A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila

2000 ◽  
Vol 173 (4) ◽  
pp. 307-310 ◽  
Author(s):  
Sun N. Wai ◽  
Yoshimitsu Mizunoe ◽  
Akemi Takade ◽  
Shin-ichi Yoshida
Keyword(s):  
Low Temperature ◽  
Aeromonas Hydrophila ◽  
Liquid Media ◽  
Nonculturable Cells

Bioproduction of ochratoxin A and penicillic acid by members of the Aspergillus ochraceus group

1972 ◽  
Vol 18 (5) ◽  
pp. 631-636 ◽  
Author(s):  
Alex Ciegler
Keyword(s):  
Quantitative Analysis ◽  
Low Temperature ◽  
Ochratoxin A ◽  
Acid Synthesis ◽  
Aspergillus Ochraceus ◽  
Cereal Grains ◽  
Liquid Media ◽  
Penicillic Acid ◽  
Group A ◽  
Fluorescent Derivatives

Various strains of species belonging to the Aspergillus ochraceus group (A. ochraceus, A. sclerotiorum, A. alliaceus, A. ostianus, A. melleus, and A. sulphureus) can produce two mycotoxins, ochratoxin A and penicillic acid, on liquid media and in cereal grains. The quantity of each toxin produced is influenced by temperature; low temperature (10 and 20C) favor penicillic acid synthesis and higher (28C), ochratoxin A production. Generally penicillic acid is produced in yields about one to three magnitudes greater than ochratoxin A. A simple fluorodensitometric method for concomitant quantitative analysis of the two toxins has been developed based on conversion of penicillic acid and ochratoxin A to fluorescent derivatives by treatment with ammonia fumes.

scholarly journals Detection of Aeromonas hydrophila in Liquid Media by Volatile Production Similarity Patterns, Using a FF-2A Electronic Nose

Sensors ◽  
2013 ◽  
Vol 13 (1) ◽  
pp. 736-745 ◽  
Author(s):  
Kouki Fujioka ◽  
Eiji Arakawa ◽  
Jun-ichi Kita ◽  
Yoshihiro Aoyama ◽  
Yoshinobu Manome ◽  
...  
Keyword(s):  
Electronic Nose ◽  
Aeromonas Hydrophila ◽  
Liquid Media ◽  
Volatile Production

Low temperature hydrogenation of carbon dioxide into formaldehyde in liquid media

2018 ◽  
Vol 309 ◽  
pp. 242-247 ◽  
Author(s):  
Fan Liang Chan ◽  
Garen Altinkaya ◽  
Nicholas Fung ◽  
Akshat Tanksale
Keyword(s):  
Carbon Dioxide ◽  
Low Temperature ◽  
Liquid Media ◽  
Hydrogenation Of Carbon Dioxide

Numerical taxonomy of psychrotrophic bacteria isolated from raw ewes' milk

1993 ◽  
Vol 60 (3) ◽  
pp. 371-383 ◽  
Author(s):  
María-Rosario García-Armesto ◽  
Miguel Prieto ◽  
Carlos Alonso ◽  
María-Luisa GarcíLópez ◽  
María-Camino García-Fernández ◽  
...  
Keyword(s):  
Low Temperature ◽  
Aeromonas Hydrophila ◽  
Numerical Taxonomy ◽  
Taxonomic Study ◽  
Coagulase Negative Staphylococci ◽  
Gram Negative ◽  
Identification Schemes ◽  
Psychrotrophic Bacteria ◽  
Numerical Taxonomic Study ◽  
Reference Strains

