scholarly journals Molecular Evolution of the Puroindoline-a, Puroindoline-b, and Grain Softness Protein-1 Genes in the Tribe Triticeae

2006 ◽  
Vol 63 (5) ◽  
pp. 718-718 ◽  
Author(s):  
Alicia N. Massa ◽  
Craig F. Morris
Keyword(s):  
Molecular Evolution ◽  
Grain Softness ◽  
Puroindoline A ◽  
Tribe Triticeae ◽  
Grain Softness Protein

Puroindoline B Limits Binding of Puroindoline A to Starch and Grain Softness

Crop Science ◽  
2006 ◽  
Vol 46 (4) ◽  
pp. 1656-1665 ◽  
Author(s):  
C. G. Swan ◽  
F. D. Meyer ◽  
A. C. Hogg ◽  
J. M. Martin ◽  
M. J. Giroux
Keyword(s):  
Grain Softness ◽  
Puroindoline A

The Nor-D3 locus of Triticum tauschii: natural variation and genetic linkage to markers in chromosome 5

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 387-395 ◽  
Author(s):  
E. S. Lagudah ◽  
R. Appels ◽  
D. McNeil
Keyword(s):  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
D Genome ◽  
Triticum Tauschii ◽  
Intergenic Spacer Region ◽  
Grain Softness ◽  
High Level ◽  
Rna Genes ◽  
Rdna Polymorphism ◽  
Grain Softness Protein

Variation in the intergenic spacer region of the ribosomal RNA genes (located at the Nor locus) was assayed in a collection of 411 accessions of Triticum tauschii from Turkey, USSR, Iran, Afghanisan, Pakistan, and China. Twenty rDNA genotypes were identified and it was demonstrated that T. tauschii accessions from the USSR and Iran have the highest diversity at the Nor locus. At least four of the rDNA genotypes were demonstrated to be alleles of a single major locus, in segregating F2 progeny analyses. The TaqI restriction fragment associated with rDNA genotype 7 was shown to be the same as the Nor-D3a allele present in all bread wheats (based on chromosome location and length of the intergenic spacer region). This genotype was significantly associated with T. t. ssp. strangulata, previously argued to be the donor of the D genome to hexaploid wheat. The Nor locus showed a high level of recombination with the 5SDna-2 locus, which was also located on chromosome 5D. The Nor locus is placed distal to the 5SDna-2 locus but proximal to the grain softness protein gene (XGsp) on the short arm of chromosome 5D.Key words: D genome, Nor-D3, rDNA polymorphism, chromosomal location.

A review of the occurrence of Grain softness protein-1 genes in wheat (Triticum aestivum L.)

2013 ◽  
Vol 83 (6) ◽  
pp. 507-521 ◽  
Author(s):  
Craig F. Morris ◽  
Hongwei Geng ◽  
Brian S. Beecher ◽  
Dongyun Ma
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
Grain Softness ◽  
Grain Softness Protein

scholarly journals Cloning of a wheat 15-kDa grain softness protein (GSP). GSP is a mixture of puroindoline-like polypeptides

1994 ◽  
Vol 223 (3) ◽  
pp. 917-925 ◽  
Author(s):  
Sadequr RAHMAN ◽  
Christopher J. JOLLY ◽  
John H. SKERRITT ◽  
Andrea WALLOSHECK
Keyword(s):  
Grain Softness ◽  
Grain Softness Protein

The Ha locus of wheat: Identification of a polymorphic region for tracing grain hardness in crosses

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1242-1250 ◽  
Author(s):  
M Turner ◽  
Y Mukai ◽  
P Leroy ◽  
B Charef ◽  
R Appels ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Hexaploid Wheat ◽  
Aegilops Tauschii ◽  
Grain Hardness ◽  
High Sequence Identity ◽  
D Genome ◽  
Sequence Identity ◽  
Group 5 ◽  
Grain Softness ◽  
Puroindoline A

The grain softness proteins or friabilins are known to be composed of three main components: puroindoline a, puroindoline b, and GSP-1. cDNAs for GSP-1 have previously been mapped to group-5 chromosomes and their location on chromosome 5D is closely linked to the grain hardness (Ha) locus of hexaploid wheat. A genomic DNA clone containing the GSP-1 gene (wGSP1-A1) from hexaploid wheat has been identified by fluorescent in situ hybridization as having originated from the distal end of the short arm of chromosome 5A. A genomic clone containing the gene (wGSP1-D1) was also isolated from Aegilops tauschii, the donor of the D genome to bread wheat. There are no introns in the GSP-1 genes, and there is high sequence identity between wGSP1-A1 and wGSP1-D1 up to 1 kb 5' and 300 bp 3' to wGSP1-D1. However, regions further upstream and downstream of wGSP1-D1 share no significant sequence identity to corresponding sequences in wGSP1-A1. These regions therefore identified potentially valuable sequences for tracing the Ha locus through assaying polymorphic DNA sequences. The sequence from 300 to 500 bp 3' to wGSP1-D1 (wGSP1-D13) was mapped to the Ha locus in a mapping population. wGSP1-D13 was also tightly linked to genes for puroindoline a and puroindoline b which have been previously mapped to be at the Ha locus. In addition wGSP1-D13 was used to detect RFLPs between near isogenic soft and hard Falcon lines and in a random selection of soft and hard wheats.Key words: wheat, grain hardness, chromosome 5, puroindoline, GSP-1.

Variation in puroindoline polypeptides in Australian wheat cultivars in relation to grain hardness

2000 ◽  
Vol 27 (2) ◽  
pp. 153 ◽  
Author(s):  
Kym-Marie Turnbull ◽  
Therese Gaborit ◽  
Didier Marion ◽  
Sadequr Rahman
Keyword(s):  
Protein Sequence ◽  
Sequence Variation ◽  
Wheat Cultivars ◽  
Grain Hardness ◽  
Australian Wheat ◽  
Grain Softness ◽  
Puroindoline A ◽  
Selection Of ◽  
Do So

The sequence of the puroindoline-b gene from 15 Australian wheat cultivars was determined. Sequence variation was observed in the WPTKWWKGGCE motif of the deduced puroindoline-b protein sequence. Previously, it has been suggested that this sequence is crucial in determining grain hardness. In this study, no correlation was found between the variation in this sequence and the hardness or softness of the cultivar. The amounts of puroindo-line- a and puroindoline-b protein in a selection of hard and soft Australian wheat cultivars were also determined using ELISA techniques. Both soft and hard cultivars had variable amounts of puroindoline-a and puroindoline-b. In particular, it is notable that the hard cultivars Cook and Diaz contained high amounts of puroindoline-a and puroin-doline- b and also contained the puroindoline-b sequence previously reported to be associated with grain softness. These results suggest that if the puroindoline proteins are involved in determining grain softness or hardness they do so as part of a multi-component mechanism.

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