Development and optimization of a novel sample preparation method cored on functionalized nanofibers mat-solid-phase extraction for the simultaneous efficient extraction of illegal anionic and cationic dyes in foods

Abstract Background: Because of trace amounts of atrazine in water samples and the complexity of the matrix, direct trace monitoring of atrazine is not feasible by the abovementioned techniques. Hence, an efficient sample pretreatment procedure is necessary for cleanup and preconcentration of atrazine from sample matrices. Objective: In the current paper, a new and efficient sample preparation method named solvent-assisted dispersive solid-phase extraction (SA-DSPE), followed by HPLC–UV, was introduced for the monitoring of atrazine at trace levels in environmental water samples. Methods: In the present method, benzophenone was used as a sorbent for extraction of target molecules. For dispersing solid sorbents in sample solution, very low milligram amounts of benzophenone and dispersive solvent were mixed and fast-injected into the extraction media. A cloudy solution formed, and after interaction of atrazine and the dispersed solid sorbent, the cloudy solution was centrifuged. The extracted atrazine in the solid phase was dissolved in ethanol and analyzed by HPLC–UV. Results: The introduced method showed a low method detection limit (0.1 μg/L), good precision (relative SD: 3.9–6.9%), and appropriate relative recoveries (95–105%). Conclusions: Within this study, a sensitive and reliable method for the quantification of atrazine in wastewater samples was successfully developed. Highlights: The obtained figures of merit for the presented sample preparation method were appropriate. The applicability of the method for analysis of atrazine in real matrices was excellent.

Download Full-text

Analysis of N-methylpyrrolidine in cefepime hydrochloride by ion chromatography using suppressed conductivity detection with solid-phase extraction pre-treatment

Analytical Methods ◽

10.1039/c3ay42041h ◽

2014 ◽

Vol 6 (4) ◽

pp. 1248-1253 ◽

Cited By ~ 3

Author(s):

Nathanael Page ◽

Richard Stevenson ◽

Mark Powell

Keyword(s):

Sample Preparation ◽

Preparation Method ◽

Solid Phase ◽

Hydrolysis Product ◽

Phase Extraction ◽

Sample Preparation Method ◽

Lactam Antibiotic ◽

Suppressed Conductivity Detection ◽

Pre Treatment ◽

The Stability

N-Methylpyrrolidine (NMP) is the principal hydrolysis product of cefepime hydrochloride, a β-lactam antibiotic. Here we develop a sample preparation method for NMP in cefepime hydrochloride which significantly improves the stability of sample solutions.

Download Full-text

Recent Trends in the Development of Green Microextraction Techniques for the Determination of Hazardous Organic Compounds in Wine

Current Analytical Chemistry ◽

10.2174/1573411015666190328185337 ◽

2019 ◽

Vol 15 (7) ◽

pp. 788-800 ◽

Cited By ~ 4

Author(s):

Natasa P. Kalogiouri ◽

Victoria F. Samanidou

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Organic Compounds ◽

Method Development ◽

Solid Phase ◽

Stir Bar Sorptive Extraction ◽

Phase Extraction ◽

Microextraction Techniques ◽

Hazardous Organic Compounds

Background:The sample preparation is the most crucial step in the analytical method development. Taking this into account, it is easily understood why the domain of sample preparation prior to detection is rapidly developing. Following the modern trends towards the automation, miniaturization, simplification and minimization of organic solvents and sample volumes, green microextraction techniques witness rapid growth in the field of food quality and safety. In a globalized market, it is essential to face the consumers need and develop analytical methods that guarantee the quality of food products and beverages. The strive for the accurate determination of organic hazards in a famous and appreciated alcoholic beverage like wine has necessitated the development of microextraction techniques.Objective:The objective of this review is to summarize all the recent microextraction methodologies, including solid phase extraction (SPE), solid phase microextraction (SPME), liquid-phase microextraction (LPME), dispersive liquid-liquid microextraction (DLLME), stir bar sorptive extraction (SBSE), matrix solid-phase dispersion (MSPD), single-drop microextraction (SDME) and dispersive solid phase extraction (DSPE) that were developed for the determination of hazardous organic compounds (pesticides, mycotoxins, colorants, biogenic amines, off-flavors) in wine. The analytical performance of the techniques is evaluated and their advantages and limitations are discussed.Conclusion:An extensive investigation of these techniques remains vital through the development of novel strategies and the implication of new materials that could upgrade the selectivity for the extraction of target analytes.

