPSCs. To reach high efficiency production and eliminate the exogenous antigens, media supplements and matrix have been optimized. In addition, the introduction of some critical transgenes, such as c-MYC, BMI1, and BCL-XL, can also significantly increase hPSC-derived platelet production; however, this may pose some safety concerns. Furthermore, many novel culture systems have been developed to scale up the production of iPlatelets, including 2D flow systems, 3D rotary systems, and vertical reciprocal motion liquid culture bioreactors. The development of new gene-editing techniques, such as CRISPR/Cas9, can be used to solve allogeneic immunity of platelet transfusions by knocking out the expression of B2M. Additionally, the functions of iPlatelets were also evaluated from multiple aspects, including but not limited to morphology, structure, cytoskeletal organization, granule content, DNA content, and gene expression. Although the production and functions of iPlatelets are close to meeting clinical application requirements in both quantity and quality, there is still a long way to go for their large-scale production and clinical application. Here, we summarize the diverse methods of platelet production and update the progresses of iPlatelets. Furthermore, we highlight recent advances in our understanding of key transcription factors or molecules that determine the platelet differentiation direction.

FEATURES

2011 ◽  
Vol 15 (07) ◽  
pp. 15-28 ◽  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Drug Discovery ◽  
Regenerative Medicine ◽  
Clinical Application ◽  
Pluripotent Stem Cells ◽  
Cell Biology ◽  
Stem Cell Biology ◽  
Differentiated Products ◽  
Safety Testing

The clinical application of stem cells in hematopoietic disease. Use of pluripotent stem cells and their differentiated products in pharmacological drug discovery and safety testing. Messages from the nucleus: Insights into Aging. inStem: The Institute for Stem Cell Biology and Regenerative Medicine.

scholarly journals Immunologic targeting of CD30 eliminates tumourigenic human pluripotent stem cells, allowing safer clinical application of hiPSC-based cell therapy

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nagako Sougawa ◽  
Shigeru Miyagawa ◽  
Satsuki Fukushima ◽  
Ai Kawamura ◽  
Junya Yokoyama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Therapy ◽  
Clinical Application ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

Large Animal Models for the Clinical Application of Human Induced Pluripotent Stem Cells

2019 ◽  
Vol 28 (19) ◽  
pp. 1288-1298 ◽  
Author(s):  
Xiaoqiang Cong ◽  
Shang-Min Zhang ◽  
Matthew W. Ellis ◽  
Jiesi Luo
Keyword(s):  
Stem Cells ◽  
Animal Models ◽  
Induced Pluripotent Stem Cells ◽  
Clinical Application ◽  
Pluripotent Stem Cells ◽  
Large Animal ◽  
Large Animal Models ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document