Whole-Cell MALDI-TOF Mass Spectrometry and Multilocus Sequence Analysis in the Discrimination of Pseudomonas stutzeri Populations: Three Novel Genomovars

2013 ◽  
Vol 66 (3) ◽  
pp. 522-532 ◽  
Author(s):  
Claudia Scotta ◽  
Margarita Gomila ◽  
Magdalena Mulet ◽  
Jorge Lalucat ◽  
Elena García-Valdés
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Pseudomonas Stutzeri ◽  
Multilocus Sequence Analysis ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals The use of ITS DNA sequence analysis and MALDI-TOF mass spectrometry in diagnosing an infection withFusarium proliferatum

2008 ◽  
Vol 17 (11) ◽  
pp. 965-971 ◽  
Author(s):  
Florian Seyfarth ◽  
Mirjana Ziemer ◽  
Herbert G. Sayer ◽  
Anke Burmester ◽  
Marcel Erhard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization

2013 ◽  
pp. 197-209 ◽  
Author(s):  
Richard Ouedraogo ◽  
Julien Textoris ◽  
Aurélie Daumas ◽  
Christian Capo ◽  
Jean-Louis Mege
Keyword(s):  
Mass Spectrometry ◽  
Immune Cell ◽  
Cell Analysis ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Intact-Cell MALDI-ToF Mass Spectrometry for the Authentication of Drug-Adapted Cancer Cell Lines

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1194 ◽  
Author(s):  
Jane F. Povey ◽  
Emily Saintas ◽  
Adewale V. Aderemi ◽  
Florian Rothweiler ◽  
Richard Zehner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Line ◽  
Cell Lines ◽  
Mammalian Cells ◽  
Intact Cell ◽  
Mass Spectrometry Analysis ◽  
Genetic Origin ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

The use of cell lines in research can be affected by cell line misidentification. Short tandem repeat (STR) analysis is an effective method, and the gold standard, for the identification of the genetic origin of a cell line, but methods that allow the discrimination between cell lines of the same genetic origin are lacking. Here, we use intact cell MALDI-ToF mass spectrometry analysis, routinely used for the identification of bacteria in clinical diagnostic procedures, for the authentication of a set of cell lines consisting of three parental neuroblastoma cell lines (IMR-5, IMR-32 and UKF-NB-3) and eleven drug-adapted sublines. Principal component analysis (PCA) of intact-cell MALDI-ToF mass spectrometry data revealed clear differences between most, but not all, of the investigated cell lines. Mass spectrometry whole-cell fingerprints enabled the separation of IMR-32 and its clonal subline IMR-5. Sublines that had been adapted to closely related drugs, for example, the cisplatin- and oxaliplatin-resistant UKF-NB-3 sublines and the vincristine- and vinblastine-adapted IMR-5 sublines, also displayed clearly distinctive patterns. In conclusion, intact whole-cell MALDI-ToF mass spectrometry has the potential to be further developed into an authentication method for mammalian cells of a common genetic origin.

Analysis and Characterization of Immune Cells and Their Activation Status by Whole-Cell MALDI-TOF Mass Spectrometry

2019 ◽  
pp. 339-351 ◽  
Author(s):  
Richard Ouedraogo ◽  
Julien Textoris ◽  
Laurent Gorvel ◽  
Aurélie Daumas ◽  
Christian Capo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Cells ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Activation Status

scholarly journals Utilization of Whole-Cell MALDI-TOF Mass Spectrometry to Differentiate Burkholderia pseudomallei Wild-Type and Constructed Mutants

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0144128 ◽  
Author(s):  
Suthamat Niyompanich ◽  
Kitima Srisanga ◽  
Janthima Jaresitthikunchai ◽  
Sittiruk Roytrakul ◽  
Sumalee Tungpradabkul
Keyword(s):  
Mass Spectrometry ◽  
Burkholderia Pseudomallei ◽  
Wild Type ◽  
Whole Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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