SummaryA total of 204 psychrotrophic isolates from raw ewes' milk (hand and machine milked) were identified by conventional methods. In addition, a numerical taxonomic study was conducted on 180 of these isolates and 19 reference strains. Three of the isolates were yeasts. Using identification schemes, 54 isolates were assigned to genera of Gram-negative aerobic rods (Pseudomonas, Acinetobacter, Flavobacterium, MoraxellaandPsychrobacter), 48 were Enterobacteriaceae (Entero-bacter, Hafnia, Klebsiella, CitrobacterandSerratia) and one was identified asAeromonas hydrophila. The 98 Gram-positive isolates were identified asEnterococcus, Streptococcus, Leuconostoc, Lactococcus, Bacillus, Staphylococcus, Micrococcus, Aureobacterium, KurthiaandMicrobacterium. At the 82% similarity level (SSM), 18 clusters were formed. Cluster I included 34 strainsof Lactococcus, StreptococcusandLeuconostoc. Most of the 35 strains in cluster II wereEnterococcus. Clusters III and IV were identified asKurthiaandMicrobacteriumrespectively. Cluster V was identified asAureobacteriumand cluster VI consisted of coagulase-negative staphylococci. Gram-negative isolates formed 12 clusters:Aeromonas(one cluster), Enterobacteriaceae (two clusters),Flavobacterium(two clusters),PseudomonasandPsychrobacter immobilis(three clusters) andAcinetobacter(four clusters). Non-motile variants ofPs. fragiwere found.Enterococcusand Enterobacteriaceae did not have significant spoilage properties. As expected, Gram-negative aerobic rods were proteolytic and/or lipolytic even at low temperature. Contamination with certain types of psychrotrophs (Gram-negative aerobic rods and enterococci) seemed to be associated with the milking method. The isolate ofAes. hydrophilahad properties associated with virulence.

scholarly journals Resuscitation of viable but nonculturable cells ofVibrio parahaemolyticusinduced at low temperature under starvation

2000 ◽  
Vol 186 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Yoshimitsu Mizunoe ◽  
Sun Nyunt Wai ◽  
Takahiko Ishikawa ◽  
Akemi Takade ◽  
Shin-ichi Yoshida
Keyword(s):  
Low Temperature ◽  
Viable But Nonculturable ◽  
Nonculturable Cells

Low-temperature cyaniding of structural steels in liquid media

1968 ◽  
Vol 10 (4) ◽  
pp. 257-260 ◽  
Author(s):  
V. V. Bogdanov ◽  
Yu. M. Lakhtin ◽  
G. N. Neustroev ◽  
A. I. Ryazanova
Keyword(s):  
Low Temperature ◽  
Structural Steels ◽  
Liquid Media

The effects of different acids on the preparation of TiO2 nanostructure in liquid media at low temperature

2008 ◽  
Vol 111 (2-3) ◽  
pp. 313-316 ◽  
Author(s):  
Zhenyao Wang ◽  
Dingguo Xia ◽  
Ge Chen ◽  
Tao Yang ◽  
Yu Chen
Keyword(s):  
Low Temperature ◽  
Liquid Media ◽  
Tio2 Nanostructure

Effect of chilling on the survival of Bacteroides fragilis

1976 ◽  
Vol 4 (5) ◽  
pp. 432-436
Author(s):  
J C Hagen ◽  
W S Wood ◽  
T Hashimoto
Keyword(s):  
Low Temperature ◽  
Bacteroides Fragilis ◽  
Early Growth ◽  
Anaerobic Conditions ◽  
Liquid Media ◽  
Factors Affecting ◽  
Cold Tolerant ◽  
Agar Media ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Factors affecting the susceptibility of Bacteroides fragilis subsp. fragilis to low temperature were examined. Predetermined numbers of cells were spread on agar media or suspended in enriched Trypticase soy broth and exposed to low temperature under both aerobic and anaerobic conditions. Exposure of 18-h growth of a freshly isolated B. fragilis strain to 4 degrees C aerobically or anaerobically resulted in a loss of at least 50% viability after 12 h. B. fragilis cells in early growth (6 h) were more tolerant to exposure at 4 degrees C than older cells (18 h). When the freshly isolated strain was repeatedly subcultured in the laboratory it was uniformly more cold tolerant than fresh clinical isolates. The incorporation of 1.0 M sucrose and 5 mM magnesium chloride into liquid media partially alleviated the lethal effects of cold temperature on B. fragilis subsp. fragilis.

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