Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

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Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata

Plant Molecular Biology ◽

10.1023/b:plan.0000019068.00034.09 ◽

2003 ◽

Vol 53 (4) ◽

pp. 565-580 ◽

Cited By ~ 46

Author(s):

Yan Wang ◽

Xi Wang ◽

Andrew G. McCubbin ◽

Teh-hui Kao

Keyword(s):

Genetic Mapping ◽

Molecular Characterization ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

Petunia Inflata

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Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai

Czech Journal of Genetics and Plant Breeding ◽

10.17221/159/2012-cjgpb ◽

2013 ◽

Vol 49 (No. 4) ◽

pp. 157-163 ◽

Cited By ~ 1

Author(s):

X. Zhang ◽

C. Ma ◽

D. Yin ◽

W. Zhu ◽

C. Gao ◽

...

Keyword(s):

Brassica Species ◽

Functional Class ◽

S Locus ◽

S Haplotype ◽

Self Compatibility ◽

Female Determinant ◽

First Time ◽

S Locus Glycoprotein ◽

Signalling Cascade

The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

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The Brassica self-incompatibility multigene family

Genome ◽

10.1139/g89-169 ◽

1989 ◽

Vol 31 (2) ◽

pp. 969-972 ◽

Cited By ~ 23

Author(s):

Kathleen G. Dwyer ◽

Anna Chao ◽

Betty Cheng ◽

Che-Hong Chen ◽

June B. Nasrallah

Keyword(s):

Genomic Dna ◽

Genomic Organization ◽

Genetic Locus ◽

Brassica Species ◽

Self Incompatibility ◽

S Locus ◽

S Gene ◽

Gene Copies ◽

Genomic Regions

The pollen–stigma interaction of self-incompatibility in Brassica species is controlled by a single genetic locus, the S locus. This locus encodes the S locus specific glycoproteins of the stigma. Hybridization of restriction enzyme digested genomic DNA isolated from homozygous strains of Brassica with cDNA probes encoding these glycoproteins yields a pattern of multiple fragments of varying size and intensity. The presence of S sequences as multiple related copies in the Brassica oleracea genome has been verified by the cloning of several different genomic regions containing S homology. Probes capable of distinguishing between S gene copies have been obtained and have demonstrated that two such copies are expressed. Characterization of additional probes specific for S gene copies will permit the study of the expression of the other copies, and the analysis of the genomic organization of the self-incompatibility multigene family.Key words: self-incompatibility, Brassica, cDNA probes.

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Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci.

Genetics ◽

10.1093/genetics/127.1.221 ◽

1991 ◽

Vol 127 (1) ◽

pp. 221-228 ◽

Cited By ~ 1

Author(s):

D C Boyes ◽

C H Chen ◽

T Tantikanjana ◽

J J Esch ◽

J B Nasrallah

Keyword(s):

Brassica Oleracea ◽

Fragment Length Polymorphism ◽

Genetic Relationships ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

Related Sequence ◽

Slg Gene ◽

S Locus Glycoprotein

Abstract Self-incompatibility in Brassica oleracea is controlled by the highly polymorphic S locus. Isolation and subsequent characterization of the S-locus-glycoprotein (SLG) gene, which encodes the S-locus-specific glycoprotein (SLSG), has revealed the presence of a self-incompatibility multigene family. One of these S-locus-related genes, SLR1, has been shown to be expressed. In this study we present the isolation and preliminary characterization of a second expressed S-locus-related sequence, SLR2. Through restriction fragment length polymorphism (RFLP) linkage analysis we demonstrate that the SLR1 and SLR2 loci reside approximately 18.5 map units apart in one linkage group that segregates independently of the S-locus. The identification of a second SLR gene expressed in stigmas suggests that loci unlinked to the S-locus may play a role in the self-incompatibility response, or in pollination in general.

