Synthesis of polyacrylamide gel beads with electrostatic functional groups for the molecular imprinting of bovine serum albumin

2005 ◽  
Vol 384 (1) ◽  
pp. 225-230 ◽  
Author(s):  
Xingshou Pang ◽  
Guoxiang Cheng ◽  
Shulai Lu ◽  
Erjun Tang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Functional Groups ◽  
Molecular Imprinting ◽  
Bovine Serum ◽  
Polyacrylamide Gel ◽  
Gel Beads

Soft-wet polyacrylamide gel beads with the imprinting of bovine serum albumin

2006 ◽  
Vol 66 (10) ◽  
pp. 1182-1188 ◽  
Author(s):  
Xingshou Pang ◽  
Guoxiang Cheng ◽  
Yihua Zhang ◽  
Shulai Lu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polyacrylamide Gel ◽  
Gel Beads

Bovine serum albumin-imprinted polyacrylamide gel beads prepared via inverse-phase seed suspension polymerization

2005 ◽  
Vol 550 (1-2) ◽  
pp. 13-17 ◽  
Author(s):  
Xingshou Pang ◽  
Guoxiang Cheng ◽  
Rensheng Li ◽  
Shulai Lu ◽  
Yihua Zhang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Suspension Polymerization ◽  
Polyacrylamide Gel ◽  
Gel Beads

Some aspects of preparative electrophoresis on polyacrylamide gel: Application to bovine serum albumin

Life Sciences ◽  
1964 ◽  
Vol 3 (6) ◽  
pp. 611-615 ◽  
Author(s):  
Aaron M. Altschul ◽  
W.J. Evans ◽  
W.B. Carney ◽  
E.J. McCourtney ◽  
Harry D. Brown
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Polyacrylamide Gel ◽  
Preparative Electrophoresis

RADIOIMMUNOASSAY OF STEROIDS

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S320-S331 ◽  
Author(s):  
A. R. Midgley ◽  
G. D. Niswender
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Steroid Hormones ◽  
Functional Groups ◽  
Inhibitory Activity ◽  
Bovine Serum ◽  
Major Portion ◽  
Steroid Molecule ◽  
Assay Tube

ABSTRACT A number of radioimmunoassay systems have been developed for quantitation of a variety of steroid hormones. Suitable antisera were obtained by immunizing rabbits with steroid derivatives conjugated at various positions to bovine serum albumin. The specificity of the antisera varied directly with the position of conjugation. Changes in the latter resulted in greater than 2000-fold shifts in relative inhibitory activity for a given steroid. The data indicate that conjugation should be done through a region (such as position 6) that is remote from distinguishing functional groups. The specificity data and nature of the inhibition curves suggest that the major portion of the steroid molecule is being recognized as a single immunoreactive unit. The advantages and problems associated with use of radioiodinated conjugates for labeled steroids are discussed. With conjugates, it has been possible to quantitate reliably as little as one pg of some steroids in a single assay tube.

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