Download Full-text

Solid-phase extraction of prostanoids using an automatic sample preparation system

Journal of Chromatography A ◽

10.1016/0021-9673(92)87081-i ◽

1992 ◽

Vol 607 (2) ◽

pp. 239-243 ◽

Cited By ~ 4

Author(s):

Georgina Hotter ◽

Gloria Gómez ◽

Isabel Ramis ◽

Gloria Bioque ◽

Joan Roselló-Catafau ◽

...

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Solid Phase ◽

Phase Extraction

Download Full-text

Micro Salting-Out Assisted Matrix Solid-Phase Dispersion: A Simple and Fast Sample Preparation Method for the Analysis of Bisphenol Contaminants in Bee Pollen

Molecules ◽

10.3390/molecules26082350 ◽

2021 ◽

Vol 26 (8) ◽

pp. 2350

Author(s):

Jianing Zhang ◽

Fengjie Yu ◽

Yunmin Tao ◽

Chunping Du ◽

Wenchao Yang ◽

...

Keyword(s):

Sample Preparation ◽

Preparation Method ◽

Solid Phase ◽

Sample Preparation Method ◽

Salting Out ◽

Bee Pollen ◽

Matrix Solid Phase Dispersion ◽

Phase Dispersion ◽

New Strategy ◽

Semi Solid

In the present work, a novel sample preparation method, micro salting-out assisted matrix solid-phase dispersion (μ-SOA-MSPD), was developed for the determination of bisphenol A (BPA) and bisphenol B (BPB) contaminants in bee pollen. The proposed method was designed to combine two classical sample preparation methodologies, matrix solid-phase dispersion (MSPD) and homogenous liquid-liquid extraction (HLLE), to simplify and speed-up the preparation process. Parameters of μ-SOA-MSPD were systematically investigated, and results indicated the significant effect of salt and ACN-H2O extractant on the signal response of analytes. In addition, excellent clean-up ability in removing matrix components was observed when primary secondary amine (PSA) sorbent was introduced into the blending operation. The developed method was fully validated, and the limits of detection for BPA and BPB were 20 μg/kg and 30 μg/kg, respectively. Average recoveries and precisions were ranged from 83.03% to 94.64% and 1.76% to 5.45%, respectively. This is the first report on the analysis of bisphenol contaminants in bee pollen sample, and also on the combination of MSPD and HLLE. The present method might provide a new strategy for simple and fast sample preparation of solid and semi-solid samples.

Download Full-text

Sample Preparation and Extraction in Small Sample Volumes Suitable for Pediatric Clinical Studies: Challenges, Advances, and Experiences of a Bioanalytical HPLC-MS/MS Method Validation Using Enalapril and Enalaprilat

International Journal of Analytical Chemistry ◽

10.1155/2015/796249 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Bjoern B. Burckhardt ◽

Stephanie Laeer

Keyword(s):

Sample Preparation ◽

Solid Phase Extraction ◽

Method Development ◽

Matrix Effects ◽

Solid Phase ◽

Scale Up ◽

Small Sample ◽

Positive Pressure ◽

Phase Extraction ◽

Bioanalytical Method

In USA and Europe, medicines agencies force the development of child-appropriate medications and intend to increase the availability of information on the pediatric use. This asks for bioanalytical methods which are able to deal with small sample volumes as the trial-related blood lost is very restricted in children. Broadly used HPLC-MS/MS, being able to cope with small volumes, is susceptible to matrix effects. The latter restrains the precise drug quantification through, for example, causing signal suppression. Sophisticated sample preparation and purification utilizing solid-phase extraction was applied to reduce and control matrix effects. A scale-up from vacuum manifold to positive pressure manifold was conducted to meet the demands of high-throughput within a clinical setting. Faced challenges, advances, and experiences in solid-phase extraction are exemplarily presented on the basis of the bioanalytical method development and validation of low-volume samples (50 μL serum). Enalapril, enalaprilat, and benazepril served as sample drugs. The applied sample preparation and extraction successfully reduced the absolute and relative matrix effect to comply with international guidelines. Recoveries ranged from 77 to 104% for enalapril and from 93 to 118% for enalaprilat. The bioanalytical method comprising sample extraction by solid-phase extraction was fully validated according to FDA and EMA bioanalytical guidelines and was used in a Phase I study in 24 volunteers.

Download Full-text