Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice

2017 ◽  
Vol 74 (11) ◽  
pp. 2095-2106 ◽  
Author(s):  
Annemarie van Nieuwenhuijze ◽  
James Dooley ◽  
Stéphanie Humblet-Baron ◽  
Jayasree Sreenivasan ◽  
Marije Koenders ◽  
...  
Keyword(s):  
B Cell ◽  
Germinal Center ◽  
Cell Response ◽  
Germinal Center B Cell ◽  
Deficient Mice ◽  
B Cell Response

scholarly journals Lower IgG somatic hypermutation rates during acute dengue virus infection is compatible with a germinal center-independent B cell response

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Elizabeth Ernestina Godoy-Lozano ◽  
Juan Téllez-Sosa ◽  
Gilberto Sánchez-González ◽  
Hugo Sámano-Sánchez ◽  
Andrés Aguilar-Salgado ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
B Cell ◽  
Germinal Center ◽  
Cell Response ◽  
Somatic Hypermutation ◽  
Dengue Virus Infection ◽  
B Cell Response

scholarly journals The memory B cell response to influenza vaccination is impaired in older persons

2021 ◽  
Author(s):  
Edward J Carr ◽  
Adam K Wheatley ◽  
Danika L Hill ◽  
Michelle A Linterman
Keyword(s):  
B Cells ◽  
B Cell ◽  
Germinal Center ◽  
Cell Response ◽  
Older Persons ◽  
Memory B Cells ◽  
Memory B Cell ◽  
Age Related ◽  
Single Cell Rna Sequencing ◽  
B Cell Response

AbstractInfluenza imparts an age-related increase in mortality and morbidity. The most effective countermeasure is vaccination; however, vaccines offer modest protection in older adults. To investigate how ageing impacts the memory B cell response we tracked haemagglutinin specific B cells by indexed flow sorting and single cell RNA sequencing in twenty healthy adults administered the trivalent influenza vaccine. We found age-related skewing in the memory B cell compartment six weeks after vaccination, with younger adults developing haemagglutinin specific memory B cells with an FCRL5+ “atypical” phenotype, showing evidence of somatic hypermutation and positive selection, which happened to a lesser extent in older persons. We confirmed the germinal center ancestry of these FCRL5+ “atypical” memory B cells using scRNASeq from fine needle aspirates of influenza responding human lymph nodes and paired blood samples. Together, this study shows that the aged human germinal center reaction and memory B cell response following vaccination is defective.SummaryImmune responses to vaccination wane with age. Using single cell RNA sequencing of influenza vaccine specific B cells, this study delineates changes in B cell memory generation, antibody mutation and their subsequent selection in older persons.

scholarly journals Distinct germinal center selection at local sites shapes memory B cell response to viral escape

2015 ◽  
Vol 212 (10) ◽  
pp. 1709-1723 ◽  
Author(s):  
Yu Adachi ◽  
Taishi Onodera ◽  
Yuki Yamada ◽  
Rina Daio ◽  
Makoto Tsuiji ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Germinal Center ◽  
Cell Response ◽  
Clonal Selection ◽  
Influenza Virus Infection ◽  
Cross Reactivity ◽  
Viral Escape ◽  
Memory B Cell ◽  
B Cell Response

Respiratory influenza virus infection induces cross-reactive memory B cells targeting invariant regions of viral escape mutants. However, cellular events dictating the cross-reactive memory B cell responses remain to be fully defined. Here, we demonstrated that lung-resident memory compartments at the site of infection, rather than those in secondary lymphoid organs, harbor elevated frequencies of cross-reactive B cells that mediate neutralizing antibody responses to viral escape. The elevated cross-reactivity in the lung memory compartments was correlated with high numbers of VH mutations and was dependent on a developmental pathway involving persistent germinal center (GC) responses. The persistent GC responses were focused in the infected lungs in association with prolonged persistence of the viral antigens. Moreover, the persistent lung GCs supported the exaggerated B cell proliferation and clonal selection for cross-reactive repertoires, which served as the predominant sites for the generation of cross-reactive memory progenitors. Thus, we identified the distinct GC selection at local sites as a key cellular event for cross-reactive memory B cell response to viral escape, a finding with important implications for developing broadly protective influenza vaccines.

IL-6-Deficient Mice Form Granulomas in Murine Schistosomiasis That Exhibit an Altered B Cell Response

1998 ◽  
Vol 188 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Arthur M. Blum ◽  
Ahmed Metwali ◽  
David Elliott ◽  
Jie Li ◽  
Matyas Sandor ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Response ◽  
Deficient Mice ◽  
B Cell Response

scholarly journals Maturation and persistence of the anti-SARS-CoV-2 memory B cell response

2020 ◽  
Author(s):  
Aurélien Sokal ◽  
Pascal Chappert ◽  
Anais Roeser ◽  
Giovanna Barba-Spaeth ◽  
Slim Fourati ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Germinal Center ◽  
Cell Response ◽  
Variable Region ◽  
Memory B Cells ◽  
Memory B Cell ◽  
Antibody Secreting Cell ◽  
Cell Clones ◽  
B Cell Response

AbstractMemory B cells play a fundamental role in host defenses against viruses, but to date, their role have been relatively unsettled in the context of SARS-CoV-2. We report here a longitudinal single-cell and repertoire profiling of the B cell response up to 6 months in mild and severe COVID-19 patients. Distinct SARS-CoV-2 Spike-specific activated B cell clones fueled an early antibody-secreting cell burst as well as a durable synchronous germinal center response. While highly mutated memory B cells, including preexisting cross-reactive seasonal Betacoronavirus-specific clones, were recruited early in the response, neutralizing SARS-CoV-2 RBD-specific clones accumulated with time and largely contributed to the late remarkably stable memory B-cell pool. Highlighting germinal center maturation, these cells displayed clear accumulation of somatic mutations in their variable region genes over time. Overall, these findings demonstrate that an antigen-driven activation persisted and matured up to 6 months after SARS-CoV-2 infection and may provide long-term protection.

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