DNA breaks and cell cycle arrest induced by okadaic acid in Caco-2 cells, a human colonic epithelial cell line

2001 ◽  
Vol 75 (2) ◽  
pp. 110-117 ◽  
Author(s):  
A. Traoré ◽  
I. Baudrimont ◽  
S. Ambaliou ◽  
S.D. Dano ◽  
E.E. Creppy
Keyword(s):  
Cell Cycle ◽  
Epithelial Cell ◽  
Cell Line ◽  
Okadaic Acid ◽  
Epithelial Cell Line ◽  
Colonic Epithelial Cell ◽  
Dna Breaks ◽  
Cycle Arrest ◽  
Colonic Epithelial Cell Line ◽  
Human Colonic Epithelial Cell

scholarly journals A redox-based mechanism for induction of interleukin-1 production by nitric oxide in a human colonic epithelial cell line (HT29-Cl.16E)

1996 ◽  
Vol 313 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Geneviève VALLETTE ◽  
Anne JARRY ◽  
Jean-Eric BRANKA ◽  
Christian L. LABOISSE
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cell ◽  
Cell Line ◽  
Interleukin 1 ◽  
Epithelial Cell Line ◽  
Dependent Manner ◽  
Colonic Epithelial Cell ◽  
Colonic Epithelial Cell Line ◽  
Dose Dependent ◽  
Human Colonic Epithelial Cell

We evaluated the effects of two NO donors, sodium nitroprusside (SNP) and 3-morpholino-sydnonimine (SIN-1), characterized by alternative redox states, i.e. nitrosonium ion (NO+) and nitric oxide (NO•) respectively, on intracellular interleukin-1 (IL-1) production, by a human colonic epithelial cell line (HT29-Cl.16E). SNP was able to induce intracellular IL-1α production up to 10 h incubation, in a dose-dependent manner. Several experiments provide evidence that the NO+ redox form, and not the free radical NO•, is implicated in the IL-1α production: (i) SIN-1, devoid of any NO+ character, led to a very weak IL-1 production as compared with SNP; (ii) the reductive action of a thiol such as cysteine on NO+ led to a dose-dependent increase in NO• concentration, measured as NO2-/NO3- accumulation, and to a large decrease in IL-1 production. Dibutyryl cGMP had no effect on IL-1 production, this finding supporting the concept that a cGMP-independent pathway is involved in the intracellular signalling of NO+. Together these results point out that NO, depending on its redox form, is able to modulate IL-1 production in cultured colonic epithelial cells.

Regulation of chemo/cytokines by somatostatin in a human colonic epithelial cell line

2000 ◽  
Vol 118 (4) ◽  
pp. A93
Author(s):  
Taral Patel ◽  
Ping Jiang ◽  
Sheharyar Ali ◽  
Mark W. Babyatsky
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Colonic Epithelial Cell ◽  
Colonic Epithelial Cell Line ◽  
Human Colonic Epithelial Cell
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