Soybean molecular linkage group B1 corresponds to classical linkage group 16 based on map location of the lf 2 gene

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.

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Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Genome ◽

10.1139/gen-2014-0018 ◽

2014 ◽

Vol 57 (3) ◽

pp. 155-160 ◽

Cited By ~ 9

Author(s):

Yang Yang ◽

Benjamin D. Speth ◽

Napatsakorn Boonyoo ◽

Eric Baumert ◽

Taylor R. Atkinson ◽

...

Keyword(s):

Linkage Group ◽

Male Sterility ◽

Genetic Linkage ◽

Chromosome 2 ◽

Linkage Maps ◽

Male Sterile ◽

Molecular Linkage Group ◽

Sterile Female ◽

Genetic Linkage Maps ◽

Sterility Genes

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

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Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽

10.1139/gen-42-3-453 ◽

1999 ◽

Vol 42 (3) ◽

pp. 453-456 ◽

Cited By ~ 9

Author(s):

Yun Hai Lu ◽

Geneviève Gagne ◽

Bruno Grezes-Besset ◽

Philippe Blanchard

Keyword(s):

Linkage Group ◽

Resistance Gene ◽

Molecular Linkage Group ◽

Rflp Map

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Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽

10.1139/g98-135 ◽

1999 ◽

Vol 42 (3) ◽

pp. 453-456 ◽

Cited By ~ 10

Author(s):

Yun Hai Lu ◽

Geneviève Gagne ◽

Bruno Grezes-Besset ◽

Philippe Blanchard

Keyword(s):

Linkage Group ◽

Linkage Map ◽

Resistance Gene ◽

Scar Marker ◽

Rapd Marker ◽

Rflp Marker ◽

Rflp Markers ◽

Scar Markers ◽

Molecular Linkage Group ◽

Rflp Map

A linkage group containing the Or5 gene conferring resistance to Orobanche cumana race E, as well as 5 SCAR markers and 1 RAPD marker has been recently identified in sunflower. A SCAR marker RTS05, mapped 5.6 cM proximal to the Or5 locus, was analysed in an F2 population for which the segregation data of 80 RFLP markers (GIE cartisol - Phase II, France) were available. An association was found between the SCAR marker RTS05 and an RFLP marker S009 (32.1 cM, LOD = 4.7) that had been mapped to the linkage group 17 of the GIE Cartisol RFLP map. Another RFLP marker S010, tightly linked to S009 (0.0 cM) in the same linkage group, was screened in the F2 population that had been previously used for the Or5 linkage map identification. S010 was found to be significantly linked to all 5 SCAR markers as well as to the single RAPD marker with a LOD > 3.0 in each case. This RFLP marker was mapped between two SCAR markers and was situated at 35.1 cM from the resistance gene with a LOD = 2.7. These results showed that the Or5 linkage group could be integrated with the linkage group 17 of the GIE Cartisol RFLP map.Key words: Helianthus, Orobanche, RFLP, SCAR, linkage map.

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The Pd2 and Lf2 Loci Define Soybean Linkage Group 16

Crop Science ◽

10.2135/cropsci2003.2028 ◽

2003 ◽

Vol 43 (6) ◽

pp. 2028-2030 ◽

Cited By ~ 9

Author(s):

Thomas E. Devine

Keyword(s):

Linkage Group ◽

Group 16

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Positioning 3 Qualitative Trait Loci on Soybean Molecular Linkage Group E

Journal of Heredity ◽

10.1093/jhered/esn070 ◽

2008 ◽

Vol 99 (6) ◽

pp. 674-678 ◽

Cited By ~ 6

Author(s):

R. G. Palmer ◽

M. Xu

Keyword(s):

Linkage Group ◽

Qualitative Trait ◽

Molecular Linkage Group ◽

Trait Loci

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Comparative genomic analysis of sequences sampled from a small region on soybean (Glycine max) molecular linkage group G

Genome ◽

10.1139/g02-027 ◽

2002 ◽

Vol 45 (4) ◽

pp. 634-645 ◽

Cited By ~ 25

Author(s):

Dawn Foster-Hartnett ◽

Joann Mudge ◽

Dana Larsen ◽

Dariush Danesh ◽

Huihuang Yan ◽

...

Keyword(s):

Glycine Max ◽

Linkage Group ◽

Large Scale ◽

Repetitive Sequences ◽

Comparative Genomic ◽

Sequence Comparisons ◽

Cross Hybridization ◽

Molecular Linkage Group ◽

Artificial Chromosomes ◽

Bac Clones

Eight DNA markers spanning an interval of approximately 10 centimorgans (cM) on soybean (Glycine max) molecular linkage group G (MLG-G) were used to identify bacterial artificial chromosome (BAC) clones. Twenty-eight BAC clones in eight distinct contiguous groups (contigs) were isolated from this genome region, along with 59 BAC clones on 17 contigs homoeologous to those on MLG-G. BAC clones in four of the MLG-G contigs were also digested to produce subclones and detailed physical maps. All of the BAC-ends were sequenced, as were the subclones, to estimate proportions in different sequence categories, compare similarities among homoeologs, and explore microsynteny with Arabidopsis. Homoeologous BAC contigs were enriched in repetitive sequences compared with those on MLG-G or the soybean genome as a whole. Fingerprint and cross-hybridization comparisons between MLG-G and homoeologous contigs revealed cases of highly similar physical organization between soybean duplicates, as did DNA sequence comparisons. Twenty-seven out of 78 total sequences on soybean MLG-G showed significant similarity to Arabidopsis. The homologs mapped to six compact genome segments in Arabidopsis, with the longest containing seven homologs spanning two million base pairs. These results extend previous observations of large-scale duplication and selective gene loss in Arabidopsis, suggesting that networks of conserved synteny between Arabidopsis and other angiosperm families can stretch over long physical distances.Key words: Arabidopsis thaliana, bacterial artificial chromosomes, Glycine max, microsynteny.

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