Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Resistance Gene ◽  
Scar Marker ◽  
Rapd Marker ◽  
Rflp Marker ◽  
Rflp Markers ◽  
Scar Markers ◽  
Molecular Linkage Group ◽  
Rflp Map

A linkage group containing the Or5 gene conferring resistance to Orobanche cumana race E, as well as 5 SCAR markers and 1 RAPD marker has been recently identified in sunflower. A SCAR marker RTS05, mapped 5.6 cM proximal to the Or5 locus, was analysed in an F2 population for which the segregation data of 80 RFLP markers (GIE cartisol - Phase II, France) were available. An association was found between the SCAR marker RTS05 and an RFLP marker S009 (32.1 cM, LOD = 4.7) that had been mapped to the linkage group 17 of the GIE Cartisol RFLP map. Another RFLP marker S010, tightly linked to S009 (0.0 cM) in the same linkage group, was screened in the F2 population that had been previously used for the Or5 linkage map identification. S010 was found to be significantly linked to all 5 SCAR markers as well as to the single RAPD marker with a LOD > 3.0 in each case. This RFLP marker was mapped between two SCAR markers and was situated at 35.1 cM from the resistance gene with a LOD = 2.7. These results showed that the Or5 linkage group could be integrated with the linkage group 17 of the GIE Cartisol RFLP map.Key words: Helianthus, Orobanche, RFLP, SCAR, linkage map.

Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Molecular Linkage Group ◽  
Rflp Map

scholarly journals 085 Development of a Cleaved Amplified pPolymorphic Sequence (CAP) and Restriction Fragment Length Polymorphisms (RFLPs) Linked to the Mi Root-knot Nematode (Meloidogyne incognita, race 1) Resistance Gene in Peach [Prunus persica (L.) Batsch]

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 403C-403
Author(s):  
Anne M. Gillen ◽  
Fredrick A. Bliss
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Rapd Marker ◽  
Rflp Markers ◽  
Resistance Locus ◽  
Root Knot Nematode

Peach rootstock breeding may be accelerated by utilization of molecular markers linked to the root-knot nematode resistance locus (Mi) to screen segregating populations. A genetic linkage map was constructed using RFLP markers in an F2 population (PMP2) that is segregating for this locus. PMP2 is derived from a controlled cross of the relatively diverse peach rootstocks Harrow Blood (susceptible) and Okinawa (homozygous resistant). Bulked Segregant Analysis was applied using RAPD markers. A single small (227 base pairs) RAPD marker was found to be linked to the dominant resistant allele of Mi at a distance of 10 cM. This new marker joined the Mi locus to the RFLP linkage map and showed that two dominant RFLP markers are located between the RAPD marker and Mi. RFLPS are expensive, time-consuming and RAPD markers are unreliable, and therefore both are unsuitable for screening breeding populations. We attempted to convert the RAPD marker to a more breeder-friendly CAPS marker. The converted CAP marker was dominant. Attempts to convert the CAP marker to a co-dominant marker were not successful. The utility of the CAP marker was tested in an open pollinated F2 population derived from the F1 parent of PMP2 and in several rootstocks. The genetic linkage map was compared to other Prunus maps. The PMP2 linkage group containing the Mi locus can be related to the peach × almond linkage group which contains the phosphoglucomutase Pgm-1 locus.

Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Stuart G. Gordon ◽  
Steven K. St. Martin ◽  
Anne E. Dorrance
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Rich Region ◽  
Molecular Linkage Group ◽  
Gene Rich Region

Development and validation of molecular markers linked to an Aegilops umbellulata–derived leaf-rust-resistance gene, Lr9, for marker-assisted selection in bread wheat

Genome ◽  
2005 ◽  
Vol 48 (5) ◽  
pp. 823-830 ◽  
Author(s):  
Sudhir Kumar Gupta ◽  
Ashwini Charpe ◽  
Sunita Koul ◽  
Kumble Vinod Prabhu ◽  
Qazi Mohd. Rizwanul Haq
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Scar Marker ◽  
Rapd Marker ◽  
Gene Pyramiding ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Aegilops Umbellulata

An Aegilops umbellulata–derived leaf-rust-resistance gene, Lr9, was tagged with 3 random amplified polymorphic DNA (RAPD) markers, which mapped within 1.8 cM of gene Lr9 located on chromosome 6BL of wheat. The markers were identified in an F2 population segregating for leaf-rust resistance, which was generated from a cross between 2 near-isogenic lines that differed in the alien gene Lr9 in a widely adopted agronomic background of cultivar 'HD 2329'. Disease phenotyping was done in controlled environmental conditions by inoculating the population with the most virulent pathotype, 121 R63-1 of Puccinia triticina. One RAPD marker, S5550, located at a distance of 0.8 ± 0.008 cM from the Lr9 locus, was converted to sequence-characterized amplified region (SCAR) marker SCS5550. The SCAR marker was validated for its specificity to gene Lr9 against 44 of the 50 known Lr genes and 10 wheat cultivars possessing the gene Lr9. Marker SCS5550 was used with another SCAR marker, SCS73719, previously identified as being linked to gene Lr24 on a segregating F2 population to select for genes Lr9 and Lr24, respectively, demonstrating the utility of the 2 markers in marker-assisted gene pyramiding for leaf-rust resistance in wheat.Key words: wheat, leaf rust resistance, Lr9, Lr24, RAPD, SCAR.

