Variation in puroindoline polypeptides in Australian wheat cultivars in relation to grain hardness

2000 ◽  
Vol 27 (2) ◽  
pp. 153 ◽  
Author(s):  
Kym-Marie Turnbull ◽  
Therese Gaborit ◽  
Didier Marion ◽  
Sadequr Rahman
Keyword(s):  
Protein Sequence ◽  
Sequence Variation ◽  
Wheat Cultivars ◽  
Grain Hardness ◽  
Australian Wheat ◽  
Grain Softness ◽  
Puroindoline A ◽  
Selection Of ◽  
Do So

The sequence of the puroindoline-b gene from 15 Australian wheat cultivars was determined. Sequence variation was observed in the WPTKWWKGGCE motif of the deduced puroindoline-b protein sequence. Previously, it has been suggested that this sequence is crucial in determining grain hardness. In this study, no correlation was found between the variation in this sequence and the hardness or softness of the cultivar. The amounts of puroindo-line- a and puroindoline-b protein in a selection of hard and soft Australian wheat cultivars were also determined using ELISA techniques. Both soft and hard cultivars had variable amounts of puroindoline-a and puroindoline-b. In particular, it is notable that the hard cultivars Cook and Diaz contained high amounts of puroindoline-a and puroin-doline- b and also contained the puroindoline-b sequence previously reported to be associated with grain softness. These results suggest that if the puroindoline proteins are involved in determining grain softness or hardness they do so as part of a multi-component mechanism.

Identification of Australian wheat cultivars by laboratory procedures: examination of pure samples of grain

1974 ◽  
Vol 14 (71) ◽  
pp. 796 ◽  
Author(s):  
CW Wrigley ◽  
KW Shepherd
Keyword(s):  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Wheat Cultivars ◽  
Grain Hardness ◽  
Additional Information ◽  
Size Index ◽  
Starch Gel ◽  
Australian Wheat ◽  
Laboratory Procedures ◽  
Single Grain

Three laboratory procedures have been examined for the identification of about fifty wheat cultivars currently grown in Australia. The most discriminating of these methods is starch gel electrophoresis of gliadin proteins extracted from a single grain or from meal. This procedure is capable of identifying many of the cultivars directly. However, in some cases identification is complicated by the observation of more than one biotype for a cultivar on the basis of this test. By comparison, a larger number of grains can be examined by the qualitative phenol test but it is less discriminating. Additional information is provided by applying the test to glumes. Thirdly, quantitative assessment of grain hardness, measuring either particle size index or pearling resistance, gives a division of cultivars into about five groups. Specific results are listed for all methods so that the most suitable procedure can be chosen for distinguishing a particular group of cultivars.

The Ha locus of wheat: Identification of a polymorphic region for tracing grain hardness in crosses

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1242-1250 ◽  
Author(s):  
M Turner ◽  
Y Mukai ◽  
P Leroy ◽  
B Charef ◽  
R Appels ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Hexaploid Wheat ◽  
Aegilops Tauschii ◽  
Grain Hardness ◽  
High Sequence Identity ◽  
D Genome ◽  
Sequence Identity ◽  
Group 5 ◽  
Grain Softness ◽  
Puroindoline A

The grain softness proteins or friabilins are known to be composed of three main components: puroindoline a, puroindoline b, and GSP-1. cDNAs for GSP-1 have previously been mapped to group-5 chromosomes and their location on chromosome 5D is closely linked to the grain hardness (Ha) locus of hexaploid wheat. A genomic DNA clone containing the GSP-1 gene (wGSP1-A1) from hexaploid wheat has been identified by fluorescent in situ hybridization as having originated from the distal end of the short arm of chromosome 5A. A genomic clone containing the gene (wGSP1-D1) was also isolated from Aegilops tauschii, the donor of the D genome to bread wheat. There are no introns in the GSP-1 genes, and there is high sequence identity between wGSP1-A1 and wGSP1-D1 up to 1 kb 5' and 300 bp 3' to wGSP1-D1. However, regions further upstream and downstream of wGSP1-D1 share no significant sequence identity to corresponding sequences in wGSP1-A1. These regions therefore identified potentially valuable sequences for tracing the Ha locus through assaying polymorphic DNA sequences. The sequence from 300 to 500 bp 3' to wGSP1-D1 (wGSP1-D13) was mapped to the Ha locus in a mapping population. wGSP1-D13 was also tightly linked to genes for puroindoline a and puroindoline b which have been previously mapped to be at the Ha locus. In addition wGSP1-D13 was used to detect RFLPs between near isogenic soft and hard Falcon lines and in a random selection of soft and hard wheats.Key words: wheat, grain hardness, chromosome 5, puroindoline, GSP-1.

scholarly journals Selection of Tree Nut Allergen Peptide Markers: A Need for Improved Protein Sequence Databases

2019 ◽  
Vol 102 (5) ◽  
pp. 1263-1270 ◽  
Author(s):  
Weili Xiong ◽  
Melinda A McFarland ◽  
Cary Pirone ◽  
Christine H Parker
Keyword(s):  
Food Allergen ◽  
Protein Sequence ◽  
Sequence Information ◽  
Sequencing Data ◽  
Reference Tree ◽  
Candidate Peptide ◽  
Tree Nut ◽  
Allergen Detection ◽  
Sequence Databases ◽  
Selection Of

