The role of intracellular and extracellular calcium in mechanical and intracellular electrical activity of human urinary bladder smooth muscle

2000 ◽  
Vol 28 (4) ◽  
pp. 260-268 ◽  
Author(s):  
A. J. Visser ◽  
R. van Mastrigt
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Electrical Activity ◽  
Extracellular Calcium ◽  
Bladder Smooth Muscle ◽  
Human Urinary Bladder ◽  
Urinary Bladder Smooth Muscle

scholarly journals Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle

2003 ◽  
Vol 138 (5) ◽  
pp. 757-766 ◽  
Author(s):  
Alexandra Wibberley ◽  
Zunxuan Chen ◽  
Erding Hu ◽  
J Paul Hieble ◽  
Timothy D Westfall
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Functional Role ◽  
Rho Kinase ◽  
Bladder Smooth Muscle ◽  
Rat Urinary Bladder ◽  
Urinary Bladder Smooth Muscle

Generation of a human urinary bladder smooth muscle cell line

2012 ◽  
Vol 48 (2) ◽  
pp. 84-96 ◽  
Author(s):  
Yongmu Zheng ◽  
Shaohua Chang ◽  
Ettickan Boopathi ◽  
Sandra Burkett ◽  
Mary John ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Smooth Muscle Cell ◽  
Cell Line ◽  
Muscle Cell ◽  
Bladder Smooth Muscle ◽  
Human Urinary Bladder ◽  
Urinary Bladder Smooth Muscle ◽  
Bladder Smooth Muscle Cell ◽  
Muscle Cell Line

Human urinary bladder smooth muscle is dependent on membrane cholesterol for cholinergic activation

2010 ◽  
Vol 634 (1-3) ◽  
pp. 142-148 ◽  
Author(s):  
Yulia Shakirova ◽  
Michiko Mori ◽  
Mari Ekman ◽  
Jonas Erjefält ◽  
Bengt Uvelius ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Membrane Cholesterol ◽  
Bladder Smooth Muscle ◽  
Human Urinary Bladder ◽  
Urinary Bladder Smooth Muscle ◽  
Cholinergic Activation

scholarly journals Stimulation of β3-adrenoceptors relaxes rat urinary bladder smooth muscle via activation of the large-conductance Ca2+-activated K+ channels

2008 ◽  
Vol 295 (5) ◽  
pp. C1344-C1353 ◽  
Author(s):  
Kiril L. Hristov ◽  
Xiangli Cui ◽  
Sean M. Brown ◽  
Lei Liu ◽  
Whitney F. Kellett ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Bk Channels ◽  
Bk Channel ◽  
Bladder Smooth Muscle ◽  
Rat Urinary Bladder ◽  
Urinary Bladder Smooth Muscle ◽  
Brl 37344 ◽  
Stimulation Of

We investigated the role of large-conductance Ca2+-activated K+ (BK) channels in β3-adrenoceptor (β3-AR)-induced relaxation in rat urinary bladder smooth muscle (UBSM). BRL 37344, a specific β3-AR agonist, inhibits spontaneous contractions of isolated UBSM strips. SR59230A, a specific β3-AR antagonist, and H89, a PKA inhibitor, reduced the inhibitory effect of BRL 37344. Iberiotoxin, a specific BK channel inhibitor, shifts the BRL 37344 concentration response curves for contraction amplitude, net muscle force, and tone to the right. Freshly dispersed UBSM cells and the perforated mode of the patch-clamp technique were used to determine further the role of β3-AR stimulation by BRL 37344 on BK channel activity. BRL 37344 increased spontaneous, transient, outward BK current (STOC) frequency by 46.0 ± 20.1%. In whole cell mode at a holding potential of Vh = 0 mV, the single BK channel amplitude was 5.17 ± 0.28 pA, whereas in the presence of BRL 37344, it was 5.55 ± 0.41 pA. The BK channel open probability was also unchanged. In the presence of ryanodine and nifedipine, the current-voltage relationship in response to depolarization steps in the presence and absence of BRL 37344 was identical. In current-clamp mode, BRL 37344 caused membrane potential hyperpolarization from −26.1 ± 2.1 mV (control) to −29.0 ± 2.2 mV. The BRL 37344-induced hyperpolarization was eliminated by application of iberiotoxin, tetraethylammonium or ryanodine. The data indicate that stimulation of β3-AR relaxes rat UBSM by increasing the BK channel STOC frequency, which causes membrane hyperpolarization and thus relaxation.

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