RFLP linkage map and genome analysis of Saccharum spontaneum

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 782-791 ◽  
Author(s):  
Jorge A. G. da Silva ◽  
Mark E. Sorrells ◽  
William L Burnquist ◽  
Steven D. Tanksley
Keyword(s):  
Linkage Map ◽  
Genome Size ◽  
Genome Analysis ◽  
Dna Probes ◽  
Rflp Markers ◽  
Saccharum Spontaneum ◽  
Linkage Groups ◽  
Rflp Map ◽  
Dna Libraries ◽  
Rice Cdna

An RFLP linkage map of the wild sugarcane species Saccharum spontaneum L. (2n = 8x = 40–128) was constructed, comprising 216 loci, detected by 116 DNA probes, and distributed over 44 linkage groups. At a density of at least one marker every 25-cM interval, the coverage of the genome was estimated as 86%. For the generation of RFLP markers, probes were surveyed from seven DNA libraries: three sugarcane cDNA, one oat cDNA, one rice cDNA, and one barley cDNA, as well as one sugarcane genomic. Sixty-two maize genomic clones that were previously mapped on maize were used to initiate a comparative map between the sugarcane, sorghum, and maize genomes. Based on the RFLP segregation data, we conclude that this species is an autopolyploid, with an estimated genome size of 2107 cM.Key words: sugarcane, polyploid, RFLP, map, genome.

scholarly journals Identification and Confirmation of RAPD and SCAR Markers Linked to the ms-3 Gene Controlling Male Sterility in Melon (Cucumis melo L.)

2004 ◽  
Vol 129 (6) ◽  
pp. 819-825 ◽  
Author(s):  
Soon O. Park ◽  
Kevin M. Crosby ◽  
Rongfeng Huang ◽  
T. Erik Mirkov
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Rapd Markers ◽  
Scar Marker ◽  
Rapd Marker ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Scar Markers ◽  
Male Sterile ◽  
The Cross

Male sterility is an important trait of melon in F1 hybrid seed production. Molecular markers linked to a male-sterile gene would be useful in transferring male sterility into fertile melon cultivars and breeding lines. However, markers linked to the ms-3 gene for male sterility present in melon have not been reported. Our objectives were to identify randomly amplified polymorphic DNA (RAPD) markers linked to the ms-3 gene controlling male sterility using bulked segregant analysis in an F2 population from the melon cross of line ms-3 (male-sterile) × `TAM Dulce' (male-fertile), convert the most tightly linked RAPD marker to the ms-3 gene into a sequence characterized amplified region (SCAR) marker based on a specific forward and reverse 20-mer primer pair, and confirm the linkage of the RAPD and SCAR markers with the ms-3 gene in an F2 population from the cross of line ms-3 × `Mission' (male-fertile). A single recessive gene controlling male sterility was found in F2 individuals and confirmed in F3 families. Two RAPD markers that displayed an amplified DNA fragment in the male-sterile bulk were detected to be linked to the ms-3 gene in the F2 population from the cross of line ms-3 × `TAM Dulce'. RAPD marker OAM08.650 was closely linked to the ms-3 gene at 2.1 cM. SCAR marker SOAM08.644 was developed on the basis of the specific primer pair designed from the sequence of the RAPD marker OAM08.650. The linked RAPD and SCAR markers were confirmed in the F2 population from the cross of line ms-3 × `Mission' to be consistently linked to the ms-3 gene at 5.2 cM. These markers were also present in 22 heterozygous fertile F1 plants having the ms-3 gene. The RAPD and SCAR markers linked to the ms-3 gene identified, and confirmed here could be utilized for backcrossing of male sterility into elite melon cultivars and lines for use as parents for F1 hybrid seed production.

