Conservation of S-locus for self-incompatibility in Brassica napus (L.) and Brassica oleracea (L.)

1997 ◽  
Vol 95 (1-2) ◽  
pp. 73-82 ◽  
Author(s):  
W. Y. Cheung ◽  
G. Champagne ◽  
N. Hubert ◽  
L. Tulsieram ◽  
D. Charne ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Self Incompatibility ◽  
S Locus ◽  
Brassica Napus L ◽  
Brassica Oleracea L

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

scholarly journals ADUBAÇÃO FOLIAR DE BORO EM DOIS HÍBRIDOS DE CANOLA

Nativa ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 600-604
Author(s):  
Anderson Bergamasco Hryczyna ◽  
Tiago Roque Benetoli da Silva ◽  
Lucas Ambrosano ◽  
Gesmila Karoline Zampronio
Keyword(s):  
Brassica Napus ◽  
Brassica Oleracea ◽  
Brassica Rapa ◽  
Pollen Grain ◽  
Brassica Napus L ◽  
Cell Wall Development ◽  
Leaf Boron ◽  
Boron Fertilization ◽  
Brassicaceae Family ◽  
Brassica Oleracea L

A canola (Brassica napus L. var oleífera) é uma oleaginosa, pertencente à família Brassicaceae, resultado do cruzamento interespecífico entre repolho silvestre (Brassica oleracea L.) e mostarda (Brassica rapa L., syn. campestris). O boro é considerado um micronutriente, que atua na translocação de açúcares, formação de proteínas, crescimento do tubo polínico, germinação dos grãos de pólen, desenvolvimento das sementes e das paredes celulares. O objetivo desta pesquisa foi estudar as interferências nos componentes produtivos de dois híbridos de canola com a aplicação de adubação com boro por via foliar. Para tanto, instalou-se um experimento em condições de campo num delineamento experimental de blocos ao acaso em arranjo fatorial 2 x 3. Utilizou-se os híbridos Hyola 433 e Hyola 575 CL e três doses de boro foliar (0;100; 200) g ha-1 de B. As seguintes características foram avaliadas:  número de plantas, número de síliquas por planta, massa de parte aérea, massa de 100 grãos e produtividade. Com a aplicação de boro houve aumento da massa de grãos independente do híbrido. Para produtividade houve aumento somente para o híbrido Hyola 433, sendo a maior produtividade alcançada na dose de 200 g ha-1. O híbrido Hyola 575 CL não teve sua produtividade alterada com a aplicação de boro. Palavras-chave: ácido bórico; Brassica napus L. var. oleífera; colza; massa de 100 grãos; oleaginosa.   Leaf boron fertilization in two rapessed   ABSTRACT: Rapessed (Brassica napus L. var oleífera) is an oilseed, belongs to Brassicaceae family, resultated between inter specific crossing of wild cabbage (Brassica oleracea L.) and mustard (Brassica rapa L., syn. campestris). Boron is considered a micronutrient, acting in sugars translocation, protein formation, pollen tube growth, pollen grain germination, seeds and cell wall development. The aimed was to study the interferences in productive components of two rapessed hybrids with application of boron fertilization by leaf application. For this, a field experiment was installed by using experimental design of randomized blocks in a 2 x 3 factorial scheme.  Hyola 433 and Hyola 575CL hybrids and three doses of leaf boron (0; 100 and 200) g ha-1 of B were used. The following characteristics were evaluated: plants number, number of pods per plant, mass of one hundred grains and yield. With boron application there was an increase in grain mass independent of the hybrid. In relation grain yield there was increase only for Hyola 433 hybrid, with the highest yield reached at 200 g ha-1. The Hyola 575CL hybrid did not have its productivity altered with the application of boron. Keywords: boric acid; Brassica napus L. var. oleífera; mustard; mass of a hundred grass; oilseeds.

Development of SCAR markers linked to self-incompatibility in Brassica napus L.

2007 ◽  
Vol 21 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Xingguo Zhang ◽  
Chaozhi Ma ◽  
Tingdong Fu ◽  
Yuanyuan Li ◽  
Tonghua Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Scar Markers ◽  
Self Incompatibility ◽  
Brassica Napus L

scholarly journals A Simple Procedure for Mesophyll Protoplast Culture and Plant Regeneration in Brassica oleracea L. and Brassica napus L.

2011 ◽  
Vol 42 (No. 3) ◽  
pp. 103-110 ◽  
Author(s):  
N.D. Kaur ◽  
M. Vyvadilová ◽  
M. Klíma ◽  
M. Bechyně
Keyword(s):  
Brassica Napus ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Growth Regulators ◽  
Protoplast Culture ◽  
Treatment Time ◽  
Simple Procedure ◽  
Enzyme Treatment ◽  
Induction Medium ◽  
Brassica Napus L

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8&ndash;11.2 &times; 10<sup>4 </sup>protoplasts/ml in darkness at 25&deg;C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7<sup>th</sup> and 11<sup>th</sup> day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5&ndash;1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA<sub>3</sub>, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69&ndash;75% in B. oleracea and 2&ndash;3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments. &nbsp;

Test of Interaction Between SRK and THL1 from Brassica oleracea L. in Self-Incompatibility Signaling Process

2008 ◽  
Vol 34 (6) ◽  
pp. 934-943
Author(s):  
Qi-Guo GAO ◽  
Ming SONG ◽  
Yi NIU ◽  
Kun YANG ◽  
Li-Quan ZHU ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Self Incompatibility ◽  
Signaling Process ◽  
Brassica Oleracea L
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