Molecular evidence for species separation in the water mite Hygrobates nigromaculatus Lebert, 1879 (Acari, Hydrachnidia): evolutionary consequences of the loss of larval parasitism

We describe 16 new species and redescribe six established species in the genus Raoiella—R. argenta sp. nov. Beard, R. australica Womersley, R. bauchani sp. nov. Beard & Ochoa, R. calgoa sp. nov. Beard & Ochoa, R. crebra sp. nov. Beard & Ochoa, R. davisi sp. nov. Beard, R. didcota sp. nov. Beard, R. eugeniae (Mohanasundaram), R. goyderi sp. nov. Ochoa & Beard, R. hallingi sp. nov. Beard, R. illyarrie sp. nov. Beard & Ochoa, R. indica Hirst, R. karri sp. nov. Ochoa & Beard, R. macfarlanei Pritchard & Baker, R. marri sp. nov. Beard & Ochoa, R. pandanae Mohanasundaram, R. pooleyi sp. nov. Beard & Ochoa, R. shimpana Meyer, R. tallerack sp. nov. Beard & Ochoa, R. taronga sp. nov. Beard & Ochoa, R. todtiana sp. nov. Beard & Ochoa, R. wandoo sp. nov. Beard & Ochoa. We discuss molecular evidence of species separation, shared character states among groups of species, and patterns in the additions of leg setae throughout ontogeny that occur in the genus. We provide a key to the known species of Raoiella.

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Evolutionary consequences of intra-patient phage predation on microbial populations

eLife ◽

10.7554/elife.03497 ◽

2014 ◽

Vol 3 ◽

Cited By ~ 73

Author(s):

Kimberley D Seed ◽

Minmin Yen ◽

B Jesse Shapiro ◽

Isabelle J Hilaire ◽

Richelle C Charles ◽

...

Keyword(s):

Natural Course ◽

Genomic Diversity ◽

Microbial Populations ◽

Phage Resistance ◽

Molecular Evidence ◽

Evolutionary Trajectory ◽

Virulence Potential ◽

Stool Samples ◽

Evolutionary Consequences ◽

The Impact

The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen during the natural course of infection within individual patients. We analyzed geographically and temporally disparate cholera patient stool samples from Haiti and Bangladesh and found that phage predation can drive the genomic diversity of intra-patient V. cholerae populations. Intra-patient phage-sensitive and phage-resistant isolates were isogenic except for mutations conferring phage resistance, and moreover, phage-resistant V. cholerae populations were composed of a heterogeneous mix of many unique mutants. We also observed that phage predation can significantly alter the virulence potential of V. cholerae shed from cholera patients. We provide the first molecular evidence for predatory phage shaping microbial community structure during the natural course of infection in humans.

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Molecular evidence for two new species of the Hygrobates fluviatilis-complex from the Balkan Peninsula (Acariformes, Hydrachnidia, Hygrobatidae)

Systematic and Applied Acarology ◽

10.11158/saa.25.9.15 ◽

2020 ◽

Vol 25 (9) ◽

pp. 1702-1719

Author(s):

Vladimir Pešić ◽

Milica Jovanović ◽

Ana Manović ◽

Andrzej Zawal ◽

Aleksandra Bańkowska ◽

...

Keyword(s):

New Species ◽

Balkan Peninsula ◽

Sister Species ◽

Molecular Evidence ◽

Running Waters ◽

Dna Barcodes ◽

Water Mite ◽

Two New Species ◽

Coi Sequences ◽

Taxonomic Approach

Water mites of the Hygrobates fluviatilis-complex are often the most ubiquitous and usually the most abundant water mite group in the running waters of the Palaearctic and Balkans. In the present study we used an integrative taxonomic approach by applying partial COI sequences (DNA-barcodes) to describe two new species of the fluviatilis-complex from the Balkan Peninsula, H. balcanicus Pešić sp. nov. (Bulgaria, North Macedonia, Greece) and H. mediterraneus Pešić sp. nov. (Montenegro). Phylogenetic analysis based on COI data placed H. balcanicus sp. nov. as the sister species of the clade grouping H. turcicus Pešić, Esen & Dabert, 2017 and H. ulii Pešić et al. 2019 whereas H. mediterraneus sp. nov. is placed as the sister species of H. grabowskii Pešić et al. 2019, with about 20% and 13% K2P divergence, respectively, between the closest relatives.

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Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904)

PLoS ONE ◽

10.1371/journal.pone.0241881 ◽

2021 ◽

Vol 16 (3) ◽

pp. e0241881

Author(s):

Samuel Nibouche ◽

Laurent Costet ◽

Raul F. Medina ◽

Jocelyn R. Holt ◽

Joëlle Sadeyen ◽

...