Abstract Background: To effectively safeguard the food-allergic population and support compliance with food-labeling regulations, the food industry and regulatory agencies require reliable methods for food allergen detection and quantification. MS-based detection of food allergens relies on the systematic identification of robust and selective target peptide markers. The selection of proteotypic peptide markers, however, relies on the availability of high-quality protein sequence information, a bottleneck for the analysis of many plant-based proteomes. Method: In this work, data were compiled for reference tree nut ingredients and evaluated using a parsimony-driven global proteomics workflow. Results: The utility of supplementing existing incomplete protein sequence databases with translated genomic sequencing data was evaluated for English walnut and provided enhanced selection of candidate peptide markers and differentiation between closely related species. Highlights: Future improvements of protein databases and release of genomics-derived sequences are expected to facilitate the development of robust and harmonized LC–tandem MS-based methods for food allergen detection.

Catalog Design: Design Using Available Assets

1992 ◽  
Author(s):  
S. Vadde ◽  
J. K. Allen ◽  
F. Mistree
Keyword(s):  
Deterministic Model ◽  
Limited Resources ◽  
Complex Task ◽  
Cooling Fluid ◽  
Selection Problems ◽  
Catalog Design ◽  
Standard Framework ◽  
Selection Of ◽  
Do So

Abstract Catalog design is a procedure in which a system is assembled by selecting standard components from catalogs of available components. Selection in design involves making a choice among a number of alternatives taking into account several attributes. The information available to a designer to do so during the early stages of project initiation may be uncertain. The uncertainty in information may be imprecise or stochastic. Under these circumstances, a designer has to balance limited resources against the quality of solution obtained or decisions made by accounting for uncertainty in information available. This complex task becomes formidable when dealing with coupled selection problems, that is problems that should be solved simultaneously. Coupled selection problems share a number of coupling attributes among them. In an earlier paper we have shown how selection problems, both coupled and uncoupled can be reformulated as a single compromise Decision Support Problem (DSP) using a deterministic model. In this paper, we show how the traditional compromise DSP can be extended to represent a nondeterministic case. We use fuzzy set theory to model imprecision and Bayesian statistics to model stochastic information. Formulations that can be solved with the same solution scheme are presented to handle both fuzzy and stochastic information in the standard framework of a compromise DSP. The approaches are illustrated by an example involving the coupled selection of a heat exchanger concept and a cooling fluid for a specific application. The emphasis in this paper is placed on explaining the methods.

scholarly journals TALKING ABOUT RAPE – AND WHY IT MATTERS: Adjudicating rape in the Western Cape High Court

2016 ◽  
Author(s):  
Stacy Moreland
Keyword(s):  
Criminal Law ◽  
Institutional Factors ◽  
Western Cape ◽  
Sexual Offences ◽  
High Court ◽  
The Law ◽  
The Relationship ◽  
Selection Of ◽  
Do So

This article asks the question: how do judges know what rape is and what it is not? The statutory definition contained in the Criminal Law (Sexual Offences and Related Matters) Amendment Act1 (SORMA) guides courts in adjudicating rape cases, and as such the definition is theirs to interpret and implement. This article analyses a small selection of recent judgements of the Western Cape High Court2 (WCHC) for answers. The article begins by establishing why judgements are an important source for understanding what rape means in society at large; it then discusses the relationship between power, language, and the law. This is followed by specific analyses of cases that show how patriarchy still defines how judges express themselves about rape. It concludes by looking at the institutional factors that discourage judges from adopting new ways of talking about rape, and their constitutional mandate to do so.

scholarly journals Diurnal Variations in Physiological Characteristics, Photoassimilates, and Total Ascorbate in Early and Late Sown Indian Wheat Cultivars under Exposure to Elevated Ozone

Atmosphere ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1568
Author(s):  
Durgesh Singh Yadav ◽  
Bhavna Jaiswal ◽  
Shashi Bhushan Agrawal ◽  
Madhoolika Agrawal
Keyword(s):  
Ascorbic Acid ◽  
Ascorbic Acid Content ◽  
Triticum Aestivum L ◽  
Diurnal Changes ◽  
Wheat Cultivars ◽  
Negative Effects ◽  
Breeding Programs ◽  
Elevated O3 ◽  
Elevated Ozone ◽  
Selection Of

Rising tropospheric ozone (O3) in the atmosphere is detrimental to crop’s productivity and is one of the reasons for a warmer climate. The present study describes diurnal changes in gaseous exchange, chlorophyll fluorescence, ascorbic acid, and photoassimilate parameters in flag leaves of four Indian wheat (Triticum aestivum L.) cultivars (two early sown and two late sown cultivars) under ambient and elevated O3 treatments, using the open-top chambers (OTCs). Results showed that the diurnal pattern of photosynthetic rate (Ps), sucrose, and ascorbic acid content varied according to changes in photosynthetically active radiation (PAR) and O3 concentrations during the daytime and were maximum between 10:00 to 12:00. The present study suggested that elevated O3 caused more negative effects on photosystem II in early sown compared to late sown cultivars. The greater loss of photosynthesis led to lower production of photoassimilates in early sown cultivars, which utilized more assimilates in ascorbic acid formation for detoxification of ROS formed due to elevated O3. This work will also help to identify the robustness of physiological machinery in different wheat cultivars under elevated levels of O3, and may be used for selection of suitable cultivars during future breeding programs.

