Isolation of TIR and nonTIR NBS–LRR resistance gene analogues and identification of molecular markers linked to a powdery mildew resistance locus in chestnut rose (Rosa roxburghii Tratt)

Isolates of Magnaporthe oryzae (the causal agent of rice blast disease) can infect a range of grass species, including barley. We report that barley Hordeum vulgare cv. Baronesse and an experimental line, BCD47, show a range of resistance reactions to infection with two rice blast isolates. The complete resistance of Baronesse to the isolate Ken 54–20 is controlled by a single dominant gene, designated RMo1. RMo1 mapped to the same linkage map position on chromosome 1H as the powdery mildew resistance locus Mla and an expressed sequence tag (k04320) that corresponds to the barley gene 711N16.16. A resistance quantitative trait locus (QTL), at which Baronesse contributed the resistance allele, to the isolate Ken 53–33 also mapped at the same position as RMo1. Synteny analysis revealed that a corresponding region on rice chromosome 5 includes the bacterial blight resistance gene xa5. These results indicate that a defined region on the short arm of barley chromosome 1H, including RMo1 and Mla, harbors genes conferring qualitative and quantitative resistance to multiple pathogens. The partial resistance of BCD47 to Ken53–33 is determined by alleles at three QTL, two of which coincide with the linkage map positions of the mildew resistance genes mlo and Mlf.

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Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine

Theoretical and Applied Genetics ◽

10.1007/s00122-001-0768-1 ◽

2002 ◽

Vol 104 (4) ◽

pp. 610-618 ◽

Cited By ~ 103

Author(s):

T. M. Donald ◽

F. Pellerone ◽

A.-F. Adam-Blondon ◽

A. Bouquet ◽

M. R. Thomas ◽

...

Keyword(s):

Powdery Mildew ◽

Resistance Gene ◽

Powdery Mildew Resistance ◽

Resistance Locus ◽

Resistance Gene Analogs ◽

Mildew Resistance

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Strategies for RUN1 Deployment Using RUN2 and REN2 to Manage Grapevine Powdery Mildew Informed by Studies of Race Specificity

Phytopathology ◽

10.1094/phyto-09-14-0244-r ◽

2015 ◽

Vol 105 (8) ◽

pp. 1104-1113 ◽

Cited By ~ 27

Author(s):

Angela Feechan ◽

Marianna Kocsis ◽

Summaira Riaz ◽

Wei Zhang ◽

David M. Gadoury ◽

...

Keyword(s):

Powdery Mildew ◽

Resistance Gene ◽

Resistance Genes ◽

Powdery Mildew Resistance ◽

Interleukin 1 ◽

Resistance Locus ◽

Mildew Resistance ◽

Grapevine Powdery Mildew ◽

Additional Resistance ◽

Race Specificity

The Toll/interleukin-1 receptor nucleotide-binding site leucine-rich repeat gene, “resistance to Uncinula necator 1” (RUN1), from Vitis rotundifolia was recently identified and confirmed to confer resistance to the grapevine powdery mildew fungus Erysiphe necator (syn. U. necator) in transgenic V. vinifera cultivars. However, sporulating powdery mildew colonies and cleistothecia of the heterothallic pathogen have been found on introgression lines containing the RUN1 locus growing in New York (NY). Two E. necator isolates collected from RUN1 vines were designated NY1-131 and NY1-137 and were used in this study to inform a strategy for durable RUN1 deployment. In order to achieve this, fitness parameters of NY1-131 and NY1-137 were quantified relative to powdery mildew isolates collected from V. rotundifolia and V. vinifera on vines containing alleles of the powdery mildew resistance genes RUN1, RUN2, or REN2. The results clearly demonstrate the race specificity of RUN1, RUN2, and REN2 resistance alleles, all of which exhibit programmed cell death (PCD)-mediated resistance. The NY1 isolates investigated were found to have an intermediate virulence on RUN1 vines, although this may be allele specific, while the Musc4 isolate collected from V. rotundifolia was virulent on all RUN1 vines. Another powdery mildew resistance locus, RUN2, was previously mapped in different V. rotundifolia genotypes, and two alleles (RUN2.1 and RUN2.2) were identified. The RUN2.1 allele was found to provide PCD-mediated resistance to both an NY1 isolate and Musc4. Importantly, REN2 vines were resistant to the NY1 isolates and RUN1REN2 vines combining both genes displayed additional resistance. Based on these results, RUN1-mediated resistance in grapevine may be enhanced by pyramiding with RUN2.1 or REN2; however, naturally occurring isolates in North America display some virulence on vines with these resistance genes. The characterization of additional resistance sources is needed to identify resistance gene combinations that will further enhance durability. For the resistance gene combinations currently available, we recommend using complementary management strategies, including fungicide application, to reduce populations of virulent isolates.