An expanded genetic linkage map of Prunus based on an interspecific cross between almond and peach

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 520-529 ◽  
Author(s):  
F A Bliss ◽  
S Arulsekar ◽  
M R Foolad ◽  
V Becerra ◽  
A M Gillen ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Resistance Gene ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Group Identification ◽  
Resistance Gene Analogs ◽  
Linkage Groups ◽  
Genomic Clones ◽  
Trait Loci

The genetic linkage map of Prunus constructed earlier and based on an interspecific F2 population resulting from a cross between almond (Prunus dulcis D.A. Webb) and peach (Prunus persica L. Batsch) was extended to include 8 isozyme loci, 102 peach mesocarp cDNAs, 11 plum genomic clones, 19 almond genomic clones, 7 resistance gene analogs (RGAs), 1 RGA-related sequence marker, 4 morphological trait loci, 3 genes with known function, 4 simple sequence repeat (SSR) loci, 1 RAPD, and 1 cleaved amplified polymorphic sequence (CAP) marker. This map contains 161 markers placed in eight linkage groups that correspond to the basic chromosome number of the genus (x = n = 8) with a map distance of 1144 centimorgans (cM) and an average marker density of 6.8 cM. Four more trait loci (Y, Pcp, D, and SK) and one isozyme locus (Mdh1) were assigned to linkage groups based on known associations with linked markers. The linkage group identification numbers correspond to those for maps published by the Arús group in Spain and the Dirlewanger group in France. Forty-five percent of the loci showed segregation distortion most likely owing to the interspecific nature of the cross and mating system differences between almond (obligate outcrosser) and peach (selfer). The Cat1 locus, known to be linked to the D locus controlling fruit acidity, was mapped to linkage group 5. A gene or genes controlling polycarpel fruit development was placed on linkage group 3, and control of senesced leaf color (in late fall season) (LFCLR) was mapped to linkage group 1 at a putative location similar to where the Y locus has also been placed.Key words: Prunus, molecular markers, RFLPs, resistance gene analogs (RGAs), polycarpel fruit, stone fruits.

Physical Mapping of the liguleless Linkage Group in Sorghum bicolor Using Rice RFLP-Selected Sorghum BACs

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1983-1992
Author(s):  
Michael S Zwick ◽  
M Nurul Islam-Faridi ◽  
Don G Czeschin ◽  
Rod A Wing ◽  
Gary E Hart ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Physical Mapping ◽  
Fluorescent In Situ Hybridization ◽  
Physical Map ◽  
Rflp Markers ◽  
Full Agreement ◽  
Group I ◽  
Chromosome I

Abstract Physical mapping of BACs by fluorescent in situ hybridization (FISH) was used to analyze the liguleless (lg-1) linkage group in sorghum and compare it to the conserved region in rice and maize. Six liguleless-associated rice restriction fragment length polymorphism (RFLP) markers were used to select 16 homeologous sorghum BACs, which were in turn used to physically map the liguleless linkage group in sorghum. Results show a basic conservation of the liguleless region in sorghum relative to the linkage map of rice. One marker which is distal in rice is more medial in sorghum, and another marker which is found within the linkage group in rice is on a different chromosome in sorghum. BACs associated with linkage group I hybridize to chromosome It, which was identified by using FISH in a sorghum cytogenetic stock trisomic for chromosome I (denoted It), and a BAC associated with linkage group E hybridized to an unidentified chromosome. Selected BACs, representing RFLP loci, were end-cloned for RFLP mapping, and the relative linkage order of these clones was in full agreement with the physical data. Similarities in locus order and the association of RFLP-selected BAC markers with two different chromosomes were found to exist between the linkage map of the liguleless region in maize and the physical map of the liguleless region in sorghum.

Linkage map of Cucumis melo including phenotypic traits and sequence-characterized genes

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 761-773 ◽  
Author(s):  
Leah Silberstein ◽  
Irina Kovalski ◽  
Yariv Brotman ◽  
Christophe Perin ◽  
Catherine Dogimont ◽  
...  
Keyword(s):  
Linkage Map ◽  
Resistance Gene ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Simple Sequence Repeats ◽  
Cucumis Melo ◽  
Fragment Length Polymorphism ◽  
Rflp Markers ◽  
Phenotypic Traits ◽  
Simple Sequence

A new linkage map of Cucumis melo, derived from the F2 progeny of a cross between PI 414723 and C. melo 'TopMark' is presented. The map spans a total of 1421 cM and includes 179 points consisting of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSRs), simple sequence repeats (SSRs), and restriction fragment length polymorphism (RFLP) markers. The map also includes an aphid resistance trait (Vat) and the sex type gene, andromonoecious (a), the two of which are important in resistance breeding and the control of hybrid seed production, as well as a seed-color gene, Wt-2. Most RFLPs represent sequence-characterized cDNA probes from C. melo and Cucumis sativus. These include resistance gene homologues and genes involved in various aspects of plant development and metabolism. A sub-set of our SSR and RFLP markers were also mapped, as part of this study, on additional mapping populations that were published for this species. This provides important reference points ("anchors"), enabling us to identify several linkage groups with respect to other melon maps.Key words: Cucumis melo, melon, genetic map, molecular markers, resistance gene homologues.

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