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Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars

International Journal of Horticultural Science ◽

10.31421/ijhs/20/3-4/1128 ◽

2014 ◽

Vol 20 (3-4) ◽

Cited By ~ 3

Author(s):

J. Halász ◽

A. Kurilla ◽

A. Hegedűs

Keyword(s):

Genetic Diversity ◽

Unknown Origin ◽

The Self ◽

Self Incompatibility ◽

S Locus ◽

Prunus Domestica ◽

High Genetic Diversity ◽

First Intron ◽

S Alleles

European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the second intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.

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Intrahaplotype Polymorphism at the Brassica S Locus

Genetics ◽

10.1093/genetics/159.2.811 ◽

2001 ◽

Vol 159 (2) ◽

pp. 811-822

Author(s):

Christine Miege ◽

Véronique Ruffio-Châble ◽

Mikkel H Schierup ◽

Didier Cabrillac ◽

Christian Dumas ◽

...

Keyword(s):

Class Ii ◽

The Self ◽

Self Incompatibility ◽

Receptor Kinase ◽

S Locus ◽

S Haplotype

Abstract The S locus receptor kinase and the S locus glycoproteins are encoded by genes located at the S locus, which controls the self-incompatibility response in Brassica. In class II self-incompatibility haplotypes, S locus glycoproteins can be encoded by two different genes, SLGA and SLGB. In this study, we analyzed the sequences of these genes in several independently isolated plants, all of which carry the same S haplotype (S2). Two groups of S2 haplotypes could be distinguished depending on whether SRK was associated with SLGA or SLGB. Surprisingly, SRK alleles from the two groups could be distinguished at the sequence level, suggesting that recombination rarely occurs between haplotypes of the two groups. An analysis of the distribution of polymorphisms along the S domain of SRK showed that hypervariable domains I and II tend to be conserved within haplotypes but to be highly variable between haplotypes. This is consistent with these domains playing a role in the determination of haplotype specificity.

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A Flower-Specific Phospholipase D1 is a Stigmatic Compatibility Factor Targeted by the Self-Incompatibility Response in Brassica Napus

SSRN Electronic Journal ◽

10.2139/ssrn.3195284 ◽

2018 ◽

Cited By ~ 1

Author(s):

Sabine Scandola ◽

Marcus A. Samuel

Keyword(s):

Brassica Napus ◽

The Self ◽

Self Incompatibility ◽

Compatibility Factor ◽

Phospholipase D1

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Molecular characterization of the S locus in two self-incompatible Brassica napus lines.

The Plant Cell ◽

10.1105/tpc.8.12.2369 ◽

1996 ◽

Vol 8 (12) ◽

pp. 2369-2380 ◽

Cited By ~ 33

Author(s):

K Yu ◽

U Schafer ◽

T L Glavin ◽

D R Goring ◽

S J Rothstein

Keyword(s):

Brassica Napus ◽

Molecular Characterization ◽

S Locus

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A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Nature Communications ◽

10.1038/s41467-021-24266-7 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ling Ma ◽

Chunzhi Zhang ◽

Bo Zhang ◽

Fei Tang ◽

Futing Li ◽

...

Keyword(s):

Potato Breeding ◽

Inbred Lines ◽

Hybrid Breeding ◽

Self Incompatibility ◽

Diploid Hybrid ◽

Food Crop ◽

Global Food Security ◽

The Third ◽

Self Compatibility ◽

Global Food

AbstractPotato is the third most important staple food crop. To address challenges associated with global food security, a hybrid potato breeding system, aimed at converting potato from a tuber-propagated tetraploid crop into a seed-propagated diploid crop through crossing inbred lines, is under development. However, given that most diploid potatoes are self-incompatible, this represents a major obstacle which needs to be addressed in order to develop inbred lines. Here, we report on a self-compatible diploid potato, RH89-039-16 (RH), which can efficiently induce a mating transition from self-incompatibility to self-compatibility, when crossed to self-incompatible lines. We identify the S-locusinhibitor (Sli) gene in RH, capable of interacting with multiple allelic variants of the pistil-specific S-ribonucleases (S-RNases). Further, Sli gene functions like a general S-RNase inhibitor, to impart SC to RH and other self-incompatible potatoes. Discovery of Sli now offers a path forward for the diploid hybrid breeding program.

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