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Genetic map of the powdery mildew resistance gene in soybean PI 243540

Genome ◽

10.1139/g10-015 ◽

2010 ◽

Vol 53 (5) ◽

pp. 400-405 ◽

Cited By ~ 14

Author(s):

Sung-Taeg Kang ◽

M.A. Rouf Mian

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Resistance Gene ◽

Powdery Mildew Resistance ◽

Dominant Gene ◽

Common Disease ◽

Single Dominant Gene ◽

Powdery Mildew Resistance Gene ◽

Chromosome 16 ◽

Mildew Resistance

Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We have discovered a single dominant gene in PI 243540 that provides season-long resistance to powdery mildew. The objective of this study was to map the powdery mildew resistance gene in PI 243540 with PCR-based molecular markers. One hundred eighty-four F2 plants and their F2:3 families from a cross between the powdery mildew susceptible cultivar ‘Wyandot’ and PI 243540 were screened with M. diffusa in greenhouses. Bulked segregant analysis (BSA) with SSR markers was used to identify the tentative genomic location of the gene. The BSA localized the gene to a genomic region in soybean chromosome 16. A linkage map with seven SSR and six SNP markers flanking the gene was constructed. We positioned the gene between SSR marker Sat_224 and SNP marker BARC-021875-04228 at distances of 9.6 and 1.3 cM from the markers, respectively. The map position of the gene was slightly different from previously reported map positions of the only known Rmd locus. We have mapped a single dominant gene, tentatively called Rmd_PI243540, near the previously known Rmd locus on chromosome 16. The molecular markers flanking the gene will be useful for marker-assisted selection of this gene.

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Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

10.1101/698324 ◽

2019 ◽

Author(s):

Huanhuan Li ◽

Zhenjie Dong ◽

Chao Ma ◽

Xiubin Tian ◽

Zhiguo Xiang ◽

...

Keyword(s):

Molecular Markers ◽

Disease Resistance ◽

Powdery Mildew ◽

Resistance Gene ◽

Powdery Mildew Resistance ◽

Disease Resistance Gene ◽

R Genes ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance ◽

Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

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ANALYSIS OF GRAPEVINE HYBRID FAMILY WITH MOLECULAR MARKERS LINKED TO POWDERY MILDEW RESISTANCE GENE

Acta Horticulturae ◽

10.17660/actahortic.2009.827.110 ◽

2009 ◽

pp. 627-630 ◽

Cited By ~ 1

Author(s):

P. Kozma ◽

G. Halász ◽

S. Molnár ◽

ZS. Galbács ◽

S. Hoffmann ◽

...

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Resistance Gene ◽

Powdery Mildew Resistance ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance

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Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

10.20944/preprints201912.0322.v1 ◽

2019 ◽

Author(s):

Zhenjie Dong ◽

Xiubin Tian ◽

Chao Ma ◽

Qing Xia ◽

Beilin Wang ◽

...

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Binding Site ◽

Resistance Gene ◽

Resistance Genes ◽

Powdery Mildew Resistance ◽

Nucleotide Binding ◽

Leucine Rich Repeat ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% of 2Ss#1 segment on the long arm of chromosome 2Ss#1. In this study, we performed RNA-Seq on three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines with Bgt-isolates inoculation at 0, 12, 24, and 48 hours after inoculation. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homoelogous (Ph) genes. Analysis of the 46 ph1b-induced 2Ss#1L recombinants with different Bgt-responses using 28 2Ss#1L-specific molecular markers in the interval FL0.72-0.87 where Pm57 is located, and the flanking intervals, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese spring (T. aestivum L.) with other model species we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for further cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

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