scholarly journals The quest to make fully functional human pancreatic beta cells from embryonic stem cells: climbing a mountain in the clouds

Diabetologia ◽  
2016 ◽  
Vol 59 (10) ◽  
pp. 2047-2057 ◽  
Author(s):  
James D. Johnson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Embryonic Stem

Stimulation of Embryonic Stem Cells to Differentiate into Pancreatic Beta-Cells by Co-axial Method with Momordica charantia L.

2010 ◽  
Vol 46 (0) ◽  
Author(s):  
Maria Gertrude C Derikito ◽  
Maria Wartenberg ◽  
Heinrich Sauer ◽  
Cynthia P Saloma ◽  
Ameurfina D Santos
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Embryonic Stem ◽  
Momordica Charantia ◽  
Stimulation Of

scholarly journals Differentiation of mouse nuclear transfer embryonic stem cells into functional pancreatic beta cells

Diabetologia ◽  
2008 ◽  
Vol 51 (9) ◽  
pp. 1671-1679 ◽  
Author(s):  
W. Jiang ◽  
Z. Bai ◽  
D. Zhang ◽  
Y. Shi ◽  
J. Yong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Nuclear Transfer ◽  
Embryonic Stem

scholarly journals From Core Set to Assemblage

2007 ◽  
Vol 20 (1) ◽  
pp. 5-25
Author(s):  
Mike Michael ◽  
Steven Wainwright ◽  
Clare Williams ◽  
Bobbie Farsides ◽  
Alan Cribb
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Key Words ◽  
Beta Cells ◽  
Embryonic Stem ◽  
Diabetes Treatment ◽  
Core Set ◽  
Assemblage Analysis ◽  
Social Associations

**From Core Set to Assemblage: On the Dynamics of Exclusion and Inclusion in the Failure to Derive Beta Cells from Embryonic Stem Cells** In this paper, we examine the controversy surrounding the Lumelsky protocol (which potentially could have transformed the procedures for differentiating embryonic stem cells into beta cells for diabetes treatment). The protocol is analyzed initially using Collins’ core set model to show how the controversy over epistemic claims was resolved (and the Lumelsky protocol deemed to be a failure). This approach is then contrasted to an analysis in terms of scientific ‘assemblages’ characterized not by the resolution of epistemic controversy, but by the ‘irresolution’ or openness of social associations amongst scientists. We suggest that scientists who jumped on the ‘Lumelsky bandwagon’ can be rehabilitated, partly because of the recognized chronic uncertainty in the stem cell fi eld. Thus, alongside the judgement, resolution and exclusion mapped by core set analysis, there is ‘understanding’, irresolution and inclusion suggested by ‘assemblage analysis’. *Key words*: core set, assemblage, stem cells

scholarly journals Dual Inhibition of BMP and WNT Signals Promotes Pancreatic Differentiation from Human Pluripotent Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Mengtian Tan ◽  
Lai Jiang ◽  
Yinglei Li ◽  
Wei Jiang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
Signal Pathways ◽  
Pancreatic Differentiation ◽  
Dual Inhibition ◽  
Induced Pluripotent ◽  
Wnt Signal

Pathological or functional loss of pancreatic beta cells is the cause of diabetes. Understanding how signaling pathways regulate pancreatic lineage and searching for combinations of signal modulators to promote pancreatic differentiation will definitely facilitate the robust generation of functional beta cells for curing hyperglycemia. In this study, we first tested the effect of several potent BMP inhibitors on pancreatic differentiation using human embryonic stem cells. Next, we examined the endodermal lineage bias upon potent BMP inhibitor treatment and further checked the crosstalk between signal pathways governing endodermal lineage determination. Furthermore, we improved current pancreatic differentiation system based on the signaling pathway study. Finally, we used human-induced pluripotent stem cells to validate our finding. We found BMP inhibitors indeed not only blocked hepatic lineage but also impeded intestinal lineage from human definitive endoderm unexpectedly. Signaling pathway analysis indicated potent BMP inhibitor resulted in the decrease of WNT signal activity and inhibition of WNT could contribute to the improved pancreatic differentiation. Herein, we combined the dual inhibition of BMP and WNT signaling and greatly enhanced human pancreatic progenitor differentiation as well as beta cell generation from both embryonic stem cells and induced pluripotent stem cells. Conclusively, our present work identified the crosstalk between the BMP and WNT signal pathways during human endoderm patterning and pancreas specification, and provided an improved in vitro pancreatic directed differentiation protocol from human pluripotent stem cells.

Chemicals turn human embryonic stem cells towards beta cells

2009 ◽  
Vol 5 (4) ◽  
pp. 195-196 ◽  
Author(s):  
Yu-Ping Yang ◽  
Chris Wright
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals Insulin expressing cells from differentiated embryonic stem cells are not beta cells

Diabetologia ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 499-508 ◽  
Author(s):  
S. Sipione ◽  
A. Eshpeter ◽  
J. G. Lyon ◽  
G. S. Korbutt ◽  
R. C. Bleackley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Embryonic Stem

scholarly journals High dosage of Exendin-4 increased early insulin secretion in differentiated beta cells from mouse embryonic stem cells

2010 ◽  
Vol 31 (5) ◽  
pp. 570-577 ◽  
Author(s):  
Hua Li ◽  
Amy Lam ◽  
Ai-min Xu ◽  
Karen Sl Lam ◽  
Sookja Kim Chung
Keyword(s):  
Stem Cells ◽  
Insulin Secretion ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Embryonic stem cells to beta-cells by understanding pancreas development

2008 ◽  
Vol 288 (1-2) ◽  
pp. 86-94 ◽  
Author(s):  
Marie Best ◽  
Michael Carroll ◽  
Neil A. Hanley ◽  
Karen Piper Hanley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Beta Cells ◽  
Embryonic Stem ◽  
Pancreas Development
Sign in / Sign up

Export Citation Format

Share Document