Keyword(s):

Hind Tibia ◽

Molecular Data ◽

Distribution Area ◽

Molecular Evidence ◽

Melanaphis Sacchari ◽

Sugarcane Aphid ◽

The Us ◽

Species Separation ◽

The Caribbean ◽

Morphological Species

Melanaphis sacchari(Zehntner, 1897) andMelanaphis sorghi(Theobald, 1904) are major worldwide crop pests causing direct feeding damage on sorghum and transmitting viruses to sugarcane. It is common in the scientific literature to consider these two species as synonyms, referred to as the ‘sugarcane aphid’, although no formal study has validated this synonymy. In this study, based on the comparison of samples collected from their whole distribution area, we use both morphometric and molecular data to better characterize the discrimination betweenM.sacchariandM.sorghi. An unsupervised multivariate analysis of morphometric data clearly confirmed the separation of the two species. The best discriminating characters separating these species were length of the antenna processus terminalis relative to length of hind tibia, siphunculus or cauda. However, those criteria sometimes do not allow an unambiguous identification. Bayesian clustering based on microsatellite data delimited two clusters, which corresponded to the morphological species separation. The DNA sequencing of three nuclear and three mitochondrial regions revealed slight divergence between species. In particular, the COI barcode region proved to be uninformative for species separation because one haplotype is shared by both species. In contrast, one SNP located on the nuclear EF1-α gene was diagnostic for species separation. Based on morphological and molecular evidence, the invasive genotype damaging to sorghum in the US, Mexico and the Caribbean since 2013 is found to beM.sorghi.

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Morphometric and Molecular Discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904)

10.1101/2020.10.23.351833 ◽

2020 ◽

Author(s):

Samuel Nibouche ◽

Laurent Costet ◽

Raul F. Medina ◽

Jocelyn R. Holt ◽

Joëlle Sadeyen ◽

...

Keyword(s):

Hind Tibia ◽

Molecular Data ◽

Distribution Area ◽

Molecular Evidence ◽

Melanaphis Sacchari ◽

Sugarcane Aphid ◽

The Us ◽

Species Separation ◽

The Caribbean ◽

Morphological Species

AbstractMelanaphis sacchari (Zehntner, 1897) and Melanaphis sorghi (Theobald, 1904) are major worldwide crop pests causing direct feeding damage on sorghum and transmitting viruses to sugarcane. It is common in the scientific literature to consider these two species as synonyms, referred to as the ‘sugarcane aphid’, although no formal study has validated this synonymy. In this study, based on the comparison of samples collected from their whole distribution area, we use both morphometric and molecular data to better characterize the discrimination between M. sacchari and M. sorghi. An unsupervised multivariate analysis of morphometric data clearly confirmed the separation of the two species. The best discriminating characters separating these species were length of the antenna processus terminalis relative to length of hind tibia, siphunculus or cauda. However, those criteria sometimes do not allow an unambiguous identification. Bayesian clustering based on microsatellite data delimited two clusters, which corresponded to the morphological species separation. The DNA sequencing of three nuclear and three mitochondrial regions revealed slight divergence between species. In particular, the COI barcode region proved to be uninformative for species separation because one haplotype is shared by both species. In contrast, one SNP located on the nuclear EF1-α gene was diagnostic for species separation. Based on morphological and molecular evidence, the invasive genotype damaging to sorghum in the US, Mexico and the Caribbean since 2013 is found to be M. sorghi.

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Direct molecular evidence for the expression of the 5-HT4 receptor in rat colonocytes

Gastroenterology ◽

10.1016/s0016-5085(01)80847-7 ◽

2001 ◽

Vol 120 (5) ◽

pp. A171-A171

Author(s):

M STONER ◽

D YAGER ◽

J KELLUM

Keyword(s):

Molecular Evidence

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Water mite parasitism in damselflies during emergence: two hosts, one pattern

Ecography ◽

10.1034/j.1600-0587.2000.d01-1635.x ◽

2000 ◽

Vol 23 (3) ◽

pp. 273-282 ◽

Cited By ~ 1

Author(s):

J. Rolff

Keyword(s):

Water Mite

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A new species of Contomastix (Squamata, Teiidae) supported by total evidence, with remarks on diagnostic characters defining the genus

Herpetological Journal ◽

10.33256/hj29.1.2336 ◽

2019 ◽

pp. 23-36

Author(s):

Mario. R. Cabrera

Keyword(s):

New Species ◽

Geographic Distribution ◽

Phylogenetic Reconstruction ◽

Total Evidence ◽

Molecular Evidence ◽

Proximal Margin ◽

Diagnostic Characters ◽

Morphological Differences ◽

A New Species ◽

Molecular Characters

Formerly Cnemidophorus was thought to be the most speciose genus of Teiidae. This genus comprised four morphological groups that were later defined as four different genera, Ameivula, Aurivela, Cnemidophorus and Contomastix. The last appears as paraphyletic in a recent phylogenetic reconstruction based on morphology, but monophyletic in a reconstruction using molecular characters. Six species are allocated to Contomastix. One of them, C. lacertoides, having an extensive and disjunct geographic distribution in Argentina, Uruguay and Brazil. Preliminary analyses revealed morphological differences among its populations, suggesting that it is actually a complex of species. Here, we describe a new species corresponding to the Argentinian populations hitherto regarded as C. lacertoides, by integrating morphological and molecular evidence. Furthermore, we demonstrate that the presence of notched proximal margin of the tongue is a character that defines the genus Contomastix.

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