Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Molecular Linkage Group ◽  
Rflp Map

Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Resistance Gene ◽  
Scar Marker ◽  
Rapd Marker ◽  
Rflp Marker ◽  
Rflp Markers ◽  
Scar Markers ◽  
Molecular Linkage Group ◽  
Rflp Map

A linkage group containing the Or5 gene conferring resistance to Orobanche cumana race E, as well as 5 SCAR markers and 1 RAPD marker has been recently identified in sunflower. A SCAR marker RTS05, mapped 5.6 cM proximal to the Or5 locus, was analysed in an F2 population for which the segregation data of 80 RFLP markers (GIE cartisol - Phase II, France) were available. An association was found between the SCAR marker RTS05 and an RFLP marker S009 (32.1 cM, LOD = 4.7) that had been mapped to the linkage group 17 of the GIE Cartisol RFLP map. Another RFLP marker S010, tightly linked to S009 (0.0 cM) in the same linkage group, was screened in the F2 population that had been previously used for the Or5 linkage map identification. S010 was found to be significantly linked to all 5 SCAR markers as well as to the single RAPD marker with a LOD > 3.0 in each case. This RFLP marker was mapped between two SCAR markers and was situated at 35.1 cM from the resistance gene with a LOD = 2.7. These results showed that the Or5 linkage group could be integrated with the linkage group 17 of the GIE Cartisol RFLP map.Key words: Helianthus, Orobanche, RFLP, SCAR, linkage map.

Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Stuart G. Gordon ◽  
Steven K. St. Martin ◽  
Anne E. Dorrance
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Rich Region ◽  
Molecular Linkage Group ◽  
Gene Rich Region

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Fengqun Yu ◽  
Derek J. Lydiate ◽  
S. Roger Rimmer
Keyword(s):  
Brassica Napus ◽  
Linkage Group ◽  
Resistance Gene ◽  
Leptosphaeria Maculans ◽  
Resistance Locus ◽  
Dominant Allele ◽  
Breeding Lines ◽  
Disease Reaction ◽  
Blackleg Resistance ◽  
Map Location

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.

scholarly journals Identification of Cacao TIR-NBS-LRR Resistance Gene Homologues and Their Use as Genetic Markers

2006 ◽  
Vol 131 (6) ◽  
pp. 806-813 ◽  
Author(s):  
David N. Kuhn ◽  
Giri Narasimhan ◽  
Kyoko Nakamura ◽  
J. Steven Brown ◽  
Raymond J. Schnell ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Linkage Group ◽  
Resistance Gene ◽  
Genetic Markers ◽  
Interleukin 1 ◽  
Wrky Transcription Factor ◽  
Candidate Gene Approach ◽  
Degenerate Primers ◽  
Single Strand Conformational Polymorphism ◽  
Group 5

Identifying genetic markers linked to disease resistance in plants is an important goal in marker-assisted selection. Using a candidate-gene approach, we have previously developed genetic markers in cacao (Theobroma cacao L.) for two families of genes involved in disease resistance: non-TIR-NBS-LRR (Toll/Interleukin-1 Receptor-nucleotide binding site-leucine rich repeat) resistance gene homologues and WRKY transcription factor genes; however, we failed to isolate TIR-NBS-LRR genes. Using a novel algorithm to design degenerate primers, we have now isolated TIR-NBS-LRR loci as determined by DNA sequence comparison. These loci have been developed as genetic markers using capillary array electrophoresis (CAE) and single-strand conformational polymorphism (SSCP) analysis. We have mapped three distinct TIR-NBS-LRR loci in an F2 population of cacao and demonstrated that one is located on linkage group 3 and the other two on linkage group 5.

DELINEATION OF A SCAB RESISTANCE GENE CLUSTER ON LINKAGE GROUP 2 OF APPLE

2004 ◽  
pp. 57-62 ◽  
Author(s):  
V. Bus ◽  
W.E. van de Weg ◽  
C.E. Durel ◽  
C. Gessler ◽  
F. Calenge ◽  
...  
Keyword(s):  
Linkage Group ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Scab Resistance ◽  
Resistance Gene Cluster ◽  
Group 2

LINKAGE OF THE GENES FOR DDT AND DIELDRIN RESISTANCE IN LARVAE OF THE MOSQUITO AEDES AEGYPTI

1975 ◽  
Vol 17 (4) ◽  
pp. 543-551 ◽  
Author(s):  
Judith M. Hitchen ◽  
R. J. Wood
Keyword(s):  
Linkage Group ◽  
Aedes Aegypti ◽  
Resistance Gene ◽  
Ddt Resistance ◽  
Group Ii

The DDT resistance gene RDDT1, and the dieldrin resistance gene Rd1 have been mapped on linkage group II with respect to visible markers, in the mosquito Aedes aegypti L. The best interpretation of the data gives the order wa – Rd1 – ds – RDDT1 – s – y but wa – Rd1 – ds – y – s – RDDT1 is also possible, h is very loosely linked with RDDT1. The length of the linkage group has been considerably extended from previous studies.

Molecular mapping of three male-sterile, female-fertile mutants and generation of a comprehensive map of all known male sterility genes in soybean

Genome ◽  
2014 ◽  
Vol 57 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Yang Yang ◽  
Benjamin D. Speth ◽  
Napatsakorn Boonyoo ◽  
Eric Baumert ◽  
Taylor R. Atkinson ◽  
...  
Keyword(s):  
Linkage Group ◽  
Male Sterility ◽  
Genetic Linkage ◽  
Chromosome 2 ◽  
Linkage Maps ◽  
Male Sterile ◽  
Molecular Linkage Group ◽  
Sterile Female ◽  
Genetic Linkage Maps ◽  
Sterility Genes

In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the ms1, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female-fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The ms1 and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to ms1, ms4, and ms6, respectively. The ms1 and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F).

Identification by genome scanning approach (GSA) of a microsatellite tightly associated with the apple scab resistance gene Vm

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 630-636 ◽  
Author(s):  
A Patocchi ◽  
M Walser ◽  
S Tartarini ◽  
G A.L Broggini ◽  
F Gennari ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Simple Sequence Repeat ◽  
Apple Scab ◽  
Scab Resistance ◽  
Sequence Repeat ◽  
Genome Scanning ◽  
Simple Sequence

For all known major apple scab resistance genes except Vr, molecular markers have been published. However, the precise position of some of these genes, in the apple genome, remains to be identified. Knowledge about the relative position of apple scab resistance genes is necessary to preliminarily evaluate the probability of success of their pyramidization. Pyramidization of different resistance genes into the same genotype is a reliable way to create cultivars with durable apple scab resistance. Applying the genome scanning approach (GSA), we identified the linkage group of the scab resistance gene Vm, derived from Malus micromalus, and we found a new molecular marker tightly associated with the gene. The simple sequence repeat Hi07h02, previously mapped on linkage group 17, cosegregates with the Vm gene (no recombinants in the 95 plants tested). The already published sequence-characterized amplified region Vm marker OPB12687 was found to be linked at about 5 cM from the resistance gene and, therefore, this marker also maps on linkage group 17 of apple. This is the first report of the discovery of a major apple scab resistance gene on linkage group 17. The advantages of using GSA for the identification of molecular markers for qualitative traits are discussed.Key words: Malus, Venturia inaequalis, mapping, simple sequence repeat.

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