The hardness locus in Australian wheat lines

2001 ◽  
Vol 52 (12) ◽  
pp. 1275 ◽  
Author(s):  
B. G. Osborne ◽  
K. M. Turnbull ◽  
R. S. Anderssen ◽  
S. Rahman ◽  
P. J. Sharp ◽  
...  
Keyword(s):  
Chromosome Region ◽  
Genetic Locus ◽  
Quantitative Information ◽  
Grain Weight ◽  
Limited Information ◽  
Grain Hardness ◽  
Hardness Locus ◽  
Australian Wheat ◽  
Wheat Lines ◽  
Cut Surface

The genetic factors that determine grain hardness in Australian wheat (Triticum aestivum L.) germplasm were investigated by studying the grain from 4 crosses (160–180 lines per cross). Although not all the crosses were between hard and soft wheats, the doubled haploid lines derived from the crosses showed significant variation in hardness as assessed either by the Single-Kernel Characterisation System (SKCS 4100) or the scanning electron microscopy appearance of cut surfaces. The wheat cultivars used in the study were Cranbrook, Halberd, CD87, Katepwa, Sunco, Tasman, Egret, and Sunstar and of these only Egret is normally regarded as soft. The quantitative information from the SKCS 4100 was integrated into the genetic map information established for the Cranbrook Halberd, CD87 Katepwa, and Sunco Tasman crosses. For the Egret Sunstar cross, the limited information available from the positioning of genetic markers was used to specifically examine the linkage between the hardness trait and the Pina-D1 (puroindoline) genetic locus on 5DS, and very close linkage was established. The Egret Sunstar cross was also used to develop a more rigorous rheologically based analysis of the raw SKCS crush response data. In addition, the cut surface of the lines was analysed and most (98%) of the samples showed a genetic linkage between the appearance of the cut surface (related to vitreousness) and the SKCS hardness index. Among the other crosses only Cranbrook Halberd showed linkage of the hardness trait to the previously identified hardness locus (ha) located on 5DS as defined by DNA markers for the Pina-D1 locus, and the microsatellite wmc233. The statistical association was shown to be highly significant, with approximately 30% of the variation accounted for by the 5DS region. Another region on chromosome 4D showed a significant association in the Cranbrook Halberd cross. The CD87 Katepwa cross did not show any consistent associations between the SKCS measures and chromosome region, whereas in the Sunco Tasman cross a highly significant association only on chromosome 4B (accounting for 20% of the variation) was suggested. The Sunco Tasman cross showed an overlap of the chromosome region that accounted for variation in both grain weight and hardness and this influence of grain weight on hardness was independently confirmed by a detailed qualitative rheological analysis of the crush response profiles for the Egret Sunstar lines. It is evident from the study that, in Australian wheat lines, there are some major effects on grain hardness that are not associated with the classical ha locus located on 5DS.

scholarly journals Goal-directed and stimulus-driven selection of internal representations

2020 ◽  
Vol 117 (39) ◽  
pp. 24590-24598
Author(s):  
Freek van Ede ◽  
Alexander G. Board ◽  
Anna C. Nobre
Keyword(s):  
Working Memory ◽  
Feature Matching ◽  
Memory Performance ◽  
Sensory Information ◽  
Internal Representations ◽  
Memory Representations ◽  
External Stimuli ◽  
Selection Of ◽  
Do So

Adaptive behavior relies on the selection of relevant sensory information from both the external environment and internal memory representations. In understanding external selection, a classic distinction is made between voluntary (goal-directed) and involuntary (stimulus-driven) guidance of attention. We have developed a task—the anti-retrocue task—to separate and examine voluntary and involuntary guidance of attention to internal representations in visual working memory. We show that both voluntary and involuntary factors influence memory performance but do so in distinct ways. Moreover, by tracking gaze biases linked to attentional focusing in memory, we provide direct evidence for an involuntary “retro-capture” effect whereby external stimuli involuntarily trigger the selection of feature-matching internal representations. We show that stimulus-driven and goal-directed influences compete for selection in memory, and that the balance of this competition—as reflected in oculomotor signatures of internal attention—predicts the quality of ensuing memory-guided behavior. Thus, goal-directed and stimulus-driven factors together determine the fate not only of perception, but also of internal representations in working memory.

Puroindoline B Limits Binding of Puroindoline A to Starch and Grain Softness

Crop Science ◽  
2006 ◽  
Vol 46 (4) ◽  
pp. 1656-1665 ◽  
Author(s):  
C. G. Swan ◽  
F. D. Meyer ◽  
A. C. Hogg ◽  
J. M. Martin ◽  
M. J. Giroux
Keyword(s):  
Grain Softness ◽  
Puroindoline A
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